[1]李为喜,刘方,李燕,等.反相高效液相色谱法测定大豆籽粒中异黄酮含量的方法研究[J].大豆科学,2008,27(05):828-832,837.[doi:10.11861/j.issn.1000-9841.2008.05.0828]
　LI Wei-xi,LIU Fang,LI Yan,et al.Determination of Isoflavone Content in Soybean by Reverse Phase High Performance Liquid Chromatography[J].Soybean Science,2008,27(05):828-832,837.[doi:10.11861/j.issn.1000-9841.2008.05.0828]
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反相高效液相色谱法测定大豆籽粒中异黄酮含量的方法研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年05期 页码: 828-832,837 栏目: 出版日期: 2008-10-25
Title:
Determination of Isoflavone Content in Soybean by Reverse Phase High Performance Liquid Chromatography
文章编号:
1000-9841(2008)05-0828-05
作者:
李为喜; 刘方; 李燕; 刘三才; 朱志华
中国农业科学院作物科学研究所，国家农作物基因资源与基因改良重大科学工程，农业部作物品种资源监督检验测试中心，北京100081
Author(s):
LI Wei-xi; LIU Fang; LI Yan; LIU San-cai; ZHU Zhi-hua
The National Key Facilities for Crop Genetic Resources and Improvement，Institute of Crop Science，CAAS，The Supervision and Testing Center for Crop Germplasm Resources，MOA，Beijing 100081，China
关键词:
反相高效液相色谱法; 黄豆苷; 染料木苷; 黄豆黄素苷; 行业标准
Keywords:
RP-HPLC; Daidzin; Genistin; Glycitin; Agricultural standard
分类号:
S565.1Q501
DOI:
10.11861/j.issn.1000-9841.2008.05.0828
文献标志码:
A
摘要:
大豆异黄酮是一类具有特殊生理活性物质。研究并建立了应用反相高效液相色谱法测定大豆籽粒中黄豆苷、黄豆黄素苷和染料木苷3种异黄酮成分含量的检测方法。同时，运用统计学原理对建立的大豆异黄酮测定方法进行了系统、全面的分析和评价。结果表明：黄豆苷、黄豆黄素苷和染料木苷线性范围分别为1.25~20.0 μg.mL-1、0.625~10.0 μg.mL-1和1.25~20.0 μg.mL-1；回收率范围为97.1%~99.5%；重复性测定的相对相差均低于5%；不同实验室对黄豆苷、黄豆黄素苷和染料木苷测定的相对标准偏差分别为1.15%~3.42%、2.44%~4.84%和1.43%~3.06%。上述结果说明该方法的线性、准确度、精密度、同一实验室重复性和多家实验室的再现性评价结果优良，适合作为国家农业行业标准的制定和实施。
Abstract:
Isoflavone is a group of special biofunctional substance.In this paper，a method determining isoflavone including Daidzin，Glycitin and Genistin by Reverse Phase High Performance Liquid Chromatography (RP-HPLC) was investigated and erected.Meanwhile，the isoflavone determination method was analysed as well as evaluated wholly and systematically by statistical technique.The results showed that the linear range of Daidzin，Glycitin and Genistin was 1.25-20.0μg.mL-1，0.625-10.0μg.mL-1 and 1.25-20.0μg.mL-1，respectively；the recovery range was 97.1%-99.5%；the relative deviation of repeatable test was below 5%；the relative standard deviation of Daidzin，Glycitin and Genistin among different labs was 1.15%-3.42%，2.44%-4.84% and 1.43%-3.06%，respectively.It showed from the above analysis that this method possessed good linearity，good quality of accuracy，precision，repeatability in one lab and reproducibility among labs.Therefore，this method was proved to be suitable for erecting and implementing a national agricultural standard.

参考文献/References:

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备注/Memo

基金项目：农业部保种项目（NB05-070401-26 -8）；农业行业标准制（修）订项目（05253）；国家植物种质资源共享平台建设资助项目（2005DKA21001-07）；中央级科研单位基本科研基金资助项目。

作者简介：李为喜（1975-），男，硕士，助理研究员，主要从事作物种质资源营养成分及检测技术研究。E-mail: aoccgt@sina.com。

更新日期/Last Update: 2014-10-06