[1]张倩,刘代成.野生大豆皂苷的提取与薄层色谱分析[J].大豆科学,2011,30(05):857-860.[doi:10.11861/j.issn.1000-9841.2011.05.0857]
　ZHANG Qian,LIU Dai-cheng.Extraction and Thin Layer Chromatography of Saponins from Wild Soybean[J].Soybean Science,2011,30(05):857-860.[doi:10.11861/j.issn.1000-9841.2011.05.0857]
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野生大豆皂苷的提取与薄层色谱分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年05期 页码: 857-860 栏目: 出版日期: 2011-10-25
Title:
Extraction and Thin Layer Chromatography of Saponins from Wild Soybean
文章编号:
1000-9841（2011）05-0857-04
作者:
张倩; 刘代成
山东师范大学生命科学学院，山东 济南 250014
Author(s):
ZHANG Qian; LIU Dai-cheng
College of Life Science,Shandong Normal University,Jinan 250014,Shandong,China
关键词:
野生大豆; 皂苷; 薄层色谱
Keywords:
Wild soybean; Saponins; Thin layer chromatography(TLC)
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.05.0857
文献标志码:
A
摘要:
皂苷具有多种药理作用，对野生大豆中各类皂苷的定量分析是研究野生大豆皂苷药理作用的基础。利用索氏提取器以甲醇热回流提取野生大豆中的皂苷，结合理化反应进行鉴别，以齐墩果酸作为对照品进行薄层层析，通过薄层扫描测定皂苷含量。结果表明：野生大豆皂苷1～5 μg范围内线性关系良好，平均回收率为98.98%，RSD为0.41%，薄层层析斑点清晰且分离良好，计算得总皂苷在野生大豆脱脂粕中的含量为1.91%，远高于在栽培大豆脱脂粕中的含量。该方法简便准确，结果稳定，可用于野生大豆皂苷的分析。
Abstract:
Saponins have many pharmacological actions．The determination of wild soybean saponins contents is the basis of the pharmacological experiments． In this experiment,wild soybean saponins were extracted by Soxhlet extractor with hot circulatory methanol,identified by physical-chemical reactions and the thin layer chromatography(TLC)with the oleanic acid taken as reference standard,and then the TLC scanner was used to determine the content of the saponins.The linear range of wild soybean saponins was from 1 to 5 μg;the average recovery was 98.98% with RSD of 0.41%.The TLC identification was distinct and the spots were clear.The content of saponins in defatted wild soybean was 1.91%,which was significantly higher than that in defatted cultivated soybean.The method is simple and accurate with stable outcomes,and suitable for analysis of wild soybean saponins.

参考文献/References:

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备注/Memo

第一作者简介：张倩（1987-），女，在读硕士，研究方向为水产品加工与贮藏工程。E-mail：zhangqian1115@163.com。

通讯作者：刘代成（1955-），男，教授，研究方向为动物性食品研究。E-mail：liudch@sdnu.edu.cn。

更新日期/Last Update: 2014-08-16