[1]王树起,韩晓增,乔云发,等.缺磷胁迫对大豆根瘤生长和结瘤固氮的影响[J].大豆科学,2009,28(06):1000-1003.[doi:10.11861/j.issn.1000-9841.2009.06.1000]
　WANG Shu-qi,HAN Xiao-zeng,QIAO Yun-fa,et al.Nodule Growth，Nodulation and Nitrogen Fixation in Soybean(Glycine Max L.）as Affected by P Deficiency Stress[J].Soybean Science,2009,28(06):1000-1003.[doi:10.11861/j.issn.1000-9841.2009.06.1000]
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缺磷胁迫对大豆根瘤生长和结瘤固氮的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年06期 页码: 1000-1003 栏目: 出版日期: 2009-12-25
Title:
Nodule Growth，Nodulation and Nitrogen Fixation in Soybean(Glycine Max L.）as Affected by P Deficiency Stress
文章编号:
1000-9841(2009)06-1000-04
作者:
王树起1; 2; 韩晓增1; 乔云发1; 严君1; 李晓慧1
1.中国科学院 东北地理与农业生态研究所，黑龙江 哈尔滨 150081；
2.中国科学院 沈阳应用生态研究所，辽宁 沈阳 110016
Author(s):
WANG Shu-qi1; 2; HAN Xiao-zeng1; QIAO Yun-fa1; YAN Jun1; LI Xiao-hui1
1.Northeast Institute of Geography and Agricultural Ecology，CAS，Harbin 150081，Heilongjiang；
2.Institute of Applied Ecology，CAS，Shengyang 110016，Liaoning，China
关键词:
低磷胁迫; 固氮酶活性; 豆血红蛋白含量; 固氮量; 大豆
Keywords:
Phosphorus deficiency; Nitrogenase activity; Leghemoglobin; Nitrogen fixation quantity; Soybean
分类号:
S153.61
DOI:
10.11861/j.issn.1000-9841.2009.06.1000
文献标志码:
A
摘要:
采用砂培培养方法研究了缺磷胁迫对大豆根瘤生长和结瘤固氮的影响。结果表明：缺磷胁迫抑制了大豆根瘤的生长，表现为根瘤干重下降，根瘤数量减少；缺磷胁迫显著降低了大豆的固氮能力，表现为根瘤固氮酶活性和豆血红蛋白含量降低；缺磷胁迫降低了大豆的固氮量，固氮量随磷水平的增加而增加，随着生育时期的推进，固氮量表现为先增加后降低再增加的趋势，无磷(0 μmol.L-1)与高磷(50 μmol.L-1)以及低磷(30 μmol.L-1)与高磷处理间均达到了5%的显著差异。因此，缺磷胁迫抑制了大豆的结瘤固氮作用，使大豆的固氮效率降低。
Abstract:
Phosphorus deficiency stress is a serious problem in the soil，how to resolve the problem of crop production reduction resulted from P deficiency is becoming global hot research.In this paper，a sand experiment was conducted to study the effects of P deficiency stress on nodule growth，nodulation and nitrogen fixation in soybean(Glycine max L.）at glassroom condition.The results showed that P deficiency restrained nodule growth which embody in nodule dry weight declined and nodule number decreased than that of normal P supplying，meanwhile，P deficiency significantly decreased nitrogen fixation capacity of soybean which embody in nodule nitrogenase activity and leghemoglobin concentration remarkably decreased than that of normal P supplying.P deficiency reduced nitrogen fixation quantity which increased with phosphorus concentration，and the trend was rising first and then declining，finally going up with the growing stages of soybean.There is significant difference between no P（0 mol.L-1）and high P（50 mol.L-1）treatment，and low P and high P（30 mol.L-1）treatment(P<5%).Therefore，P deficiency restrained nitrogen fixation in soybean and then made the efficiency of nitrogen fixation declined.

参考文献/References:

[1]Holford C R.Soil phosphorus：its measurement，and its uptake by plants[J].Australia Journal of Soil Research，1997，35：227-239.

[2]Israel D W.Investigation of the role phosphorus in symbiotic dinitrogen fixation[J].Plant Physiology，1987，84：835-840.
[3]Jakobsen I..The role of phosphorus in nitrogen fixation by young pea plants(Pisum sativum)[J].Physiologia Plantarum，1985，64：190-196.
[4]Ohwaki Y，Sugahara P.Active extrusion of protons and exudation of carboxylic acids in response to iron deficiency by roots of chickpea（Cicer arietinum L.)[J].Plant Soil，1997，189：49-55.
[5]董钻．大豆产量生理[M]．北京：中国农业出版社.1999.(Dong Z.Yield physiology of soybean[M].Beijing：Chinese Agricultural Press,1999.)
[6]左元梅，刘永秀，张福锁.与玉米混作改善花生铁营养对其根瘤形态结构及豆血红蛋白含量的影响[J].植物生理与分子生物学学报，2003，29(1)：33-38.(Zuo Y M，Liu Y X，Zhang F S.Effects of improvement of iron nutrition by mixed cropping with maize on nodule microstructure and leghemoglobin content of peanut[J].Journal of Plant Physiology and Molecular Biology，2003，29(1)：33-38.)
[7]Felix D D.A functional relationship between leghaemoglobin and nitrogenase based on novel measurements of the two proteins in legume root nodules[J].Annals of Botany，1995，75(1)：49-54.
[8]Rachid S，Thomas R S.N2?fixation response to drought in common bean (Phaseolus vulgarisL.)[J].Annals of Botany，1998，82(2)：229-234.[9]Tang C，Hinsinger P J，Drevon J，et al.Phosphorus deficiency impairs early nodule functioning and enhances proton release in roots of Medicago truncatula L.[J].Annals of Botany，2001，88：131-138.
[10]Israel D W.Symbiotic dinitrogen fixation and host-plant growth during development of and recovery from phosphorus deficiency[J].Physiologia Plantarum，1993，88：294-300.
[11]苗淑杰，乔云发，韩晓增．两种形态氮源条件下磷对大豆结瘤固氮的影响[J]．大豆科学，2006，25(3)：250-255.(Miao S J，Qiao Y F，Han X Z.Effect of P deficiency on nodulation and function in soybean under two different N sources [J].Soybean Science，2006，25(3)：250-253，258.)
[12]Miao S J，Han X Z，Qiao Y F.Effects of seedling and nutrient solution phosphorus on nodulation and nodule function in soybean(Glycine max L.)[J].Plant Soil and Environment，2007，53(2)：65-71.
[13]Sa T M，Israel D W.Energy status and functioning of phosphorus-deficient soybean nodules[J].Plant Physiology，1991，97：928-935.

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备注/Memo

基金项目：中国科学院知识创新工程重要方向资助项目（KSCX1-YW-09-09, KSCX2-YW-N-002, KZCX2-YW-407）；国家科技支撑计划资助项目 (2006BAD21B01)。?

第一作者简介：王树起（1968-），男，副研究员，博士，现主要从事植物营养和土壤生态方面的研究。E-mail:wsq-200131@sohu.com。
通讯作者：韩晓增，研究员，博士生导师。E-mail:hanxz@cern.ac.cn。

更新日期/Last Update: 2014-09-11