LI Ya-jing,WU Jian-yang,ZHENG Ya-ting.Technology for Microwave-assistant Extraction of Soyasaponins from Bean Dregs and Its Effects on Tyosinase Activity[J].Soybean Science,2012,31(01):112-114,118.[doi:10.3969/j.issn.1000-9841.2012.01.025]
大豆皂苷的微波辅助提取工艺及对酪氨酸酶活性影响的研究
- Title:
- Technology for Microwave-assistant Extraction of Soyasaponins from Bean Dregs and Its Effects on Tyosinase Activity
- 文章编号:
- 1000-9841（2012）01-0112-03
- 分类号:
- TS214.2
- 文献标志码:
- A
- 摘要:
- 通过正交实验考察了微波提取温度、微波提取时间、料液比、提取次数对大豆皂苷得率的影响，并研究了大豆皂苷对酪氨酸酶活性的影响。结果表明：微波辅助提取大豆皂苷的较佳工艺为：微波处理时间30 min，微波处理温度40℃，料液比1∶5，提取3次。在上述条件下测得大豆皂苷得率8.11%；大豆皂苷对酪氨酸酶活性具有抑制作用，且随浓度的增加，抑制作用增强，其IC50值为222.7 μg·mL-1。
- Abstract:
- Microwave-assistant extraction technology was used to obtain soyasaponins from bean dregs and the optimum technology parameters were determined by orthogonal experiment. Tyrosinase activity was determined by the dopachrome method using L-DOPA as the substrate and the amount of dopachrome in the reaction mixture was measured by spectrophotometer.The optimum technological parameters for soyasaponins extraction were obtained as follow:microwave extraction temperature at 40℃，microwave extraction for 30 min，the solid-liquid ratio 1∶5 and extracted for 3 times.Under above conditions，the soyasaponins content in bean dregs was 8.11%.Soyasaponins had an appreciable antityrosinase activity and the inhibitory effect was enhenced with the concentration increasing，the value of half inhibitory concentration(IC50)was 222.7 μg·mL-1.
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相似文献/References:
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LIU Zhong-hua,ZHAO Jin-hui,LIANG Shao-jun.Microwave-assisted Extraction of Soybean Isoflavone from Soybean Meal[J].Soybean Science,2012,31(01):993.[doi:10.3969/j.issn.1000-9841.2012.06.029]
[2]吴素萍,田立强.大豆皂苷的生理功能及其提取纯化的研究现状[J].大豆科学,2008,27(05):883.[doi:10.11861/j.issn.1000-9841.2008.05.0883]
WU Su-ping,TIAN Li-qiang.Research Status Quo of Extraction and Purification and Physiological Functions of Soybean Saponin[J].Soybean Science,2008,27(01):883.[doi:10.11861/j.issn.1000-9841.2008.05.0883]
备注/Memo
基金项目：浙江省新苗人才计划（2010R441001）；浙江经贸职业技术学院创新项目（11CX03）。