LIU Jiao,TIAN Yi-long,WANG Yi-hong,et al.Effects of Semen Sojae Preparatum Extract on the Expression of iNOS and eNOS mRNA in Aorta of Rats with Type 2 Diabetes Mellitus[J].Soybean Science,2012,31(01):115-118.[doi:10.3969/j.issn.1000-9841.2012.01.026]
淡豆豉提取物对2型糖尿病大鼠主动脉iNOS,eNOS mRNA表达的影响
- Title:
- Effects of Semen Sojae Preparatum Extract on the Expression of iNOS and eNOS mRNA in Aorta of Rats with Type 2 Diabetes Mellitus
- 文章编号:
- 1000-9841(2012)01-0115-04
- Keywords:
- SSP extract; T2DM; Large-vessel disease; NOS
- 分类号:
- R285.5
- 文献标志码:
- A
- 摘要:
- 为观察淡豆豉提取物对NO/NOS系统的影响,探讨其对2型糖尿病大鼠大血管保护作用的机制。采用高糖高脂饲料喂养加小剂量链尿佐菌素腹腔注射的方法复制2型糖尿病大鼠模型,给药56 d后检测大鼠空腹血糖(FBG)、血清胰岛素(FIN),计算胰岛素敏感指数(ISI),放免法检测血清肿瘤坏死因子-α(TNF-α)及一氧化氮(NO)水平,RT-PCR法检测主动脉内皮型一氧化氮合酶(eNOS)和诱导型一氧化氮合酶(iNOS)mRNA表达。结果显示:淡豆豉提取物能显著降低T2DM大鼠FBG、FIN、TNF-α、NO水平,显著提高ISI,显著降低iNOS mRNA表达,对eNOS mRNA表达则无明显影响。淡豆豉提取物可影响2型糖尿病大鼠NO/NOS系统,并可能通过此途径对2型糖尿病大鼠大血管产生保护作用。
- Abstract:
- To study the effect of Semen Sojae Praeparatum(SSP)extract on the NO/NOS system in T2DM rats.A T2DM rat model was developed by means of high-sugar,high-fat feed and raperitoneal injection of low-dose streptozotocin;after 56 days drug administration,the levels of fasting blood-glucose(FBG)and fasting serum insulin(FIN)in the rats were detected,insulin sensitivity index(ISI)was calculated;serum tumor necrosis factor-α(TNF-α)and nitric oxide(NO)level were detected by radioimmunoassay.And the expression of aortic endothelial nitric oxide synthase(eNOS)and inducible nitric oxide synthase(iNOS)mRNA was detected by RT-PCR.The levels of FBG,FIN,TNF-α and NO in T2DM rats were decreased lowered substantially,ISI was increased significantly,iNOS mRNA expression was markedly reduced,and eNOS mRNA expression was not obviously affected by SSP extract.These results suggest that SSP extract can affect the expression of iNOS mRNA and the level of NO in the T2DM rats,and may play a role in the protection against macrovascular disease of large vessels in T2DM rats.
参考文献/References:
[1]牛丽颖,常淑凤,刘姣,等.淡豆豉正丁醇提取物对糖尿病大鼠血糖及糖耐量的影响[J],时珍国医国药,2008,19(6):1398-1399.(Niu L Y,Chang S F,Liu J,et al.Effect of n-butanol extracts from Semen Sojae Praeparatum on blood glucose and glucose tolerance in diabetic rats[J].Lishizhen Medicine and Materia Medica Research,2008,19(6):1398-1399.) [2]白霞,牛丽颖,刘娇,等.淡豆豉防治早期动脉粥样硬化大鼠血管损伤的机制研究[J].时珍国医国药,2008,19(1):170-171.(Bai X,Niu L Y,Liu J,et al.Effect and mechanism of Semen Sojae Praeparatum on vascular injury in early atheroscherosis rats[J].Lishizhen Medicine and Materia Medica Research,2008,19(1):170-171.)〖HJ〗 [3]牛丽颖,刘娇,崔力剑,等.淡豆豉对早期动脉粥样硬化大鼠血管内皮损伤的保护作用[J].中药药理与临床,2007,23(5):120-122.(Niu L Y,Liu J,Cui L J,et al.The protective effect of Semen Sojae Praeparatum on vascular injury in early atheroscherosis rats[J].Pharmacology and Clinics of Chinese Material Medica,2007,23(5):120-122.) [4]Kanbak G,Akalin A,Dokumacioglu A,et al.Cardiovascular risk assessment in patients with type 2 diabetes mellitus and metabolic syndrome:role of biomarkers[J].Diabetes &Metabolic Syndrome:Clinical Research &Reviews,2011,5:7-11. [5]Makino N,Maeda T,Sugano M,et al.High serum TNF-αlevel in type 2 diabetic patients with microangiopathy is associated with eNOS down-regulation and apoptosis in endothelial cells[J].Journal of Diabetes and its Complication,2005,19:347-355. [6]Kobayashi T,Kamata K.Effect of chronic insulin treatment on NO production and endothelium-dependent relaxation in aortae from established STZ-induced diabetic rats[J].Atherosclerosis,2001,155:313-320. [7]赵怀兵,党瑜华.诱导型一氧化氮合酶对大鼠心肌梗死后心室重构的影响[J].中国临床康复,2006,10(16):46-48.(Zhao H B,Dang Y H.Effect of inducible nitric oxide synthase on ventricular remodeling in rats after myocardial infarction[J].Chinese Journal of Clinical Rehabilitation,2006,10(16):46-48.) [8]何敏,徐济良,吴锋.2型糖尿病大鼠主动脉内皮细胞氧化损伤及缬沙坦的保护作用[J].中国药理学通报,2007,23(3):354-358.(He M,Xu J L,Wu F.Aortic endothelial cells injury induced byoxidative stress in type 2 diabetes rats and the protective effect of valsartan[J].Chinese Pharamacological Bulletin,2007,23(3):354-358.) [9]Malvshev E,Poirel O,Moreau R.Interaction between eNOS and iNOS in aortas and superior mesenteric arteries in portal hyperrensive rats[J].De Biologic Vasculaire,2003,38:63.
备注/Memo
基金项目:河北省自然科学基金资助项目(C2009001061);河北科学技术研究与发展计划项目(11231005D)。