[1]刘 杰,周 波,杨春燕,等.抗草甘膦转EPSPS大豆的基因漂移研究[J].大豆科学,2012,31(04):517-521.[doi:10.3969/j.issn.1000-9841.2012.04.002]
　LIU Jie,ZHOU Bo,YANG Chun-yan,et al.Gene Flowing of Genetically Modified Glyphosate-Resistant Soybean with EPSPS [J].Soybean Science,2012,31(04):517-521.[doi:10.3969/j.issn.1000-9841.2012.04.002]
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抗草甘膦转EPSPS大豆的基因漂移研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年04期 页码: 517-521 栏目: 出版日期: 2012-08-25
Title:
Gene Flowing of Genetically Modified Glyphosate-Resistant Soybean with EPSPS
文章编号:
1000-9841（2012）04-0517-05
作者:
刘 杰1; 2; 周 波3; 杨春燕4; 李玉荣4; 蒋凌雪2; 3; 张孟臣4; 陶 波3; 邱丽娟2
1.南昌大学 生命科学与食品工程学院，江西 南昌 330031;
2.中国农业科学院 作物科学研究所/国家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室，北京 100081;
3.东北农业大学 农学院，黑龙江 哈尔滨 150030;
4.河北省农林科学院 粮油作物研究所，河北 石家庄 050051
Author(s):
LIU Jie1; 2; ZHOU Bo3; YANG Chun-yan4; LI Yu-rong4; JIANG Ling-xue2; 3; ZHANG Meng-chen4; TAO Bo3; QIU Li-juan2
1.College of Life Sciences and Food Engineering,Nanchang University,Nanchang 330031,Jiangxi;
2.National Key Facility for Crop Gene Resources and Genetic Impmvement(NFCRI)／Key Laboratory of Germplasm & Biotechnology(MOA)，Institute of Crop Sciences，Chinese Academy of Agricultural Sciences，Beijing 100081;
3.College of agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang;

关键词:
抗草甘膦转基因大豆; 基因漂移; 距离; 方向
Keywords:
Genetically modified glyphosate-resistant soybean; Gene flow; Distance; Direction
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.04.002
文献标志码:
A
摘要:
以抗草甘膦转EPSPS基因大豆和不同生长类型的非转基因大豆为材料，通过表型筛选及PCR分子检测，分析不同方向和距离条件下转基因大豆中外源基因通过花粉向非转基因大豆的漂移率，为制定转基因大豆安全种植隔离措施提供依据。结果表明，不同生长类型大豆的基因漂移率明显不同，以育成品系ZZH015最高，在70 612株中的漂移率为0.45%；半蔓生地方品种DPZ722次之，164 449株中漂移率为0.01%，而195 088株野生大豆YDD080中没有检测到基因漂移。方差分析表明，漂移率在不同距离和方向条件下均有显著差异，随着距离的增加不断递减。在5 m处漂移率为0.03%，而在29 m处降至0.001%。基因漂移在下风口方向发生较多，表明风媒可能是影响基因漂移分布的重要因素。如果以1%基因漂移率为阈值，无论是自然条件下还是随机选取1 000粒检测，漂移率都低于阈值，表明转EPSPS基因大豆的基因漂移可以忽略不计。
Abstract:
The study of gene flow is one of the most important part of assessing the environmental safety of transgenic crops.In order to determine the frequency of gene flow from glyphosate-resistant transgenic soybean to nontransgenic soybeans in field condition and provide evidence for formulating security procedure of transgenic crops planting,we planted released cultivar,semi-rampant landrace and wild soybeans around the pollen donor at six different distances in eight directions.The frequency of gene flow was obviously varied with evolution types of soybean.The average outcrossing rate was 0.01% and 0.45% in 164 449 plants of semi-rampant landrace ‘DPZ722’ and 70 612 plants of selected cultivar ‘ZZH015’，respectively.There was no gene flow in 195 088 plants of wild soybean ‘YDD080’.Analysis of variance showed that the rate of gene flow in different distances and direction were significant.The gene flow frequency declined from 0.03% to 0.001% with the isolated distance increase from 5 m to 29 m.Most of the gene flow occurred under the tuyere.Taking 1% as the threshold,the frequency of gene flow under natural condition or random choose 1 000 plants to test were lower than 1%,which can be ignored.

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备注/Memo

基金项目：转基因玉米小麦大豆环境安全评价技术课题（2011ZX08011-003）。

第一作者简介：刘杰（1986-），女，在读硕士，研究方向为大豆基因资源挖掘。E-mail：liujie7386@126.com。
通讯作者：邱丽娟（1963-），女，研究员，博士生导师，主要从事大豆基因资源挖掘与利用研究。E-mail：qiu_lijuan@263.net。

更新日期/Last Update: 2014-08-18