[1]陈金峰,胡斌杰,韩艳霞,等.钙对铜胁迫下大豆种子的解毒作用[J].大豆科学,2008,27(06):1072-1075.[doi:10.11861/j.issn.1000-9841.2008.06.1072]
　CHEN Jin-feng,HU Bin-jie,HAN Yan-xia,et al.Detoxification of Calcium on Soybean Seeds Under Copper Stress[J].Soybean Science,2008,27(06):1072-1075.[doi:10.11861/j.issn.1000-9841.2008.06.1072]
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钙对铜胁迫下大豆种子的解毒作用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年06期 页码: 1072-1075 栏目: 出版日期: 2008-12-25
Title:
Detoxification of Calcium on Soybean Seeds Under Copper Stress
文章编号:
1000-9841(2008)06-1072-04
作者:
陈金峰; 胡斌杰; 韩艳霞; 杨晓柯
开封大学化学工程学院，河南 开封 475004
Author(s):
CHEN Jin-feng; HU Bin-jie; HAN Yan-xia; YANG Xiao-ke
College of Chemical Engineering Kaifeng University，Kaifeng 475004，Henan，China
关键词:
铜胁迫; 钙; 大豆; 保护酶
Keywords:
Copper stress; Calcium; soybean; Protective enzymes
分类号:
Q171.5
DOI:
10.11861/j.issn.1000-9841.2008.06.1072
文献标志码:
A
摘要:
钙对铜胁迫下大豆种子解毒作用的研究，对获得优良抗逆性状和提高大豆产量具有重要的意义。设置0、0.4、0.8、2.4和4 mmol.L-1铜离子浓度梯度，同时在各浓度梯度中分别添加4.5和9.0 mmol.L-1的钙离子，进行了种子萌发试验，测定了相对电导率和保护酶活性。结果显示：铜对大豆种子的发芽活性具有抑制作用，且随胁迫浓度的增加这种抑制作用加强，钙离子对0.8 mmol.L-1铜离子的解毒作用效果明显，能够提高种子的活力指数和根长，减少电解质渗透率。4.5 mmol.L-1钙能够增加铜胁迫下大豆种子中POD活性，而降低CAT活性，两种酶存在着一种协调关系。说明钙对一定浓度的铜胁迫能够起到缓解作用，其中保护酶参与了细胞膜完整性的维持。
Abstract:
To obtain soybean superior character with stress resistance and higher yield，the study of calcium on soybean seeds under copper stress is important.The seeds was soaked in different copper concentrations of 0，0.4，0.8，4 mmol.L-1，supplied with 4.5，9.0 mmol.L-1 calcium，and then the seeds germination，relative permeability and activities of protective enzymes were investigated.The results showed the seed vigor was decreased with the increasing of copper concentration.Addition of calcium could alleviate the toxicity of 0.8 mmol.L-1 copper to soybean seeds，especially increasing seed activity index，improving root length，and reducing the relative permeability of cell membrane.Under different copper concentrations，4.5 mol.L-1calcium could increase pod activity and decrease CAT activity.The results indicated that calcium alleviated copper toxicity on soybean seeds to a certain extent，and protective enzymes could maintain the integrity of cell membrane.

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备注/Memo

基金项目：开封大学2007年高等职业教育教改立项资助项目（2007-KDJG-11)。

作者简介：陈金峰（1977-），男，硕士，讲师，现从事生物化学与分子生物学的教学及研究。E-mail:cjfchengsman@163.com。

更新日期/Last Update: 2014-10-06