[1]苗淑杰,乔云发,韩晓增.磷和根瘤菌交互作用对大豆结瘤和生长的影响[J].大豆科学,2009,28(02):271-274.[doi:10.11861/j.issn.1000-9841.2009.02.0271]
　MIAO Shu-jie,QIAO Yun-fa,HAN Xiao-zeng.Effect of Phosphorus and Rhizobium on Growth and Nodule Development in Soybean[J].Soybean Science,2009,28(02):271-274.[doi:10.11861/j.issn.1000-9841.2009.02.0271]
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磷和根瘤菌交互作用对大豆结瘤和生长的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 271-274 栏目: 出版日期: 2009-04-25
Title:
Effect of Phosphorus and Rhizobium on Growth and Nodule Development in Soybean
文章编号:
1000-9841(2009)02-0271-04
作者:
苗淑杰; 乔云发; 韩晓增
中国科学院东北地理与农业生态研究所，黑龙江 哈尔滨 150081
Author(s):
MIAO Shu-jie; QIAO Yun-fa; HAN Xiao-zeng
Northeast Institute of Geography and Agricultural Ecology，CAS，Harbin 150081，Heilongjiang，China
关键词:
磷; 根瘤菌; 大豆; 根瘤生长
Keywords:
Phosphorus; Rhizobium; Soybean; Nodule development
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0271
文献标志码:
A
摘要:
为了阐明营养液中磷浓度和不同数量根瘤菌的交互作用对大豆结瘤和生长的影响，在营养液培养条件下，进行3种磷水平[P1-0、P2-4和P3-30(μmol P.L-1)]和3种根瘤菌浓度[R1-102、R2-103.5和R3-105（CFUmL-1）]处理，培养大豆25 d，测定大豆生物量和根瘤性状指标。结果表明：在相同根瘤菌数量条件下，不同磷浓度对大豆总生物量的影响不大，对根冠比的影响较大。营养液中接种的根瘤菌浓度越高，根瘤原基数量越大，低磷明显抑制根瘤原基发育形成根瘤，进而减少了低磷处理大豆根瘤数量。从P1到P3，R1根瘤菌浓度处理根瘤原基形成根瘤的几率分别为：79%、86%和100%。营养液中磷浓度促进生物量向根瘤中分配，高磷处理相对根瘤生物量较高。随着生育进程，大豆各组织器官含磷量逐渐降低，在所有取样时期含磷量均表现为根瘤>根>地上部；含氮量表现为根瘤>地上部>根。可见磷和根瘤菌对大豆生长和结瘤形成有交互作用。
Abstract:
To study the interaction of phosphorus(P)level and rhizobium content on nodulation and nodule development in soybean，soybean was grown in nutrient solution culture for 25 days.We designed three solution P levels as P1-0，P2-4 and P3-30(μmol PL-1)，and three rhizobium contents were R1-102，R2-103.5?and R3-105（CFUmL-1）.Soybean biomass and nodulation characters were measured after treatment.Results showed that P had little effect on total plant biomass，however，affected the ratio of root to shoot in this experiment.Nodule initiation increased as rhizobium content increase，but P deficiency inhibited nodule initiation，so nodule number was lower in low phosphorus treatment.From P1 to P3 level，the ratio of nodule initiation to nodule was 79%，86% and 100%.Relocate of biomass to nodule was affected by P concentration in nutrient solution.P concentration in tissues decreased as growing，which was in an order of nodule>root>shoot；N concentration was in an order of nodule>shoot>root.All these suggested that the interaction of phosphorus level and rhizobium content had important effect on soybean growth and nodule formation.

参考文献/References:

[1]Marschner H.Mineral nutrition of higher plants[M].2nd?ed.London：Academic Press，1995：899.

[2]Robson A D，O`Hara G W，Abbott L K.Involvement of phosphorus in nitrogen fixation by sybterranean clover(Trifolium subterraneum L.)[J].Australian Journal of Plant Physiology，1981，8：427-436.
[3]Jakobsen I.The role of phosphorus in nitrogen fixation by young pea plants(Pisum sativum)[J].Physiologia Plantarum，1985，64:190-196.
[4]Israel D W.Investigation of the role phosphorus in symbiotic dinitrogen fixation[J].Plant Physiology，1987，84：835-840.
[5]Israel D W.Symbiotic dinitrogen fixation and host-plant growth during development of and recovery from phosphorus deficiency[J].Physiologia Plantarum，1993，88:294-300.
[6]Drevon J J，Hartwig U A.Phosphorus deficiency increased the argon-induced decline of nodule nitrogenase activity in soybean and alfalfa[J].Planta，1997，201：463-469.
[7]Almeida J P F，Hartwig U A，Frehner M，et al.Evidence that P deficiency induces feedback regulation of symbiotic N2?fixation in white clover(Trifolium repens L.)[J].Journal of Experimental Botany，2000，51：1289-1297.
[8]Hellsten A，Huss Danell K.Interaction effects of nitrogen and phosphorus on nodulation in red clover(Trifolium pratense L.)[J].Acta Agriculturae Scandinavica，Section B，Soil and Plant Science，2001，50:135-142.
[9]Yang Y.The effects of phosphorus on nodule formation in the Casuarina-Frankia symbiosis[J].Plant and Soil，1995，176:161-169.
[10]Reddell P，Yang Y，Shipton W A.Do Casuarina cunninghamiana seedlings dependent on symbiotic N2?fixation have higher phosphorus requirement than those supplied with adequate fertilizer nitrogen[J].Plant and Soil，1997，189：213-219.
[11]Sanginga N，Danso S K A，Bowen G D.Nodulation and growth response of Allocasuarina and Casuarina species to phosphorus fertilization[J].Plant Soil，1989，118:125-132.
[12]Tang C，Hinsinger P J，Drevon J J，et al.Phosphorus deficiency impairs early nodule functioning and enhances proton release in roots of Medicago truncatula L[J].Annals of Botany，2001，88：131-138.
[13]左元梅，刘永秀，张福锁.N 3 -态氮对花生结瘤与固氮作用的影响[J].生态学报.2003，23(4)：758-764.(Zuo Y M，Liu Y X，Zhang F S.Effects of the NO-3N on nodule formation and nitrogen fixing of peanut[J].Acta Ecologica Sinica，2003，23(4)：758-764.)
[14]Voisin A S，Salon C，Jeudy C，et al.Root and nodule growth in Pisum sativum L.in relation to photosynthesis：analysis using -13C-labelling[J].Annals of Botany，2003，92：557-563.

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备注/Memo

基金项目：国家重点基础研究发展计划（973计划）资助项目(2005CB121101)；黑龙江省攻关资助项目（GB05C201-01）；中国科学院东北地理与农业生态研究所青年博士基金资助项目。

作者简介：苗淑杰（1975-）女，博士，主要从事植物营养生理方面研究。E-mail:miaoshujie@126.com。

更新日期/Last Update: 2014-10-03