WU Li-fang,LAI Hong-e,YANG Hua,et al.Effects of Soybean Glycinin on the Health of Fishes[J].Soybean Science,2012,31(01):145-148.[doi:10.3969/j.issn.1000-9841.2012.01.033]
大豆抗原蛋白Glycinin及其对鱼类健康的影响
- Title:
- Effects of Soybean Glycinin on the Health of Fishes
- 文章编号:
- 1000-9841（2012）01-0145-04
- Keywords:
- glycinin; immunoreactivity; anti-nutritional role; fishes
- 分类号:
- S816.4
- 文献标志码:
- A
- 摘要:
- 大豆抗原蛋白既是大豆的主要营养成分，又是大豆抗营养因子的主要种类之一，是限制大豆蛋白源在食品及饲料行业广泛应用的关键因素。其中，大豆球蛋白（Glycinin）和β-伴大豆球蛋白（β-Conglycinin）是免疫原性最强的大豆蛋白。Glycinin具有较强的热稳定性，普通的热处理对其免疫原性的灭活能力较小。Glycinin在鱼类饲料中含量过高，会引起鱼类肠道损伤，消化酶活力降低，生长缓慢，甚至死亡。文中简要介绍了大豆抗原蛋白Glycinin的理化性质、识别检测方法及对鱼类健康的影响，并对该领域的研究方向进行了探讨与展望,旨在为大豆抗原蛋白的加工去除及合理开发利用大豆蛋白源提供依据。
- Abstract:
- Soybean allergenic proteins,the main nutrient component,are identified as anti-nutritional factors in soybean,which limits their utilization in food and feed industries.Glycinin and β-Conglycinin,as two major allergens,have the highest immunoreactivity among all kinds of soybean anti-nutritional factors.Heating process has little effects on the immunoreactivity of Glycinin due to its strong heating stability.Too much Glycinin in feed lead to injured intestinal tissue,decreased activity of digestive enzyme,slow growth,and even death.The physicochemical property,detective methods of Glycinin,effects on healthy of fishes,and research prospect were reviewed,providing a basis for the inactive processing and reasonable exploitation of soybean proteins.
参考文献/References:
[1]Tsukada Y,Kitamura K,Harada K,et al.Geneticanalysis of two major subunits(β-Conglycinin and glycinin)in soybean seeds[J].Japanese Journal of Breeding，1986,36:390-400. [2]Montagne L,Toullec R,Savidge T C,et al.Influence of protein source and antigenicity of soyabean on morphology and enzyme activities of the proximal jejunum in preruminant calf[J].Reproduction Nutrition Development,1999,39:80-81. [3]孙泽威,秦贵信,张庆华.大豆抗原蛋白对犊牛生长性能、日粮养分消化率和肠道吸收能力的影响[J].中国畜牧杂志,2005,41(11):30-33.(Sun Z W,Qin G X,Zhang Q H.Effects of soybean protein antigen to the growth performance,diet digestibility,intestinal absorption of calf[J].Chinese Journal of Animal Science,2005,41(11):30-33.) [4]Mujoo R,Trinh D T,Ng P K W.Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture[J].Food Chemistry,2003,82:265-273. [5]李德发.大豆抗营养因子[M].北京：中国科学技术出版社，2003.(Li D F.Soybean anti-nutritional factors[M].Beijing:Chinese Science and Technology Press，2003.) [6]Li D F,Nelssen J L,Reddy P G,et al.Transient hypersensitivity to soybean meal in the early-weaned pig[J].Journal of Animal Science,1990,68:1790-1799. [7]Seegraber F J,Morrill J L.Effect of protein source in calf milk replacers on morphology and absorptive ability of the small intestine[J].Journal of Dairy Science,1986,69(2):460-469. [8]Christensen H R,Susanne W B,Frokiaer H.Antigenic specificity of serum antibodies in mice fed soy protein[J].International Archives of Allergy and Applied Immunology,2003,132(1):58-67. [9]Johnston C.Effect of injecting lambs with soyflour extract on serum soy protein antibody concentration and rate of gain[J].Small Ruminant Research,1996,21(2):149-154. [10]Golubovic M,van Hateren S H,Ottens M,et al.Novel method for the production of pure glycinin from soybeans[J].Agricultural and Food Chemistry,2005,53:5265-5269. [11]Wolf W J,Nelsen T C.Partial purification and characterization of the 15S globulin of soybeans,a dimmer of glycinin[J].Journal Agricultral and Food Chemistry,1996,44:785-791. [12]朱丹.大豆主要抗原蛋白在鲤鱼肠道组织中分布规律的研究[D].长春:吉林农业大学,2008.(Zhu D.Research advance in study on allergen proteins in soybean[D].Changchun:Jilin Agricultural University,2008.) [13]王洪鹤.大豆主要抗原蛋白对埃及胡子鲇肌肉营养成分的影响[J].吉林农业大学学报2009,31(6):741-745.(Wang H H.Effects of main soybean antigens on nutritional components of muscle in Clarias leather[J]Journal of Jilin Agricultural University,2009,31(6):741-745.) [14]赵元.大豆球蛋白及β-伴大豆球蛋白在仔猪体内消化动力学的研究[D].长春：吉林农业大学，2006.(Zhao Y.A Study of digestion dynamics of Glycinin and β-conglycinin in piglets[D].Changchun:Jilin Agricultural University,2006.) [15]Koppclman S J,Hcflc S L.Detecting allergens in food[M].Cambridge:Woodhead Publishing Ltd CRC Press LLC,2006. [16]张胜正.食物过敏原致敏组分的分析[D].北京:中国协和医科大学,2004.(Zhang S Z.Analysis of allergic components in food allergens[D].Beijing：Peking Union Medical College,2004.) [17]吴莉芳,孙泽威,秦贵信,等.Glycinin和β-Conglycinin对不同食性鱼类生长及肠道组织的影响[J].西北农林科技大学学报(自然科学版),2011,39（2）:59-66,74.(Wu L F,Sun Z W,Qin G X,et al.Effects of dietary Glycinin and -conglycinin on growth performance and intestinal tissue of fishes with different food habits[J].Journal of Northwest A & F University(Natural Science Edition),2011,39（2）:59-66,74.) [18]Rumsey G L,Siwicki A K,Anderson D P et al.Effect of soybean protein on serological response,non-specific defense mechanisms,growth,and protein utilization in rainbow trout[J].Veterinary Immunology and Immunopatholgy,1994，41:323-339. [19]钱曦,王桂芹,周洪琪,等.饲料蛋白水平及豆粕替代鱼粉比例对翘嘴红鲌消化酶活力的影响[J].动物营养学报,2007,19(2):182-187.(Qian X,Wang G Q,Zhou H Q,et al.Effect of dietary protein on the activities of digestive enzymes of topmouth culter[J].Chinese Journal of Animal Nutrition,2007,19(2):182-187.) [20]吴莉芳,秦贵信,刘春力,等.饲料大豆蛋白对鲤鱼消化酶活力和血液主要生化指标的影响[J].西北农林科技大学学报（自然科学版）,2009,37（8）:63-69.(Wu L F,Qin G X,Liu C L, et al.Effect s of dietary soybean protein on the activity of digestive enzyme and blood biochemical parameters of carp[J].Journal of Northwest A & F University(Natural Science Edition）,2009,37（8）:63-69.) [21]孙玲.大豆抗原蛋白对不同食性鱼类消化酶活性及血液指标的影响[D].长春:吉林农业大学,2008.(Sun L.Effects of soybean antigen on the activities of digestive enzyme and blood index of different fishes[D].Changchun:Jilin Agricultural University,2008.) [22] Dabrowski K,Kozak B.The use of fish meal and soybean meal as a protein source in the diet of grass carp fry[J].Aquaculture,1979,18:107-144. [23] Chou R L,Her B Y,Su M S,et al.Substituting fish meal with soybean meal in diets of juvenile cobia Rachycentron canadum[J].Aquaculture,2004,229:325-333. [24]张锦秀,周小秋,刘扬.去皮豆粕对幼建鲤生长性能和肠道的影响[J].中国水产科学,2007,14（2）:315-319.(Zhang J X,Zhou X Q,Liu Y.Effects of dehulled soybean meal on growth performance and intestine of juvenile Cyprinus carpio?var.jian[J].Journal of Fishery Sciences of China,2007,14（2）:315-319.)
备注/Memo
基金项目：吉林省自然科学基金资助项目(20101577)；吉林省教育厅资助项目（2009360）。