LI Dan,LIU Chun-lei,LIU Hao-Fei,et al.Response Surface Optimization of Soybean Production Technology of Bio-peptide[J].Soybean Science,2011,30(05):851-856.[doi:10.11861/j.issn.1000-9841.2011.05.0851]
响应面优化豆粕生物肽的生产工艺研究
- Title:
- Response Surface Optimization of Soybean Production Technology of Bio-peptide
- 文章编号:
- 1000-9841（2011）05-0851-06
- Keywords:
- Soybean meal; Bacillus subtilis; Fermentation; Response surface
- 分类号:
- TS201.1
- 文献标志码:
- A
- 摘要:
- 以高温豆粕为原料，枯草芽孢杆菌为试验菌株，在单因素试验基础上，以发酵液α-N含量为响应值，对摇瓶发酵制备大豆肽工艺条件进行响应面优化。确定最佳工艺条件为：豆粕粒度40目，初始pH 6.8，种子培养时间16.25 h，碳源用量1.75%，豆粕用量10.75%，接种量6.25%，发酵时间48 h。在此条件下，发酵液α-N含量为1 737 μg·mL-1。
- Abstract:
- Based on single factor experiment，the technology of preparing soybean peptide from shake-flask fermentation was optimized by response surface optimization method，taking α-N content in broth as response value，with high temperature soybean meal and Bacillus subtilis as material.The optimized conditions were:soybean meal size 40 mesh，initial pH 6.8，cultivation time 16.25 h，carbon amount 1.75%，soybean meal amount 10.75%，inoculation amount 6.25%，fermentation time 48 h.Under these conditions，the α-N content of broth was 1 737 μg·mL-1.
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备注/Memo
基金项目：宁德师范学院科研资助项目（2009-Y-035）；黑龙江省科技厅“十一五”重大攻关资助项目（GA06B402-08-4）。