WANG Yuan-yuan,DUAN Yu-xi,CHEN Li-jie,et al.Protoplast Formation of Antagonistic Rhizobium[J].Soybean Science,2010,29(01):92-96.[doi:10.11861/j.issn.1000-9841.2010.01.0092]
拮抗性大豆根瘤菌原生质体制备研究
- Title:
- Protoplast Formation of Antagonistic Rhizobium
- 文章编号:
- 1000-9841（2010）01-0092-05
- Keywords:
- Rhizobium; Protoplast; Formation; Affecting factors
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 选用具有拮抗作用的根瘤菌L396，对影响该菌原生质体制备的几个重要因素进行了研究。结果表明：制备原生质体的最佳菌龄为对数生长中期，培养液中加入0.5%青霉素可以促进原生质体的形成，在溶菌酶浓度1.5 m·mL-1、酶解温度为35℃的情况下可以较快较好的产生原生质体，同时显微观察了原生质体的释放过程。
- Abstract:
- Several factors affecting the protoplasts formation of Sinorhizobium.Fredii with antagonistic to SCN and root rot of soybean were studied.The growth curve was determined and the optimum cell-age for protoplasts formation was in the logarithmic growth mid-phase，the maximum yield of protoplast was accelerated when pretreated by 0.5% penicillium in the culture medium，the optimum enzyme condition was digesting at 35℃ with 1.5 mg?mL-1lysozyme，the process of protoplasts release was observed using microscope.The foundation for genetic breeding of S.Fredii has been laid by using the protoplasts.
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备注/Memo
基金项目：现代大豆产业技术体系资助项目；沈阳农业大学青年教师科研基金资助项目（20070210）。