[1]杨秀芳,陈梅.戊二醛交联法固定化β-D-呋喃果糖苷酶的研究[J].大豆科学,2009,28(05):902-905.[doi:10.11861/j.issn.1000-9841.2009.05.0902]
　YANG Xiu-fang,CHEN Mei.Study on Immobilized β-D-fructofuranosidase by Cross-linking with Glutaraldehyde[J].Soybean Science,2009,28(05):902-905.[doi:10.11861/j.issn.1000-9841.2009.05.0902]
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戊二醛交联法固定化β-D-呋喃果糖苷酶的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年05期 页码: 902-905 栏目: 出版日期: 2009-10-25
Title:
Study on Immobilized β-D-fructofuranosidase by Cross-linking with Glutaraldehyde
文章编号:
1000-9841(2009)05-0902-04
作者:
杨秀芳; 陈梅
陕西科技大学 教育部轻化工助剂化学与技术重点实验室，陕西 西安 710021
Author(s):
YANG Xiu-fang; CHEN Mei
Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry，Ministry of Education;Shaanxi University of Science & Technology，Xi’an 710021，Shaanxi，China
关键词:
交联法; β-D-呋喃果糖苷酶; 固定化酶
Keywords:
Cross-linking with glutaraldehyde; β-D-fructofuranosidase; Immobilization ennyme
分类号:
TS245.9
DOI:
10.11861/j.issn.1000-9841.2009.05.0902
文献标志码:
A
摘要:
大豆低聚糖属于功能性低聚糖的一种，具有促进双歧杆菌生长繁殖等生理特性。采用戊二醛交联法固定β-D-呋喃果糖苷酶。研究固定化酶法生产大豆低聚糖过程中对酶固定化制备的条件、操作稳定性。采用戊二醛交联法固定β-D-呋喃果糖苷酶。结果表明：最佳固定化条件为：戊二醛浓度20 mmol.L-1，蛋白质浓度1.0%，酶与固定液之比1∶10，固定化时间2 h；此条件下制备的固定化酶平均酶活达340 U.g-1，酶活保留率80%以上。固定化酶在间歇反应器中具有较高的操作稳定性，固定化酶的半衰期为58 d，完全可以满足工业化生产的要求。
Abstract:
The soybean oligosaccharide is a kind of functional oligosaccharides,which can multiply the number of Bifidobacteria.A novel method to immobilize β-D-fructofuranosidase by cross-linking with glutaraldehyde was successfully constructed.The preparation condition and operation stability of immobilization enzyme were investigated during the process of soybean oligosaccharide production.A novel method to immobilize β-D-fructofuranosidase by cross-linking with glutaraldehyde was successfully constructed.The conditions of immobilization were investigated by orthogonal experiment method.The optimal conditions of immobilization investigated by orthogonal experiment method were as follows：the concentration of glutaraldehyde and protein were 20 mmol.L-1and 1.0%，the ratio of enzyme and immobilization liquld was 1∶10，the immobilizing time was 2 h.Under the optimal condition the average activity of immobilized enzyme reached 340 U.g -1，after immobilization and the activity could retain more than 80%.The immobilized enzyme showed high operation stability in batch reactor，whose half-life was 58 d.The stability of immobilized enzyme could completely meet the need of industrial production.

参考文献/References:

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备注/Memo

基金项目：陕西省教育厅自然科学基金资助项目（ 08JK238）。

作者简介：杨秀芳（1963-），女，教授，研究方向为天然产品的研究与开发。E-mail：yangxf@sust.edu.cn。

更新日期/Last Update: 2014-09-18