DUAN Yu-xi,ZHANG Yu,ZHU Xiao-feng,et al.Biochemical Mechanism of Resistance against Soybean Cyst Nematode Induced by Plant Growth Promoting Rhizobacteria in Soybean[J].Soybean Science,2011,30(03):442-446.[doi:10.11861/j.issn.1000-9841.2011.03.0442]
根际促生菌诱导大豆抗大豆胞囊线虫的生化机理
- Title:
- Biochemical Mechanism of Resistance against Soybean Cyst Nematode Induced by Plant Growth Promoting Rhizobacteria in Soybean
- 文章编号:
- 1000-9841(2011)03-0442-05
- Keywords:
- PGPR; Induced resistance; Heterodera glycines; Defense enzymes; Chitinase; Total phenolics
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 为揭示由根际促生细菌[Sneb207(Bacillus megaterium)，Sneb482(Bacillus megaterium)]诱导大豆抗大豆胞囊线虫(Heterodera glycines Ichinohe)的生化机理。使用菌株Sneb207、Sneb482发酵液包衣处理大豆种子，在豆苗三叶期时接种大豆胞囊线虫卵悬液，分别于接种后6、12、18、24、30 d取样，测定大豆根内防御酶系活性（PAL，PPO，POD）、总酚含量和几丁质酶活性的动态变化。结果表明：大豆种子经Sneb207、Sneb482发酵液处理后，根内PAL、PPO、POD活性较对照均表现上升趋势，总酚含量也有所提高。与菌株Sneb482相比，菌株Sneb207表现出对大豆胞囊线虫病更好的诱导抗病潜力。
- Abstract:
- It has been a new research focus on biological control that the induction of disease resistance and growth response in plants is elicited by plant growth promoting rhizobacteria（PGPR）. This study aimed to examine the biochemical mechanism of the resistance to soybean cyst nematode(Heterodera glycines Ichinohe) in soybean induced by PGPR [Sneb207(Bacillus megaterium) and Sneb482(Bacillus megaterium)].Seed bacterization with Sneb207 and Sneb482 were utilized in the experiment. The soybean plants were inoculated with eggs of soybean cyst nematode after soybean trefoil stage and the samples of roots were obtained 6,12,18,24 and 30 days later. Activities of plant resistance correlated enzymes including defense enzymes, namely phenylalanine ammonia-lyase (PAL), polyphenoloxidase (PPO), peroxidase (POD) and chitinase were measured, the content of total phenolics was also determined. The results showed that both the activities of PAL, PPO, POD, chitinase and the contents of total phenolics could be increased significantly by seed coating with fermentation liquid of plant growth promoting rhizobacteria (Sneb207, Sneb482) in the soybean roots than those in control. Sneb207 showed greater potential in induced resistance against soybean cyst nematode in the soybean plants than Sneb482.
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备注/Memo
基金项目：国家现代农业产业技术体系资助项目；农业部公益性行业科技专项资助项目（20090340-03）。