[1]方 建,楚 楚,程冬萍,等.应用大孔树脂纯化大豆异黄酮的工艺研究[J].大豆科学,2012,31(03):474-477.[doi:10.3969/j.issn.1000-9841.2012.03.029]
　FANG Jian,CHU Chu,CHENG Dong-ping,et al.Purification of Soybean Isoflavones by Macroporous Resin[J].Soybean Science,2012,31(03):474-477.[doi:10.3969/j.issn.1000-9841.2012.03.029]
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应用大孔树脂纯化大豆异黄酮的工艺研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年03期 页码: 474-477 栏目: 出版日期: 2012-03-25
Title:
Purification of Soybean Isoflavones by Macroporous Resin
文章编号:
1000-9841（2012）03-0474-04
作者:
方 建1; 楚 楚1; 程冬萍1; 黄六仔2; 颜继忠1
1.浙江工业大学 药学院，浙江 杭州310014;
2.浙江惠松制药有限公司，浙江 杭州310018
Author(s):
FANG Jian1; CHU Chu1; CHENG Dong-ping1; HUANG Liu-zai2; YAN Ji-zhong1
1.College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,Zhejiang;
2.Zhejiang Huisong Pharmaceutical Co.,Ltd,Hangzhou 310018,Zhejiang,China
关键词:
大豆异黄酮; 超滤; 大孔树脂
Keywords:
Soybean isoflavones; Ultrafiltration; Macroporous resin
分类号:
TQ914.1
DOI:
10.3969/j.issn.1000-9841.2012.03.029
文献标志码:
A
摘要:
先用聚醚砜有机膜对豆粕乙醇提取液进行超滤，然后用D101大孔树脂纯化，研究大豆异黄酮的精制工艺。结果表明：用孔径5 000 D的有机膜超滤处理豆粕乙醇提取液，能够去除其中56.1%的杂质。用D101大孔树脂纯化，采用上样液浓度为0.750 mg·mL-1大豆异黄酮溶液，流速为1.0 BV·h-1，依次用去离子水、1.0 BV 10%乙醇、2.0 BV 80%乙醇淋洗大孔树脂，得到纯度为41.2%的大豆异黄酮产品，大豆异黄酮的总收率为65.7%。
Abstract:
This paper studied the soybean isoflavones refining process by ultrafiltrating soybean meal extract with polyether sulphone organic membrane and then purified with D101 macroporous resin.The detailed soybean isoflavones refining process was studied and optimized conditions were obtained.The results showed that removal rate of the impurities was up to 56.1% by ultrafiltrating soybean ethanol extract with organic membrane of pore diameter 5 000 D.Purified by D101 macroporous resin,with 0.750 mg·mL-1 sample concentration and 1.0 BV·h-1 fluid samples rate,then successively rinsed with 1.0 BV deionized water,1.0 BV 10% ethanol and 2.0 BV 80% ethanol,purify of 41.2% soybean isoflavone products was obtained and the overall yield was 65.7%.

参考文献/References:

[1]Kudou S,Fleury Y,Welti D,et al.Malonyl isoflavone glycosides in soybean seeds[J].Agricultural and Biological Chemistry,1991,55(9):2227-2233.

[2]高荣海,张春红,赵秀红,等.大豆异黄酮研究进展[J].粮食与油脂,2009(5):1-4.(Gao R H,Zhang C H,Zhao X H,et al.Research progress on soybean isoflavone[J].Cereals & Oils,2009(5):1-4.)

[3]陶利,李禾.大豆异黄酮的药理作用[J].解放军药学学报,2011,27(4):360-362.(Tao L,Li H.The pharmacological effects of soybean isoflavones[J].Pharmaceutical Journal of Chinese People’s Liberation Army,2011,27(4):360-362.)

[4]Shaw W,Sayo U,Yuriko K.Isoflavones for prevention of cancer,cardiovascular diseases,gynecological problems and possibleimmune potentiation[J].Biomedicine & Pharmacotherapy,2002,56(6)：302-312.

[5]何伟,李伟.大孔树脂在中药成分分离中的应用[J].南京中医药大学学报,2005,21(2):134-136.(He W,Li W.Application of macroporous resin in separating ingredient from the Chinese native medicine[J].Journal of Nanjing University of Traditional Chinese Medicine,2005,21(2):134-136.)

[6]Ahmad K H,Bob S,John S,et al.Process for producing high purity isoflavones:US,7524526B2[P].2009-04-28.

[7]石彦国,毕小磊,孙冰玉.不同大孔树脂对大豆糖蜜中异黄酮吸附影响研究[J].大豆科技,2010(2):25-28.(Shi Y G,Bi X L,Sun B Y.Effect of different type of macrosporous resins on isoflavones adsorption from soybean molasses[J].Soybean Science & Technology,2010(2):25-28.)

[8]李文治,杨连生.大豆低聚糖研究进展[J].粮食与油脂,2002(7):50-52.(Li W Z,Yang L S.Progress in study on soybean oligosaccharides[J].Cereals & Oils,2002(7):50-52.)

[9]李华,赵振贵,李丹,等.大孔树脂对大豆异黄酮的吸附性能研究[J].郑州大学学报,2011,32(2):19-22.(Li H,Zhao Z G,Li D,et al.Adsorption properties of macroporous resin for soybean isoflavones[J].Journal of Zhengzhou University,2011,32(2):19-22.)

[10]邢志田,申晓芳,郭峰.大孔树脂法提取大豆废料中金雀异黄素的研究[J].科技信息,2011(17):473-475.(Xing Z T,Shen X F,Guo F.Study on the extracting genistein from the soybean waste by the method of macroporous resin[J].Science & Technology Information,2011(17):473-475.)

[11]许浮萍,梁志家,田娟娟.应用膜分离结合醇沉法纯化大豆异黄酮[J].食品科学，2009,30(16):78-82.(Xu F P,Liang Z J,Tian J J.Use of membrane separation combined with alcohol precipitation for the separation and purification of isoflavones from lowdenatured soybean meal[J].Food Science,2009,30(16):78-82.)

[12]于殿宇,关海君,周凤超，等.利用膜技术及醇沉法纯化大豆异黄酮的研究[J].食品工业,2008(1):21-22.(Yu D Y,Guan H J,Zhou F C,et al.Study on the sublimating soybean isoflavone by the method of membrane technic and ethanol deposit[J].The Food Industry,2008(1):2l-22.)

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备注/Memo

基金项目：浙江省面向社会发展科技计划项目（2009C33031）。

第一作者简介：方建（1987-），男，在读硕士，研究方向为中药质量控制技术。E-mail:yamaxun2006@163.com。
通讯作者：颜继忠(1964-)，男，教授，主要从事中药提取技术与质量控制研究。E-mail:yjz@zjut.edu.cn。

更新日期/Last Update: 2014-08-17