LI Jian-ying,ZHOU Chang-jun,YANG Liu,et al.Effect of Water Stress on Endogenous Hormone and Protective Enzymes in Soybean Seedling Leaves[J].Soybean Science,2010,29(06):959-963.[doi:10.11861/j.issn.1000-9841.2010.06.0959]
水分胁迫对大豆苗期叶片内源激素含量与保护酶活性的影响
- Title:
- Effect of Water Stress on Endogenous Hormone and Protective Enzymes in Soybean Seedling Leaves
- 文章编号:
- 1000-9841(2010)06-0959-05
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 在盆栽条件下,以大豆(Glycine max)绥农10号为材料,通过人为控制水分(干旱和水渍处理),比较叶片中几种内源激素含量变化的差异,研究水分胁迫对大豆幼苗保护酶活性的影响。结果表明:干旱处理提高了大豆苗期叶片内赤霉素(GA)、脱落酸(ABA)的含量但也降低了玉米素核苷(ZR)和生长素(IAA)的含量,同时干旱处理提高了丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性;水渍处理降低了ABA含量和SOD活性,提高了IAA、GA以及MDA的含量。
- Abstract:
- “Suinong10”, a soybean (Glycine max) cultivar, was used as materials in this study. Drought and water-logged treatments were employed to compare differences of several endogenous hormones and protective enzyme activities in soybean seedling leaves. The results showed that the content of GA and ABA were increased, the content of ZR and IAA were reduced with the drought treatment, the content of MDA and the activity of SOD was increased. Moreover, the content of IAA, GA and MDA were increased under the water stress, howevwer, the content of ABA and the activity of SOD were decreased in soybean seedling leaves.
参考文献/References:
[1]李玉梅, 李建英, 王根林, 等. 水分胁迫对大豆幼苗叶片内源激素的影响[J]. 大豆科学, 2007, 26(4): 627-629,636. (Li Y M, Li J Y, Wang G L, et al. Studies on mechanism of endogenous hormones in soybean seedling under water stress[J].Soybean Science, 2007, 26(4): 627-629,636.) [2]陈少裕,刘杰. 干旱胁迫对甘蔗叶片线粒体膜流动性的影响及其与膜脂过氧化的关系[J]. 植物生理学报, 1991,17(3):285-289. (Chen S Y, Liu J. The effect of water stress on membrane fluidity of leaf mitochondria of sugarcane and its relation to membrane lipid peroxidation[J]. Acta Phytophysiologica Sinica, 1991, 17(3):285-289.) [3]张敬贤,李俊明,崔四平,等. 玉米细胞保护酶活性对苗期干旱的反应[J]. 华北农学报,1990,5(增刊):19-23. (Zhang J X, Li J M, Cui S P, et al. Responses of protecting enzymes activity of maize cell to the drought of seedling[J]. Acta Agriculturae Boreali-Sinica, 1990,5(supplementary issue):19-23.) [4]Pavenport T L, Morgan P W, Jordan W R. Reduction of auxin transport capacity with age and internal water deficits in cotton petioles[J]. Plant Physiology, 1980, 65: 1023-1025. [5]Blackman P G, Davies W J. Cytokinins, Abscisic acid and the control of plant water balance[J]. Acta Horticulturae, 1985, 171: 255-261. [6]Masia A, Pitacco A, Braggil L, et al. Hormonal responses to partial drying of the root system of Helianthus annuns[J]. Journal of Experimental Botany, 1994, 45: 69-76 [7]何钟佩.农作物化学控制实验指导[M]. 北京:中国农业出版社,1993:60-68.(He Z P. Experimental directions of crop chemical contral[M].Beijing: China Agriculture Press,1993:60-68.) [8]张宪政.作物生理研究法[M].北京: 农业出版社,1990. (Zhang X Z. Crop physiology research methods [M]. Beijing: Agriculture Press,1990.) [9]邹琦.植物生理生化实验指导[M].北京:中国农业出版社,1995.(Zou Q. The guidance of plant physiological and biochemical[M].Beijing: China Agriculture Press,1995.) [10]郑殿峰, 赵黎明,冯乃杰.植物生长调节剂对大豆叶片内源激素含量及保护酶活性的影响[J].作物学报, 2008, 34(7): 1233-1239. (Zheng D F, Zhao L M, Feng N J. Effects of PGRs on endogenous hormone contents and activities of protective enzymes in soybean leaves[J].Acta Agronomica Sinica,2008,34(7):1233-1239.) [11]潘瑞炽, 李玲. 植物生长发育的化学控制[M]. 广州: 广东高等教育出版社,1999.(Pan R C,Li L.The chemical control of plant growth and development[M].Guangzhou:Guangdong Highter Education Press,1999.) [12]Daie J, Compbell W F. Response of tomato plants to stressful temperatures[J].Plant Physiology, 1981, 67:26-29. [13]马原松,王启明,吴诗光,等.干旱胁迫下大豆苗期生理生化指标的研究[J].安徽农业科学,2005,33(6): 974-976. (Ma Y S, Wang Q M, Wu S G, et al. Research on the physiological-biochemical indexes of different soybean varieties during seedling period under the drought stress [J]. Journal of Anhui Agricultural Sciences, 2005, 33(6): 974-976.) [14]施晓明,李淑芹,许景钢,等.干旱胁迫下DA-6浸种对大豆苗期叶片保护酶活性的影响[J].东北农业大学学报, 2009, 40(9): 48-51. (Shi X M, Li S Q, Xu J G, et al. Effect of soaking the seeds in DA-6 on protective enzyme activities in leaves of soybean seedling under drought stress [J]. Journal of Northeast Agricultural University, 2009, 40(9): 48-51.) [15]汪耀富, 韩锦峰, 林学梧. 烤烟生长前期对干旱胁迫的生理生化响应研究[J].作物学报,1996, 22(1): 117-121. (Wang Y F, Han J F, Lin X W. Study on physiological and biochemical responses of flue-cured tobacco to drought stress during early growth of the plants[J].Acta Agronomica Sinica, 1996,22(1):117-121.) [16]樊金娟, 李雪梅, 阮燕晔, 等. 杂交水稻及其灌浆过程中内源激素含量的变化[J]. 植物生理学通讯, 2004, 40(2): 146-148. (Fan J J, Li X M, Ruan Y Y, et al. Changes in endogenous hormone contents during grain filling in hybrid rice and its parents [J].Plant Physiology Communications, 2004, 40(2):146-148.) [17]Blatt M R, Grehov A. Signaling gates in abscisic acid-mediated control of guard cell ion channels[J]. Physiology Plant, 1997,100: 481-490. [18]Liang J, zhang J, Wong M H. Can stomatal closure caused by xylem ABA explain the inhibition of leaf photosynthesis undersoil drying ? [J]. Photosynthesis Research, 1997,51:149-159. [19]邱全胜, 李琳, 梁厚果, 等. 水分胁迫对小麦根细胞质膜氧化还原系统的影响[J].植物生理学报,1994,20(2):145-151.(Qiu Q S, Li L, Liang H G, et al. Effect of water stress on the redox reaction system of the plasma membrane of wheat roots[J].Acta Phytophysiologia Sinica,1994,20(2):145-151.) [20]李岩, 潘海春, 李德全.土壤干旱条件下玉米叶片内源激素含量及光合作用的变化[J].植物生理学报, 2000, 26(4): 301-305. (Li Y, Pan H C, Li D Q. Changes in contents of endogenous phytohormones and photosynthesis in leaves of maize in drying soil[J].Acta Phytophysiologia Sinica, 2000, 26(4): 301-305.) [21]Davies W J, Zhang J. Root signals and the regulation of growth and development of plants in drying soil[J]. Annual Reviewes on Plant Physiology and Plant Molecular Biology, 1991, 42:55-76. [22]莫红, 翟兴礼.干旱胁迫对大豆苗期生理生化特性的影响[J].湖北农业科学,2007, 46(1): 45-48.(Mo H, Zhai X L. Effects of drought stress on protective enzymes activities and membrane lipid peroxidation in leaves of soybean seedlings[J]. Hubei Agricultural Sciences, 2007, 46(1): 45-48.) [23]董钻, 谢甫绨. 土壤干旱胁迫对大豆体内酶活性和膜透性的影响[J].大豆科学, 1995, 14(4): 290-297. (Dong Z, Xie F T. Effects of drought treatments at different stages on the enzyme activities and membrane permeabilities in soybeans[J].Soybean Science, 1995,14(4): 290-297.) [24]于健, 陈全家, 李波, 等. 干旱处理对不同棉花材料SOD、CAT、MDA的影响[J]. 新疆农业大学学报, 2008, 31(5): 31-35. (Yu J, Chen Q J, Li B, et al. Influence of drought stress on three physiological and biochemical indexes of different cotton varieties [J].Journal of Xinjiang Agricultural University,2008, 31(5):31-35.) [25]陈少裕.膜脂过氧化对植物细胞的伤害[J].植物生理学通讯,1991, 27(2): 84-90. (Chen S Y. Injury of membrane lipid peroxidation[J]. Plant Physiology Communications,1991,27(2):84-90.) [26]严美玲,李向东,王丽丽,等.花生苗期不同程度干旱胁迫对叶片某些酶活性的影响[J].中国油料作物学报,2006,28(4):440-443. (Yan M L, Li X D, Wang L L, et al. Effects of different drought stress during seeding stage on some enzyme activities in peanut leaves[J].China Journal of Oil Crop Sciences,2006,28(4):440-443.)
相似文献/References:
[1]郝建军,黄春花,卢 环,等.K+、Na+和HCO-3对大豆幼苗光合作用的影响[J].大豆科学,2012,31(03):436.[doi:10.3969/j.issn.1000-9841.2012.03.020]
HAO Jian-jun,HUANG Chun-hua,LU Huan,et al.Influence of K+、Na+and HCO-3 on Photosynthesis of Soybean Seedlings[J].Soybean Science,2012,31(06):436.[doi:10.3969/j.issn.1000-9841.2012.03.020]
[2]郝建军,卢环,黄春花,等.HSO3-、Cl-和Na+对大豆幼苗光合作用的影响[J].大豆科学,2012,31(05):855.[doi:10.3969/j.issn.1000-9841.2012.05.037]
HAO Jian-jun,LU Huan,HUANG Chun-hua,et al.Influence of HSO3-、Cl-and Na+on Photosynthesis of Soybean Seedlings[J].Soybean Science,2012,31(06):855.[doi:10.3969/j.issn.1000-9841.2012.05.037]
[3]闫春娟,王文斌,孙旭刚,等.干旱胁迫对大豆根系发育影响初报[J].大豆科学,2012,31(06):920.[doi:10.3969/j.issn.1000-9841.2012.06.014]
YAN Chun-juan,WANG Wen-bin,SUN Xu-gang,et al.Effect of Drought Stress at Different Growth Stages on Root Development of Soybean[J].Soybean Science,2012,31(06):920.[doi:10.3969/j.issn.1000-9841.2012.06.014]
[4]常云霞,徐克东,陈 璨,等.水杨酸对低温胁迫下大豆幼苗生长抑制的缓解效应[J].大豆科学,2012,31(06):927.[doi:10.3969/j.issn.1000-9841.2012.06.015]
CHANG Yun-xia,XU Ke-dong,CHEN Can,et al.Salicylic Acid Mitigating the Inhibition of Low Temperature Stress to Soybean Seedlings[J].Soybean Science,2012,31(06):927.[doi:10.3969/j.issn.1000-9841.2012.06.015]
[5]梁 鹏,邢兴华,周 琴,等.α-萘乙酸对干旱和复水处理下大豆幼苗生长和光合作用的影响[J].大豆科学,2011,30(01):50.[doi:10.11861/j.issn.1000-9841.2011.01.0050]
LIANG Peng,XING Xing-hua,ZHOU Qin,et al.Effect of NAA on Growth and Photosynthetic Characteristic of Soybean Seedling under Drought and Re-watering[J].Soybean Science,2011,30(06):50.[doi:10.11861/j.issn.1000-9841.2011.01.0050]
[6]杨卫民,刘宝琦,张世珍.甘露醇、过氧化氢和氯化钠处理对黑豆苗期抗旱性的影响[J].大豆科学,2010,29(02):350.[doi:10.11861/j.issn.1000-9841.2010.02.0350]
YANG Wei-min,LIU Bao-qi,ZHANG Shi-zhen.Effect of Mannitol, Hydrogen Peroxide and Chloride Treatment on Drought Resistance of Black Soybean Seedling[J].Soybean Science,2010,29(06):350.[doi:10.11861/j.issn.1000-9841.2010.02.0350]
[7]周倩倩,杨维,梁婵娟,等.镧与酸雨对大豆幼苗叶绿素含量和光合速率的复合影响[J].大豆科学,2009,28(05):941.[doi:10.11861/j.issn.1000-9841.2009.05.0941]
ZHOU Qian-qian,YANG Wei,LIANG Chan-juan,et al.Effects of La and Acid Rain on Chlorophyll Content and Photosynthetic Rate of Soybean Seedling[J].Soybean Science,2009,28(06):941.[doi:10.11861/j.issn.1000-9841.2009.05.0941]
[8]范金凤,曾淑平,梁婵娟,等.La(Ⅲ)与酸雨对大豆幼苗生长的复合影响[J].大豆科学,2009,28(04):744.[doi:10.11861/j.issn.1000-9841.2009.04.0744]
FAN Jin-feng,ZENG Shu-ping,LIANG Chan-juan,et al.Effects of La(Ⅲ) and Acid Rain on Growth of Soybean Seedling[J].Soybean Science,2009,28(06):744.[doi:10.11861/j.issn.1000-9841.2009.04.0744]
[9]廖永霞,康玉凡,王保民,等.乙烯、6-BA对大豆幼苗生长、生化成分及细胞组织结构的效应[J].大豆科学,2009,28(01):41.[doi:10.11861/j.issn.1000-9841.2009.01.0041]
LIAO Yong-xia,KANG Yu-fan,WANG Bao-min,et al.Effects of Ethyle and 6-BA on Growth,Chemical Composition and Anatomical Structure of Soybean Seedling[J].Soybean Science,2009,28(06):41.[doi:10.11861/j.issn.1000-9841.2009.01.0041]
[10]白伟,孙占祥,刘晓晨,等.苗期水分胁迫对大豆器官平衡和产量的影响[J].大豆科学,2009,28(01):59.[doi:10.11861/j.issn.1000-9841.2009.01.0059]
BAI Wei,SUN Zhan-xiang,LIU Xiao-chen,et al.Effect of Water Stress at Seedling on Organ Equilibrium and Yield of Soybean[J].Soybean Science,2009,28(06):59.[doi:10.11861/j.issn.1000-9841.2009.01.0059]
[11]李玉梅,李建英,王根林,等.水分胁迫对大豆幼苗叶片内源激素的影响[J].大豆科学,2007,26(04):627.[doi:10.3969/j.issn.1000-9841.2007.04.034]
LI Yu-mei,LI Jian-ying,WANG Gen-lin,et al.STUDIES ON MECHANISM OF ENDOGENOUS HORMONES IN SOYBEAN SEEDLING UNDER WATER STRESS[J].Soybean Science,2007,26(06):627.[doi:10.3969/j.issn.1000-9841.2007.04.034]
备注/Memo
第一作者简介:李建英(1975-),女,硕士,助理研究员,主要从事大豆栽培育种研究。E-mail:lijianying617@126.com。