[1]徐立恒,李向华.SSR标记对野生大豆种群遗传结构的研究[J].大豆科学,2011,30(01):41-45.[doi:10.11861/j.issn.1000-9841.2011.01.0041]
　XU Li-heng,LI Xiang-hua.Analysis on Genetic Structure of Wild Soybeans Populations by SSR Markers[J].Soybean Science,2011,30(01):41-45.[doi:10.11861/j.issn.1000-9841.2011.01.0041]
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SSR标记对野生大豆种群遗传结构的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年01期 页码: 41-45 栏目: 出版日期: 2011-02-25
Title:
Analysis on Genetic Structure of Wild Soybeans Populations by SSR Markers
文章编号:
1000-9841（2011）01-0041-05
作者:
徐立恒; 李向华
银川科技职业学院 生物与农业工程系，宁夏 银川 750105
Author(s):
XU Li-heng;  LI Xiang-hua
School of Biological and Agricultural Engineering, Yinchuan Science and Technology Vocational College, Yinchuan 750105, Ningxia, China
关键词:
野生大豆;  SSR标记;  遗传多样性
Keywords:
Wild soybean（Glycine soja）;  SSR marker;  Genetic diversity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.01.0041
文献标志码:
A
摘要:
使用 40对SSR引物分析了8个省区天然野生大豆种群的遗传结构和遗传多样性。结果表明：15个种群共检测到633个等位基因，平均每对引物等位变异基因数平均值15.83个；种群平均Shannon指数（I）0.7835，种群平均期望杂合度（He）0.4070，种群平均观察杂合度（Ho）0.0012，平均种群内遗传多样度（Hs）0.4277，平均种群间遗传多样度（DST）0.4543，种群分化系数(GST)0.5151；供试的15个天然野生大豆种群可分为4组，基本存在于2个地理分布区，天津以北地理区遗传多样性较高。
Abstract:
Wild soybean (Glycine soja) is commonly accepted as the progenitor species of the cultivated soybean (Giycine max).They contain many characters potentially valuable for supplementing the soybean germplasm pool. Our objective was to evaluate the genetic diversities of wild soybean collected from 8 provinces in different latitudes of China. We sampled 15 representative natural populations in 2005. Forty public SSR primer pairs over the 20 linkage groups were applied to evaluate genetic diversity. A total of 633 bands were detected with an average number of 15.83 per locus. Mean expected heterozygosity per locus (He) was 0.4070 for the populations, and the mean Shan-non index (I) for the population was 0.7835. Mean observed heterozygosity per locus (Ho) for the populations was 0.0012. Between-population genetic diversity (Hs) averaged 0.4277, and within-population genetic diversity (DST) averaged 0.4543. Mean coefficient of gene differentiation for loci (GST) in the populations was estimated to be 0.5151. Cluster analysis showed that 15 populations fell into 4 groups, and existed mainly in geography distribution area, and the area with the latitude higher than of Tianjin had better genetic diversity.

参考文献/References:

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备注/Memo

第一作者简介：徐立恒（1980-），女，硕士，主要从事分子生物学研究。E-mail:42598919@qq.com。

更新日期/Last Update: 2014-09-11