ZHANG Xiu-ling.Effects of Different Storage Period and Treatment on Seed Germination of Glycine Soja[J].Soybean Science,2010,29(02):251-254.[doi:10.11861/j.issn.1000-9841.2010.02.0251]
不同贮藏时间和处理方式对野生大豆种子萌发的影响
- Title:
- Effects of Different Storage Period and Treatment on Seed Germination of Glycine Soja
- 文章编号:
- 1000-9841(2010)02-0251-04
- Keywords:
- Glycine soja; Hard seed; Seed germination; Embryo growth
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用刀片破皮、低温冷冻、60℃热水浴及干燥、碱液处理、浓硫酸加搅拌等方法处理野生大豆种子,研究其对野生大豆种子发芽和胚生长的影响。结果表明:经过刀片破皮和浓硫酸加搅拌处理的各项萌发指标远高于对照(P<0.05);综合野生大豆幼苗的萌发指标和胚根长、胚芽及鲜重等指标,破皮和10 min 浓硫酸加搅拌处理最有利于野生大豆种子萌发;冷冻处理的各项萌发指标显著低于对照(P<0.05),与常温保存相比,冷藏保存野生大豆种子的千粒重和发芽率有所增加。表明冷藏保存可以提高野生大豆种子的质量;常温下野生大豆休眠期为7个月,冷藏保存野生大豆的休眠期为8个月。
- Abstract:
- The seed of wild soybean( Glycine soja) belong to hard seed. Its germination percentage was very low with general methods. The effect on seed germinations and embryo growth by using machinery method of damaging seed coat, freezing, 60℃ hot water bath and dryness, lye and soaked in concentrated sulfuric acid and stirred were studied in this paper. The results showed that the germination rate, viability and index with the machinery method of damaging seed coat , sulfuric acid and stirred were higher than that of the control (P<0.05). Integrate the experimental results of germination percentage, germination index and seedling growth of wild soybean, the best result was obtained when the seeds were soaked in concentrated sulfuric acid and stirred for 10 min. The germination percentage, germination viability and germination index with freezing treatment were lower than that of the control. Compared with room temperature, thousand seed weight and germination percentage increased under cold storage. The dormancy period of Glycine soja under room temperature and cold storage was 7 and 8 month, respectively.
参考文献/References:
[1]姜慧新. 浓硫酸处理对黄河三角洲野大豆发芽效果的影响[J]. 草业科学, 2005, 22(11): 58-59. (Jiang H X. The effect of concentrated sulfuric acid on germination of wild soybean in Yellow River Delta Area [J]. Pratacultural Science, 2005, 22 (11): 58-59.) [2]Dong Y S, Zhuang B C, Zhao L M, et al. The genetic diversity of annual wild soybeans grown in China[J]. Theoretieal and Applied Genetics, 2001, 103(1): 98-103. [3]府宇雷, 钱吉, 马玉虹, 等. 不同尺度下野大豆种群的遗传分化[J]. 生态学报, 2002, 22(2): 176-184. (Fu Y L, Qian J, Ma Y H, et al. Genetic differentiation research on populations of wild soybeans in different scales [J]. Acta Ecologica Sinica, 2002, 22(2): 176-184.) [4]孙学钊.黄河三角洲野生大豆硬实种子处理试验[J]. 四川草原, 1998(1): 32-36.(Sun X Z. Experiment on treating hard seeds of wild soyabean(Glycine sojaSieb. et Zucc.)in Yellow River Delta Area[J]. Journal of Sichuan Grassland, 1998(1): 32-36. [5]乔亚科, 季桂兰, 王文颇, 等. 不同处理方法和贮藏时间对野生大豆种子萌发的影响[J]. 种子,2003 (1): 33-34.(Qiao Y K, Ji G L, Qang W P, et al. Effect of different treatment methods and storage period on the germination of wild soybean[J]. Seed, 2003 (1): 33-34.) [6]李光发, 黄文, 曲刚,等. 野生大豆籽粒吸水性的探讨[J]. 大豆科学, 1994, 13 (4) : 376-379 .(Li G F,Huang W, Qu G, et al. A study on water absorbing of wild soybean seed[J]. Soybean Science, 1994, 13 (4) : 376-379.) [7]Baskin C C, Baskin J M. Seeds, ecology, biogeography, and evolution of dormancy and germination[J]. Plant Ecology, 2001,152(2): 204-205. [8]杨期和, 尹小娟, 叶万辉. 硬实种子休眠的机制和解除方法[J]. 植物学通报, 2006, 23(1): 108-118. (Yang Q H, Yin X J, Ye W H. Dormancy mechanism and breaking methods for hard seeds[J]. Chineses Bulletin of Botany, 2006, 23(1): 108-118. [9]Uzun F, Aydin I. Improving germination rate of medicago and Trifoliumapecies[J]. Asian Journal of Plant Sciences,2004, 3: 714-717. [10] 王金龙. 利用大豆硬实种子保存大豆种质的研究[J]. 大豆科学 1999, 18(4): 351-354. (Wang J L. Study on preservation of soybean germplasm using soybean hard seed. [J]. Soybean Sciense, 1999, 18(4): 351-354.) [11] Souza F D H, Marcos-Filho J. The seedcoat as a modulator of seed-environment relationships in Fabaceae[J]. Revista Brasileira de Botanica,2001, 24(4): 365-375.
相似文献/References:
[1]高越,刘辉,陶波.抗草甘膦野生大豆筛选及其抗性生理机制研究[J].大豆科学,2013,32(01):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
GAO Yue,LIU Hui,TAO Bo.Screening and Physiological Mechanisms of Resistance to Glyphosate in Wild Soybeans(Glycine soja)[J].Soybean Science,2013,32(02):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
[2]王军卫,侯立江,李? 登,等.野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
WANG Jun-wei,HOU Li-jiang,LI Deng,et al.Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(02):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
[3]王军卫,侯立江,李 登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(02):596.
[4]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(02):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[5]陈丽丽,王明玖,何丽君,等.野生大豆ISSR体系的优化及其在远缘杂交后代鉴定中的利用[J].大豆科学,2013,32(04):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
CHEN Li-li,WANG Ming-jiu,HE Li-jun,et al.Optimization for ISSR Reaction System of Wild Soybean and Its Utilization in Distant Hybrid Identification[J].Soybean Science,2013,32(02):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
[6]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(02):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[7]徐艳平,胡翠美,张文会,等.干旱胁迫对野生大豆幼苗光合作用相关指标的影响[J].大豆科学,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
XU Yan-ping,HU Cui-mei,ZHANG Wen-hui,et al.Effect of Simulated Drought Stress on Photosynthesis Related Indexes at Seedling Stage of Wild Soybeans[J].Soybean Science,2013,32(02):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
[8]胡卫静,何丽君,何劲莉,等.NaCl胁迫对野生与栽培大豆杂交后代株系生理指标的影响[J].大豆科学,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
HU Wei-jing,HE Li-jun,HE Jin-li,et al.Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max × Glycine soja[J].Soybean Science,2013,32(02):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
[9]王 旻,梁 玉,王欣欣,等.即墨野生大豆主要成分及其营养价值分析[J].大豆科学,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
WANG Min,LIANG Yu,WANG Xin-xin,et al.Assessment on Nutritional Compositions and Value of Jimo Wild Soybean[J].Soybean Science,2013,32(02):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
[10]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶(POD)活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
备注/Memo
基金项目:山东省自然科学基金资助项目(Y2008D36,Y2008D40)。