[1]魏苗,李建东,燕雪飞,等.中国东北野生大豆SSR遗传多样性及亲缘关系分析[J].大豆科学,2011,30(03):388-392,396.[doi:10.11861/j.issn.1000-9841.2011.03.0388]
　WEI Miao,LI Jian-dong,YAN Xue-fei,et al.Analysis of Genetic Diversity and Relationship of Glycine soja in Northeast China[J].Soybean Science,2011,30(03):388-392,396.[doi:10.11861/j.issn.1000-9841.2011.03.0388]
点击复制

中国东北野生大豆SSR遗传多样性及亲缘关系分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年03期 页码: 388-392,396 栏目: 出版日期: 2011-06-25
Title:
Analysis of Genetic Diversity and Relationship of Glycine soja in Northeast China
文章编号:
1000-9841(2011)03-0388-05
作者:
魏苗1; 李建东1; 燕雪飞1; 王国骄1; 胡翼宁2; 田凤芝3
1. 沈阳农业大学 农学院，辽宁 沈阳 110866；
2. 北京麋鹿生态实验中心，北京 100076；
3. 彰武县兴隆堡乡农科农机站，辽宁 阜新 123200
Author(s):
WEI Miao1;  LI Jian-dong1;  YAN Xue-fei1;  WANG Guo-jiao1;  HU Yi-ning2; TIAN Feng-zhi3
1.College of Agronomy, Shenyang Agricultural University, Shenyang 110866,Liaoling;
2.Beijing Milu Ecological Experimental Center, Beijing100076;
3.Zhangwu County Xinglongbao Township Agricultural Machinery Station, Zhangwu County 123200,Liaoning,China
关键词:
野生大豆; 微卫星; 遗传多样性; 亲缘关系
Keywords:
Glycine soja;  SSR;  Genetic diversity;  Relationship
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0388
文献标志码:
A
摘要:
利用60对SSR引物对东北52份野生大豆资源进行遗传多样性分析。结果表明：①其中40对SSR引物扩增出408个等位变异，平均每个位点等位变异10.2个， Shannon-Weaver指数变化范围为1.2203~2.6392，平均为2.0560，东北野生大豆具有较丰富的遗传变异；②41~43°N×124~126°E区的野生大豆资源分布广、遗传多样性丰富，推测处于该经纬区间的辽河平原为东北野生大豆的初生遗传多样性中心；39~41°N×120~122°E区的野生大豆遗传多样性指数较高，但搜集的资源仅有4份，推测处于该经纬区间的辽东丘陵区可能为东北野生大豆的次生多样性中心。证明东北野生大豆形成了不均衡的2个多样性中心;③基于SSR数据，把52份野生大豆材料共聚为6大类，聚类结果与野生大豆材料地理分布具有一定的相关性。
Abstract:
The genetic diversity of 52 northeast wild soybeans were analyzed by 60 pairs of SSR primers.The results showed that: 408 alleles were detected by 40 pairs of SSR markers, with an average of 12.5 alleles for each locus. Shannon-Weaver diversity indexes varied from 1.2203 to 2.6392 with averaged 2.0560, wild soybean has a rich genetic variation in northeast China; wild soybean in 41~43°N×124~126°E area have the richest accessions and genetic diversity, thus the Liaohe plain might be the newborn genetic diversity centre. Wild soybean in 39~41 °N × 120~122 °E region have higher genetic diversity index, but fewer resources were collected, thus the Liaodong hilly area might be the secondary genetic diversity centre of the northeast China. It proved that the formation of two uneven wild soybean centers of diversity in northeast China. Based on SSR data, 52 wild soybeans were clustered into six categories, the results of clustering was related with the geographical distribution of wild soybeans.

参考文献/References:

[1]李福山. 大豆起源及其演化研究[J]. 大豆科学,1994,13(1):61-66. (Li F S. The study on origin and evolution of soybean[J]. Soybean Science, 1994,13(1):61-66.)

[2]庄炳昌. 中国野生大豆研究二十年[J]. 吉林农业科学,1999,24(5):3-10. (Zhuang B C. Researches on wild soybean (Glycine soja) in China for twenty years[J]. Journal of Jilin Agricultural Science,1999,24(5):3-10.)

[3]薛中天, 徐美琳, 庄乃亮, 等. 野生大豆(Glycine soja?SH1)球蛋白glycinin Gy4基因家族的两种表达拷贝[J]. 中国科学(B辑),1987(8):36-43. (Xuan Z T, Xu M L, Zhuang N L, et al. SH1 Two expression copies of glycinin Gy4?gene family in Glycine soja[J]. Science in China (B Series), 1987(8):36-43.)

[4]王果. 河南省野生大豆资源遗传多样性分析[D]. 杨凌:西北农林科技大学,2007. (Wang G. The genetic diversity of annual wild soybeans in Henan province[D]. Yangling: Northwest A & F University,2007.)

[5]赵丽梅, 董英山,刘宝,等.中国一年生野生大豆(Glycine soja)核心资源构建[J].科学通报, 2005(10):992-999. (Zhao L M, Dong Y S, Liu B, et al. Core resources construction of annual wild soybean (Glycine soja) in China[J]. Science Bulletin,2005(10):992-999.)

[6]李建东, 燕雪飞, 董思言, 等.辽宁省野生大豆种质资源的SSR遗传多样性分析[J]. 大豆科学, 2010,29(1):29-32. (Li J D, Yan X F, Dong S Y, et al. Analysis of genetic diversity ofGlycine soja?germplasm resources in Liaoning province[J]. Soybean Science,2010,29(1):29-32.)

[7]Doyle J J, Doyle J L. A rapid isolation procedure for small quantities of fresh leaf tissue[J]. Phytochemical Bulletin, 1987,9:11-15.

[8]Sanguinetti C J, Dias N E, Simpson A J G. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels[J]. Biotechniques,1994,17:915-919.

[9]Vavilov N I. The origin, variation, immunity and breeding of cultivated plants[M]. New York: Academic Press,1973.

[10]董英山, 庄炳昌, 赵丽梅，等.中国野生大豆遗传多样性中心[J]. 作物学报,2000,26(5):521-527. (Dong Y S, Zhuang B C, Zhao L M, et al. The genetic diversity centers of annual wild soybean in China[J]. Acta Agronomica Sinica,2000,26(5):521-527.)

[11]李福山, 王衍桐. 野生大豆分布与环境条件[J]. 大豆科学,1989,8(3):245-251. (LI F S, Wang Y T. The distribution and environment condition of wild soybean (Glycine soja)[J]. Soybean Science, 1989,8(3):245-251.)


相似文献/References:

[1]高越,刘辉,陶波.抗草甘膦野生大豆筛选及其抗性生理机制研究[J].大豆科学,2013,32(01):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
　GAO Yue,LIU Hui,TAO Bo.Screening and Physiological Mechanisms of Resistance to Glyphosate in Wild Soybeans(Glycine soja)[J].Soybean Science,2013,32(03):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
[2]王军卫,侯立江,李? 登,等.野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al.Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(03):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
[3]王军卫,侯立江,李 登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(03):596.
[4]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
　WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(03):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[5]陈丽丽,王明玖,何丽君,等.野生大豆ISSR体系的优化及其在远缘杂交后代鉴定中的利用[J].大豆科学,2013,32(04):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
　CHEN Li-li,WANG Ming-jiu,HE Li-jun,et al.Optimization for ISSR Reaction System of Wild Soybean and Its Utilization in Distant Hybrid Identification[J].Soybean Science,2013,32(03):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
[6]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
　ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(03):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[7]徐艳平,胡翠美,张文会,等.干旱胁迫对野生大豆幼苗光合作用相关指标的影响[J].大豆科学,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
　XU Yan-ping,HU Cui-mei,ZHANG Wen-hui,et al.Effect of Simulated Drought Stress on Photosynthesis Related Indexes at Seedling Stage of Wild Soybeans[J].Soybean Science,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
[8]胡卫静,何丽君,何劲莉,等.NaCl胁迫对野生与栽培大豆杂交后代株系生理指标的影响[J].大豆科学,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
　HU Wei-jing,HE Li-jun,HE Jin-li,et al.Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max × Glycine soja[J].Soybean Science,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
[9]王 旻,梁 玉,王欣欣,等.即墨野生大豆主要成分及其营养价值分析[J].大豆科学,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
　WANG Min,LIANG Yu,WANG Xin-xin,et al.Assessment on Nutritional Compositions and Value of Jimo Wild Soybean[J].Soybean Science,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
[10]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶（POD）活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
　CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(03):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]

备注/Memo

基金项目：辽宁省高等学校创新团队科研资助项目（2009T088）。

第一作者简介：魏苗（1987-），女，硕士，研究方向为野生大豆遗传多样性。E-mail:weimiao.1987@163.com。
通讯作者：李建东（1964-），男，教授，主要从事农业生态学和生物多样性研究。E-mail:sylijiandong@126.com。

更新日期/Last Update: 2014-09-12