[1]郑树贵,曹松屹,孙泽威,等.β-伴大豆球蛋白β亚基的纯化及免疫活性鉴定[J].大豆科学,2009,28(02):301-304,309.[doi:10.11861/j.issn.1000-9841.2009.02.0301]
　ZHENG Shu-gui,CAO Song-yi,SUN Ze-wei,et al.Purification of β Subunit from β-conglycinin and Identification of Immunological Activity of the Prepared Subunit[J].Soybean Science,2009,28(02):301-304,309.[doi:10.11861/j.issn.1000-9841.2009.02.0301]
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β-伴大豆球蛋白β亚基的纯化及免疫活性鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 301-304,309 栏目: 出版日期: 2009-04-25
Title:
Purification of β Subunit from β-conglycinin and Identification of Immunological Activity of the Prepared Subunit
文章编号:
1000-9841(2009)02-0301-04
作者:
郑树贵1; 2; 曹松屹3; 孙泽威1; 秦贵信1
1吉林农业大学动物科技学院，吉林 长春130118；
2沈阳农业大学畜牧兽医学院，辽宁 沈阳 110161；
3沈阳农业大学生物技术学院，辽宁 沈阳 110161
Author(s):
ZHENG Shu-gui1; 2; CAO Song-yi3; SUN Ze-wei1; QIN Gui-xin1
1College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，Jilin;
2College of Animal Husbandry and Veterinary Medicine，Shenyang Agricultural University，Shenyang 110161，Liaoning;
3College of Biological Technology，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
β-伴大豆球蛋白; β亚基; 纯化; 免疫活性
Keywords:
β-conglycinin; β subunit; Purification; Immunological activity
分类号:
TQ936.21+2
DOI:
10.11861/j.issn.1000-9841.2009.02.0301
文献标志码:
A
摘要:
等电点沉淀法和凝胶过滤层析提纯的β-伴大豆球蛋白经6 molL-1脲变性后解聚为游离亚基，利用DEAE琼脂糖凝胶F F离子交换层析分离纯化β-伴大豆球蛋白亚基，并对纯化亚基进行透析复性。同时将β-伴大豆球蛋白免疫家兔制备抗血清，利用免疫印迹方法检测纯化的亚基与β-伴大豆球蛋白抗血清是否发生免疫反应。结果表明:利用DEAE琼脂糖凝胶F F阴离子交换层析可以纯化出高纯度的β-伴大豆球蛋白β亚基，纯化的β亚基可以与抗β-伴大豆球蛋白抗体结合，具有免疫活性。
Abstract:
Purified β-conglycinin prepared by electric-point precipitation and filtration chromatography was denatured by 6 molL-1urea and separated into free subunits.The subunits of β-conglycinin were isolated and purified by DEAE-Sepharose F F anion exchange chromatography and the prepared subunits were renatured by dialysis.The rabbits were immunized by β-conglycinin to produce β-conglycinin special antiserum.The immune responses between purified subunits and the antiserum were determined by immunoblotting.The results indicated：pure β subunit could be prepared from β-conglycinin by DEAE-Sepharose F F anion exchange chromatography，and the purified β subunit could specially bind to β-conglycinin antiserum and this proved that the subunit had immunological activity.

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备注/Memo

基金项目：国家自然科学基金重点资助项目(30430520)。

作者简介：郑树贵(1972-)，男，讲师，博士研究生，现从事大豆抗营养因子研究。E-mail:zhengshugui1001@163.com。

更新日期/Last Update: 2014-10-04