SUN Dong-mei,LIN Zhi-wei,CHI Li,et al.Preliminary Study on Induced Resistance by Trichoderma Aureoviride and Its Multiple Microbe on Soybean Sclerotinia Sclerotiorum[J].Soybean Science,2010,29(01):88-91.[doi:10.11861/j.issn.1000-9841.2010.01.0088]
黄绿木霉菌及其混剂对大豆菌核病的诱导抗性初探
- Title:
- Preliminary Study on Induced Resistance by Trichoderma Aureoviride and Its Multiple Microbe on Soybean Sclerotinia Sclerotiorum
- 文章编号:
- 1000-9841（2010）01-0088-04
- Keywords:
- Sclerotinia sclerotiorum; Trichoderm aureoviride; POD; PPO; PAL
- 分类号:
- S432.1
- 文献标志码:
- A
- 摘要:
- 采用人工接种方法，测定拮抗菌剂对大豆菌核病的抗扩展能力及相关酶活性的影响，同时研究黄绿木霉菌及其混剂对大豆菌核病的诱导抗性。结果表明：离体试验中，叶片接种后，拮抗细菌和黄绿木霉菌及其混剂均可较好的抑制菌核病病斑的进一步扩展；盆栽试验中，黄绿木霉菌处理对大豆根、第1复叶、第3复叶的PAL、PPO、POD活性均有明显增强作用，而解磷钾菌或拮抗细菌处理对PAL、PPO、POD活性的变化无明显影响，化肥处理则降低PAL、PPO、POD活性；有机肥处理对PAL活性有明显增强作用，对PPO、POD活性无明显影响。
- Abstract:
- The induced resistance of different bio-control microorganism on Sclerotinia sclerotiorum was investigated.By means of artificial inoculation method，the anti-expansion capacity of antagonistic multiple microbe on soybean Sclerotinia sclerotiorum and the associated enzyme activities were measured.The in vitro experiment showed the restrain ability of antagonism bacterium and Trichoderma aureoviride on lesion expansion by S.sclerotiorum was significant.Pot experiment showed that the PAL，PPO and POD activity of the first compound leaf，the third compound leaf and roots of soybean treated by Trichoderma aureoviride were significantly enhanced，while these enzymes of soybean treated by phosphorus and potassium were less affected，and these associated enzyme activities of soybean treated by chemical fertilizers were reduced；When treated by organic fertilizer，PAL activity was increased，while PPO and POD activitiy had no significant change.Results suggest applying of Trichoderma aureoviride and its multiple microbe is effective in controlling soybean Sclerotinia sclerotiorum.
参考文献/References:
[1]Kalogiannis S，Tjamos S E，Stergiou A，et al.Selection and evaluation of phyllosphere yeasts as biocontrol agents against grey mould of tomato[J].European Journal of Plant Pathology，2006，116(1)：69-76.
[2]翁启勇，李开本.诱导植物系统抗性研究进展[J].福建农业学报，1998.13(4)：24-28.(Weng Q Y ，Li K B.Study and progress in induction of systemic resistance of plant[J].Fujian Journal of Agricultural Sciences，1998，13(4)：24-28.)
[3]王莉.BTH诱导黄瓜和番茄对主要病害的抗性研究[D].杨凌：西北农林科技大学，2005：12-17.（Wang L.Benzothiadiazoie- Induced resistance in cucumber and tomato against primary diseases[D].Yangling：Northwest A&F University，2005：12-17.) [4]李洪连，王守正，王金生，等.黄瓜对炭疽病诱导抗性的初步研究Ⅱ.诱导抗病性机制的研究[J].植物病理学报，1993，23(4)：327-330.(Li H L，Wang S Z ，Wang J S，et al.A preliminary study on the induced resistance of cucumber against Colleotrichum lacenarium(anthracnose)Ⅱ.On the mechanism of induced resistance[J].Acta Phytopathologica Sinica，1993，23(4)：327-330.) [5]刘润进，沈崇尧，李怀方，等.VA菌根对大丽轮枝菌于棉花体内PR蛋白的诱导作用[J].植物病理学报，1993，23(2)：162-165.(Liu R J，Shen C Y，Li H F，et al.Induction of pathogenesis-related proteins in cotton seedlings by vam fungi and?Verticilium dahliae[J].Acta Phytopathologica Sinica，1993，23(2)：162-165.) [6]连玲丽.芽孢杆菌的生防菌株筛选及其抑病机理[D].福州：福建农林科技大学，2007：10-20.(Lian L L.Screening of biocontrol strains of?Bacillus?spp.and their mode of action on plant disease[D].Fuzhou：Fujian Agriculture and Forestry University，2007:10-20)
[7]Shivanna M B，Meera M S.Plant growth promoting fungi induced systemic resistance in cucumber[J].Advanced in Biological Control of Plant Disease，1996，30(3)：175-184. [8]孙冬梅，杨谦，张军政.黄绿木霉诱变菌株对大豆根腐病镰刀菌的拮抗[J].大豆科学，2005，24(3)：171-175.(Sun D M，Yang Q，Zhang J Z.Antagonism of Trichioderma aureoviride mutant strain against Fusarium spp.the pathogen of soybean root rot[J].Soybean Science，2005,24(3)：171-175.) [9]孙冬梅，杨谦，宋金柱.黄绿木霉菌代谢产物对大豆菌核病核盘菌的抑菌能力研究[J].天然产物研究与开发，2005，17(6)：691-695.(Sun D M，Yang Q，Song J Z.Study on the restrain Sclerotinia sclerotiorum by?Trichoderma aureoviride?metabolite[J].Natural Product Research and Development，2005，17(6)：691-695.)
[10]刘亚光，赵滨，马超.水杨酸和壳聚糖诱导大豆对灰斑病的抗性[J].大豆科学，2008，27(2)：296-230.(Liu Y G，Zhao B，Ma C.Induction effect of Salicylic acid and Chitosan on frogeye leaf spot in soybean[J].Soybean Science，2008，27(2)：296-230.)
[11]郑莉，梁建根，施跃峰.生防菌ZJH-10 对黄瓜灰霉病诱导抗性的研究[J].中国农学通报，2009，25(3)：197-201.（Zhen L，Liang J G，Shi Y F.Study on induced resistance of biocontrol bacteria ZJH-10 to cucumber grey mould[J].Chinese Agricultural Science Bulletin，2009，25(3)：197-201.)
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备注/Memo
基金项目：黑龙江省高校骨干教师资助项目（1152G023）。