[1]刘彩霞,梁成珠,徐彪,等.抗草甘膦转基因大豆及加工品LAMP检测研究[J].大豆科学,2009,28(02):305-309.[doi:10.11861/j.issn.1000-9841.2009.02.0305]
　LIU Cai-xia,LIANG Cheng-zhu,XU Biao,et al.Detection of Genetically Modified Soybean and Products by LAMP Reaction[J].Soybean Science,2009,28(02):305-309.[doi:10.11861/j.issn.1000-9841.2009.02.0305]
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抗草甘膦转基因大豆及加工品LAMP检测研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 305-309 栏目: 出版日期: 2009-04-25
Title:
Detection of Genetically Modified Soybean and Products by LAMP Reaction
文章编号:
1000-9841(2009)02-0305-05
作者:
刘彩霞; 梁成珠; 徐彪; 高宏伟; 林超; 孙敏
山东出入境检验检疫局，检验检疫技术中心，山东 青岛 266002
Author(s):
LIU Cai-xia; LIANG Cheng-zhu; XU Biao; GAO Hong-wei; LIN Chao; SUN Min
Technical Center of Inspection and Quarantine，Shandong Administration for Entry-Exit Inspection and Quarantine,Qingdao 266002，Shandong China
关键词:
环介导等温核酸扩增; 快速检测; 抗草甘膦转基因; EPSPS基因
Keywords:
Loop mediated isothermal amplification; Rapid detection; Roundup Ready;  EPSPS gene
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0305
文献标志码:
A
摘要:
将环介导的等温核酸扩增技术应用于转基因大豆及加工品检测。针对抗草甘膦Roundup Ready转基因大豆及加工品外源基因EPSPS设计2对特异性引物进行扩增，成功建立起定性检测转基因大豆及加工品的LAMP检测方法。优化LAMP反应条件，反应温度为65℃，反应时间为1h。结果表明：该体系能快速、灵敏、有效地检测转基因大豆及加工品中整合的EPSPS基因，检测限为0.01%，低于国际现行最低检测量0.5%的要求。检测EPSPS基因操作简单，成本低、特异性强、灵敏度高。LAMP检测结果可信，稳定性好，可对目前批准的抗草甘膦Roundup Ready转基因大豆及加工品进行定性检测。
Abstract:
The loop-mediated isothermal amplification(LAMP)that amplifies DNA with high specificity and rapidity under an isothermal condition was applied for rapid detection of Roundup Ready and its products.A set of four primers，two outer and two inner primers，was designed specifically to recognize EPSPS gene of Roundup Ready.The LAMP reaction mix was optimized.The optimal reaction temperature and time of the LAMP assay for EPSPS gene were 65℃ and 1 h，respectively.The LAMP detection method has been secessfully set up.The results showed that the LAMP reaction system can detect EPSPS gene effectively and sensitively.The limit of detection is under 0.01% lower than the international limit.These results suggest that detection of EPSPS by LAMP is an effective and low-cost procedure with high specificity and sensitivty that requires no specialized equipment.This asssy is expected to become a valuable tool for rapid detection and identification of Roundup Ready soybean and its products.

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备注/Memo

基金项目：国家质量监督检验检疫总局科研资助项目（20071K167）。

作者简介：刘彩霞（1981-），女，硕士，工程师。主要从事出入境转基因食品检测研究。E-mail:emlcai@163.com。

更新日期/Last Update: 2014-10-04