[1]蒋向辉,佘朝文,李丹,等.野生大豆丛生芽诱导及快速繁殖[J].大豆科学,2008,27(04):697-700.[doi:10.11861/j.issn.1000-9841.2008.04.0697]
　JIANG Xiang-hui,SHE Chao-wen,LI Dan,et al.Caespitose Shoots Induction and Rapid Propagation of G.soja[J].Soybean Science,2008,27(04):697-700.[doi:10.11861/j.issn.1000-9841.2008.04.0697]
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野生大豆丛生芽诱导及快速繁殖

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年04期 页码: 697-700 栏目: 出版日期: 2008-08-25
Title:
Caespitose Shoots Induction and Rapid Propagation of G.soja
文章编号:
1000-9841(2008)04-0697-04
作者:
蒋向辉1; 2; 佘朝文1; 2; 李丹1; 郝博飞1
1怀化学院生物工程系，
2怀化市生物育种与加工技术实验室，湖南 怀化418008
Author(s):
JIANG Xiang-hui1; 2; SHE Chao-wen1; 2; LI Dan1; HAO Bo-fei1
1Department of Bioengineering；
2Key Laboratory of Huaihua for Breeding and Bioprocess Technology，Huaihua College，Huaihua 418008，Hunan，China
关键词:
野生大豆; 丛生芽; 快速繁殖
Keywords:
G.soja; Caespitose shoots; Rapid propagation
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0697
文献标志码:
A
摘要:
野生大豆(G.soja)是豆科大豆属Soja亚属的唯一野生种，为了有效地保护我国的野生大豆资源，推动优异的野生大豆材料在育种及生物技术中的应用，以野生大豆无菌苗幼叶为外殖体，采用正交试验设计方法，对影响野生大豆丛生芽发生和生根诱导的因素进行比较研究。结果表明：适合野生大豆丛生芽诱导的培养基为MS + 6-BA 1.5 mg.L-1+ IBA 2.0 mg.L-1+ NAA 0.3 mg.L-1，适合生根的培养基是1/2 MS + IBA 1.0 mg.L-1+ IAA 1.2 mg.L-1，在此条件下获得大量移栽成活的植株。
Abstract:
G.soja is the only wild germplasm of Soja subgenus,in order to protect G.soja resource availably and expedite the application of excellent resource in breeding and biotechnology,young leaf of G.soja was used as explants for rapid propagation,the effect of different culture medium and concentration of phytohormon on inducing caespitose shoots and roots of G.soja was studied using an orthogonal experimental designed.The results showed that the best medium inducing caespitose shoots of G.soja was MS + 6-BA 1.5 mg.L-1+ IBA 2.0 mg.L-1+ NAA 0.3 mg.L-1,the proper rooting medium is 1/2 MS+IBA 1.0 mg.L-1+ IAA 1.2 mg.L-1，and many regeneration plantlets were obtained.

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备注/Memo

基金项目：怀化市科技局科技基础条件平台建设资助项目（06-6）。

作者简介：蒋向辉（1974-）男，硕士，讲师，现从事植物遗传育种研究。

更新日期/Last Update: 2014-10-07