Chun-bin,ZHANG Wei,LIU Biao.Effects of Transgenic Soybean Feed on Proliferation of Spleen Lymphocyte in Male Mice[J].Soybean Science,2012,31(02):291-294.[doi:10.3969/j.issn.1000-9841.2012.02.028]
抗草甘膦转基因大豆饲料对雄性小鼠脾淋巴细胞体外增殖的影响
- Title:
- Effects of Transgenic Soybean Feed on Proliferation of Spleen Lymphocyte in Male Mice
- 文章编号:
- 1000-9841（2012）02-0291-04
- 分类号:
- S816.7
- 文献标志码:
- A
- 摘要:
- 以雄性昆明鼠为动物模型，对亲本（F0）和子一代（F1）短期（30 d）及长期（90 d）喂食抗草甘膦转基因大豆饲料后，取其脾脏，MTT法检测脾淋巴细胞增殖情况。结果表明：短期喂食实验过程中，在0、2、7、14和30 d取样时，转基因大豆饲料对亲本（F0）实验的雄性小鼠脾脏淋巴细胞体外增殖均无显著性影响（P＞0.05），在长期（90 d）喂食实验过程中，抗草甘膦转基因大豆饲料对亲本（F0）小鼠脾脏淋巴细胞体外增殖也无显著影响（P＞0.05）。同样，在30 d的短期和90 d的长期喂食实验过程中，抗草甘膦转基因大豆饲料对子一代（F1）雄性小鼠脾脏淋巴细胞体外增殖也均无显著抑制作用（P＞0.05）。表明短期（30 d）及长期（90 d）喂食含抗草甘膦转基因大豆饲料对亲本及子一代雄性小鼠脾脏淋巴细胞体外增殖均无显著性影响，且无遗传积累效应。
- Abstract:
- In recent years,there have been a notable concerns on the safety of genetically modified(GM)foods,especially transgenic soybean.To evaluate effects of soybean on immunological function and possible genetic effects for progeny in male mice，male Kunming mice were fed with glyphosate feed for short-term of 30 and long-term of 90 days.Cell proliferation of spleen lymphocyte was analyzed with MTT assay.The sampling time was 0,2,7,14 and 30 day.For progeny test,male mice and female fed with transgenic soybean feed were mated and produced progeny F1.There were no significant adverse effects on cell proliferation of spleen for parent mice at different time during 30 day feeding trial（P＞0.05）.It was also showed that cell proliferation of spleen was not affected for long-term of 90 days.For progeny,no adverse effect on cell proliferation of spleen was found both for 30 and 90 days.It was showed that there was no significant adverse effect on cell proliferation of spleen for parent and progeny male mice during 30 and 90 day feeding trial of transgenic soybean.There was no possible potential of genetic toxicology in male mice fed with transgenic soybean.
参考文献/References:
[1]林清,彭于发,吴红,等.转基因作物及产品检测技术研究进展[J].西南农业学报,2009,22(2):513-518.(Lin Q,Peng Y F,Wu H,et al.Research progress of detection techniques for genetically modified crops and products[J].Southwest China Journal of Agricultural Sciences,2009,22(2):513-518.) [2]Richard E G,Afua O T.Suggested improvements for the allergenicity assessment of genetically modified plants used in foods[J].Current Allergy and Asthma Reports,2011,11(4):317-324. [3]Hammond B G,Dudek R,Lemen J K,et al.Results of a 90-day safety assurance study with rats fed grain from corn borer-protected corn[J].Food and Chemical Toxicology,2006,44(7):1092-1099. [4]Susan A M,Ian L,Jean S,et al.Thirteen week feeding study with transgenic maize grain containing event DAS-?15?7-1 in Sprague-Dawley rats[J].Food and Chemical Toxicology,2007,45(4):551-562. [5]谭建专,刘莎莎,孙哲,等.抗草甘膦转基因豆粕对肉仔鸡肠粘膜免疫的影响[J].动物营养学报,2011,23(5):836-841.(Tan J Z,Liu S S,Sun Z,et al.Effects of Glyphosate-tolerant soybean meal on intestinal mucosal immunity in broilers[J].Chinese Journal of Animal Nutrition,2011,23(5):836-841.) [6]李玮,蒋莹,楚建平,等.地黄水提液对BALB/c雄性小鼠脾细胞体外增殖作用的研究[J].中国煤炭工业医学杂志,2010,13(7):1409-1451.(Li W,Jiang Y,Chu J P,et al.Study on proliferation of spleen cells in BALB/C mice stimulated by Rehmannia glutinosa[J].Chinese Journal of Coal Industry Medicine,2010,13(7):1409-1451.) [7]胡庭俊,康乐,帅学宏,等.山豆根多糖对雄性小鼠脾脏淋巴细胞体外增殖及分泌一氧化氮的影响[J].中药药理与临床,2009,25(5):70-72.(Hu T J,Kang L,Shuai X H,et al.The effect of?Sophora subprosrate?polysaccharide on proliferation and nitric oxide release of splenic lymphocytes from mice[J].Pharmacology and Clinics of Chinese Materia Medica,2009,25(5):70-72.) [8]刘民,马华,李柏青.MTT法检测雄性小鼠淋巴细胞增殖性反应探讨[J].中国实验动物学杂志,1999,9(3):146-149.(Liu M,Ma H,Li B Q.Investigation of detecting murine lymphocyte proliferate response by MTT assay[J].Chinese Journal of Laboratory Animal Science,1999,9(3):146-149.) [9]JoséL D,Jordi G B.A literature review on the safety assessment of genetically modified plants[J].Environment International,2011,37(4):734-742. [10]Liu S,Mizu H,Yamauchi H.Molecular response to phototoxic stress of UVB-irradiated ketoprofen through arresting cell cycle in G2/M phase and inducing apoptosis[J].Biochemical and Biophysical Research Communications,2007,364(3):650-655. [11]Megumi K,Hiroaki S,Yuko K,et al.CR/periphilin is a transcriptional co-repressor involved in cell cycle progression[J].Biochemical and Biophysical Research Communications,2007,364(4):930-936.
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备注/Memo
基金项目：农业部转基因生物新品种培育重大科技项目（2011ZX08012-005）；广东省科技计划项目 (2010B031600108）。