[1]李玉珍,肖怀秋,杨 涛,等.响应面优化低值豆粕液态制备多肽工艺[J].大豆科学,2012,31(04):649-654.[doi:10.3969/j.issn.1000-9841.2012.04.028]
　LI Yu-zhen,XIAO Huai-qiu,YANG Tao,et al.Response Surface Methodology for Optimization of Soybean Peptide Production Technology from Low-valued Soybean Meal[J].Soybean Science,2012,31(04):649-654.[doi:10.3969/j.issn.1000-9841.2012.04.028]
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响应面优化低值豆粕液态制备多肽工艺

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年04期 页码: 649-654 栏目: 出版日期: 2012-08-25
Title:
Response Surface Methodology for Optimization of Soybean Peptide Production Technology from Low-valued Soybean Meal
文章编号:
1000-9841（2012）04-0649-06
作者:
李玉珍1; 肖怀秋1; 杨 涛2; 林亲录2
1.湖南化工职业技术学院 应用化学系，湖南 株洲 412004；
2.中南林业科技大学 食品科学与工程学院，湖南 长沙 410004
Author(s):
LI Yu-zhen1; XIAO Huai-qiu1; YANG Tao2; LIN Qin-lu2
1.Applied Chemistry Department of Hunan Chemical Industry and Vocational Technology College,Zhuzhou 412004,Hunan；
2.Food Science and Engineering College,Central South University of Forestry and Technology，Changsha 410004,Hunan,China
关键词:
豆粕; 大豆多肽; 中心复合设计; 响应面分析; 降维分析
Keywords:
Soybean meal; Soybean peptide; Central composite design; Response surface methodology; Dimension reduction analysis
分类号:
TS201.3
DOI:
10.3969/j.issn.1000-9841.2012.04.028
文献标志码:
A
摘要:
为优化低值豆粕液态发酵生产大豆多肽工艺，应用Minimum Run Equireplicated Res IV析因设计进行了主效因子的筛选，根据主效因子影响及变化方向进行爬陡坡试验，最后，应用二次旋转中心复合响应面设计对液态发酵多肽工艺进行了优化，优化工艺条件为豆粕浓度6.0%、pH 8.0、装瓶量93.0 mL·300 mL-1。最优条件下模型预测多肽含量为707.204 μg·mL-1，验证试验结果为683.023±9.23 μg·mL-1(n=6)。
Abstract:
To investigate the optimal producing conditions of soybean peptide(SBP)from soybean meal(SBM)by liquid state fermentation(LSF),Minimum Run Equireplicated Res IV design was used to screen main factors affecting SBP production,steepest ascent experiment was adopted to approach the optimal region based the results from the factorial design.At last,quadratic rotating central composite design(QRCCD)was applied to optimize the SBP producing parameters.The optimal condition for SBP biosynthesis are 6.0% of SBM,pH 8.0 and media amount is 93.0 mL·300 mL-1.The predicted value and actual value of SBP are 707.204 μg·mL-1 and 683.023±9.23 μg·mL-1(n=6),respectively.

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备注/Memo

基金项目：国家自然科学基金资助项目 (31050012)。

第一作者简介：李玉珍（1981-），女，讲师，硕士，研究方向为蛋白质生物化学。E-mail:yuzhenli@163.com。

更新日期/Last Update: 2014-08-18