HUANG Guo-wei,WU Guang-hong,HE Jia-wen,et al.An Improved Electrophoresis Method for Determining Trypsin Inhibitors Activities and Variety from Bean[J].Soybean Science,2010,29(02):315-318.[doi:10.11861/j.issn.1000-9841.2010.02.0315]
测定豆类胰蛋白酶抑制剂活性和种类的电泳方法改良
- Title:
- An Improved Electrophoresis Method for Determining Trypsin Inhibitors Activities and Variety from Bean
- 文章编号:
- 1000-9841（2010）02-0315-04
- Keywords:
- Trypsin inhibitor; gelatin-PAGE; sensitivity
- 分类号:
- TS201.2
- 文献标志码:
- A
- 摘要:
- 以赤豆胰蛋白酶抑制剂（ABTI）及绿豆的胰蛋白酶抑制剂（MBTI）为材料，采用明胶-PAGE技术对其进行活性和种类检测。同时对影响明胶-PAGE技术体系的明胶浓度、酶解时间、脱酶液及电泳的灵敏度等因素进行探讨。结果表明：改良后的明胶-PAGE技术，鉴定胰蛋白酶抑制剂活性和种类的时间大大缩短，成本低，技术操作可行性强，重复性好，且检测ABTI的种类达7种，灵敏度达到每孔1.0 BAEE、蛋白点样量为每孔0.154 μg。
- Abstract:
- Trypsin inhibitor (TI), a major anti-nutritional factor, it’s variety and activities provided an important reference for the eradication of anti-nutritional factors in diet bean. In this study, we determined the activities and variety of Adzuki Bean Trypsin Inhibitor(ABTI) and Mung Bean Trypsin Inhibitor(MBTI) by means of gelatin-PAGE techniques; and explored the various factors of affecting the gelatin-PAGE, including gelatin concentration, enzyme hydrolysis time, the sector of rinse enzyme solution and the sensitivity of electrophoresis. The results showed that the improved gelatin-PAGE could detect 7 types of ABTI, its sensitivity reached 1.0 BAEE·hole-1 with the protein sampling volume of 0.154 μg·hole-1. The improved method had many advantages such as time and cost saving, easy to operate and reproducible.
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备注/Memo
基金项目：华南农业大学大学生科技创新资助项目（L06056）。