[1]牛丽颖,田鹏娜,李清,等.豆豉对早期动脉粥样硬化大鼠主动脉平滑肌细胞凋亡的影响[J].大豆科学,2009,28(01):156-159.[doi:10.11861/j.issn.1000-9841.2009.01.0156]
　NIU Li-ying,TIAN Peng-na,LI Qing,et al.Effects of Semen Sojae Preparatum on the Apoptosis of Aortic Smooth Muscle Cells in Rats With Early Atherosclerosis[J].Soybean Science,2009,28(01):156-159.[doi:10.11861/j.issn.1000-9841.2009.01.0156]
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豆豉对早期动脉粥样硬化大鼠主动脉平滑肌细胞凋亡的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年01期 页码: 156-159 栏目: 出版日期: 2009-02-25
Title:
Effects of Semen Sojae Preparatum on the Apoptosis of Aortic Smooth Muscle Cells in Rats With Early Atherosclerosis
文章编号:
1000-9841(2009)01-0156-04
作者:
牛丽颖1; 田鹏娜2; 李清1; 刘娇1; 王鑫国1
1河北医科大学中医学院，河北 石家庄 050091；
2保定市第三医院药剂科，河北 保定 071000
Author(s):
NIU Li-ying; TIAN Peng-na; LI Qing; LIU Jiao; WANG Xin-guo
1College of Traditional Chinese Medicine，Hebei Medical University，Shijiazhuang 050091，Hebei;
2 Pharmacy Department of the Third Hospital in Baoding City,Baoding 071000,Hebei,China
关键词:
豆豉; 动脉粥样硬化; 细胞凋亡; 增殖指数; 突变型p53; Fas
Keywords:
Soybean ; Atherosclerosis ; Apoptosis; Proliferation indexes; p53; Fas
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0156
文献标志码:
A
摘要:
观察豆豉对早期动脉粥样硬化大鼠主动脉平滑肌细胞凋亡及相关凋亡基因突变型p53和Fas蛋白表达的调节作用，以探讨其机制。采用高脂饲料喂养法建立大鼠早期动脉粥样硬化模型，同时连续10周灌胃给予豆豉提取物进行干预。末次给药后处死动物，光镜下观察血管平滑肌细胞的形态，透射电镜观察超微结构，采用流式细胞术检测细胞凋亡率以及凋亡相关基因突变型p53和Fas蛋白的表达。模型对照组主动脉平滑肌细胞的凋亡率及增值指数(FI)明显高于正常对照组（P< 0.05），p53蛋白表达上调；豆豉干预组（20 g生药kg-1、40 g生药kg-1）大鼠主动脉平滑肌细胞的凋亡率明显高于模型对照组（P< 0.05），增殖指数明显低于模型对照组(P<0.05)，Fas蛋白表达上调(P< 0.05)，p53蛋白表达下调（P< 0.05），光镜及透射电镜可见主动脉损伤明显较模型对照组改善。豆豉可调节早期动脉粥样硬化大鼠血管平滑肌细胞凋亡与增殖的平衡，其机制可能与调节突变型p53和Fas蛋白的表达有关。
Abstract:
To investigate the effects of Semen Sojae Preparatum（SSP)on apoptosis，and gene expression of p53 and Fas in aortic smooth muscle cells（ASMCs)in early Atherosclerosis(AS）rats，and to explore the possible mechanism.Rat models with early AS were induced by feeding fatty foods，meanwhile rats in two treated groups were orally administered with extract of SSP for 10 weeks.All rats were killed and samples of ASMCs harvested.The cellular morphology was observed by light microscope and ultrastructure by transmission electron microscopy；apoptosis and gene expression of p53 and Fas were detected by flow cytometry.Compared with normal group，the apoptosis rate and the proliferation index(PI）in model group were obviously increased（P<0.01），with higher gene expression of p53；moreover compared with model group，the apoptosis rates in two treated groups（20 gkg-1，40 gkg-1）were higher（P<0.01)，the proliferation index was lower（P<0.01)，and the gene expression of Fas was increased，but p53 was decreased（P<0.05）；histologically，the aortic damage was remarkably improved with SSP.These results suggest that SSP can adjust the balance between apoptosis and proliferation of ASMC，which may relate to gene expression regulation of p53 and Fas.

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备注/Memo

基金项目：牛丽颖（1968-），女，教授，硕士生导师，现主要从事中药物质基础研究工作。

作者简介：王鑫国，教授，硕士生导师，E-mail：wangxinguozy@163.com。

更新日期/Last Update: 2014-10-03