[1]王志坤,李文滨,刘珊珊.RNA干扰技术及在植物抗病研究中的应用[J].大豆科学,2008,27(03):521-526.[doi:10.11861/j.issn.1000-9841.2008.03.0521]
　WANG Zhi-kun,LI Wen-bin,LIU Shan-shan.Technology of RNAi and Its Application in Disease Resistance of Plant[J].Soybean Science,2008,27(03):521-526.[doi:10.11861/j.issn.1000-9841.2008.03.0521]
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RNA干扰技术及在植物抗病研究中的应用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 521-526 栏目: 出版日期: 2008-06-25
Title:
Technology of RNAi and Its Application in Disease Resistance of Plant
文章编号:
1000-9841(2008)03-0521-06
作者:
王志坤; 李文滨; 刘珊珊
东北农业大学大豆所，大豆生物学教育部重点实验室，黑龙江 哈尔滨 150030
Author(s):
WANG Zhi-kun; LI Wen-bin; LIU Shan-shan
Soybean Research Institute of Northeast Agricultural University，Chinese Education Ministry’s Key Laboratory of Soybean Biology，Harbin 150030，Heilongjiang，China
关键词:
RNAi; 作用机制; 基因沉默; 抗线虫; 抗病毒病
Keywords:
RNA interference; Molecular mechanism; Gene silencing; Control of plant parasitic nematodes; Antivirus
分类号:
S565.101
DOI:
10.11861/j.issn.1000-9841.2008.03.0521
文献标志码:
A
摘要:
RNA干扰（RNA interference，RNAi）技术是一项基因沉默新技术，自20世纪90年代被发现以来，现在已逐渐成为分子生物学和细胞生物学研究的有用工具之一。目前该技术已被广泛应用到植物功能基因组研究和植物抗病毒病研究中，在抗线虫病研究中应用较少。现综述了RNAi的分子机制、基因沉默的主要类型以及该技术在植物抗线虫病和抗病毒病研究中的应用。
Abstract:
The technology of RNA interference(RNAi)is a new technology of gene silencing.Since RNAi was found in 1990s，it has been one of the most useful tool for molecular and cellular biology.RNAi has been applied in plant functional genomics and antivirus，but few applications in control plant parasitic nematodes were elucidated.In this review，we elucidated RNAi molecular mechanism，types of gene silencing and its application in control of plant parasitic nematodes and plant virus.

参考文献/References:

[1]Napoli C，Lemieux C，Jorgensen R.Introduction of a chimeric chalcone synthase gene into Petunia results in reversible cosuppression of homologous gene in trans [J].Plant Cell，1990，2:279-289.

[2]Guo S，Kemphues K J.Par-1，a gene required for establishing polarity in C.elegans embryos，encodes a putative Ser/Thr kinase that is asymmet rically distributed [J].Cell，1995，81(4): 611-620.
[3]Fire A，Xu S Q，Montgomery M K，et al.Potent and specific interference by double-stranded RNA in Caenorhabditis elegans[J].Nature，1998.391: 806-811.?
[4]王贤，滑冰.RNA干扰作用及其技术[J].周口师范学院学报，2003,20（5）：47-50.（Wang Xian，Hua Bing.RNA interfernce and technology[J].Journal of Zhoukou Teachers College，2003，20(5):47-50.）
[5]Lipardi C，Wei Q，Paterson B M.RNAi as random degradative PCR: siRNA primers convert mRNA into dsRNAS that are degraded to generate new siRNAs[J].Cell，2001，107(3):297-307.
[6]Stoutjesdijk P A，Singh S P，Liu Q，et al.hpRNA-mediated targeting of the Arabidopsis FAD2 gene gives highly efficient and stable silencing[J].Plant Physiology Preview，2002，129:1-9.
[7]Wesley S V，Helliwell C A，Smith N A，et al.Construct design for efficient，effective and high-throughput gene silencing in plants[J].Plant Journal，2001，27(6):581-590.
[8]Chitwood D J.Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture-Agricultural Research Service [J].Pest Management Science，2003，59:748-753.
[9]Atkinson H J，Urwin P E，McPherson M J.Engineering plants for nematode resistance[J].Annual Reviews in Phytopathology，2003,41:615-639.
[10]Urwin P E，Lilley C J，Atkinson H J.Ingestion of double-stranded RNA by preparasitic juvenile cyst nematodes leads to RNA interference[J].Molecular Plant-Microbe Interactions，2002，15: 747-752.
[11]Bakhetia M，Charlton W，Atkinson H J，et al.RNA interference of dual oxidase in the plant nematode Meloidogyne incognita[J].Molecular Plant-Microbe Interactions，2005，18:1099-1106.
[12]Chen Q，Rehman S，Smant G，et al.Functional analysis of pathogenicity proteins of the potato cyst nematode Globodera rostochiensis-using RNAi[J].Molecular Plant-Microbe Interactions，2005，8:621-625.
[13]Fanelli E，Di Vito M，Jones J T，et al.Analysis of chitin synthase function in a plant parasitic nematode，Meloidogyne artiellia，using RNAi[J].Gene，2005，349:87-95.
[14]Lilley C J，Goodchild S A，Atkinson H J，et al.Cloning and characterization of a Heterodera glycines aminopeptidase cDNA[J].International Journal for Parasitology，2005，35:1577-1585.
[15]Rosso M N，Dubrana M P，Cimbolini N，et al.Application of RNA interference to root-knot nematode genes encoding esophageal gland proteins[J].Molecular Plant-Microbe Interactions，2005，18:615-620.
[16]GheysenG,VanholmeB.RNAi from plants to nematodes[J].Trends in Biotechnology，2007，25: 89-92.
[17]Huang G Z，Allen R，Davis E L，et al.Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene[J].PNAS，2006，103:14302-14306.
[18]Yadav B C，Veluthambi K，Subramaniam K.Host-generated double stranded RNA induces RNAi in plant-parasitic nematodes and protects the host from infection [J].Molecular & Biochemical Parasitology，2006，148: 219-222.
[19]Steeves R M，Todd T C，Essig J S，et al.Transgenic soybeans expressing siRNAs specific to a major sperm protein gene suppress-Heterodera glycines-reproduction [J].Functional Plant Biology，2006，33: 991-999.
[20]Roberts T M,Steward M.Nematode sperm locomotion[J].Current Opinion in Cell Biology，1995，7:13-17.
[21]Bottino D，Mogilner A，Roberts T，et al.How nematode sperm crawl[J].Journal of Cell Science，2002，115:367-384.
[22]Fairbairn D J，Cavallaro A S，Bernard M，et al.Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes[J].Planta，2007，226(6): 1525-1533.
[23]Jacobs JJ，Sanders M，Bots M，et al.Sequences throughout the basic β-1，3-glucanase mRNA coding region are targets for homology-dependent post-transcriptional silencing[J].Plant J，1999，20:143-152.
[24]Kerschen A，Napoli C A，Jorqensen R A，et al.Effectiveness of RNA interference in transgenic plants[J].FEBS Lett，2004，566: 223-228.
[25]Hussey R S and Mims CW.Ultrastructure of feeding tubes formed in giant cells induced in plants by the root-knot nematode Meloidogyne incognita[J].Protoplasma，1991，162: 99-107.
[26]Bockenhoff A,Crundler F MW.Studies on nutrient uptake by the beet cyst nematode-Heterodera schachtii-by in situ microinjection of fluorescent probes into the feeding structures in Arabidopsis thaliana[J].Parasitology，1994，109: 249-254.
[27]Urwin P E，Lilley C J，McPherson M J，et al.Resistance to both cyst and root-knot nematodes conferred by transgenic Arabidopsis-expressing a modified plant cystatin[J].Plant Journal，1997,12: 455-461.
[28]Bakhetia M，Charlton W L，Urwin P E，et al.RNA interference and plant parasitic nematodes[J].Trends Plant Science，2005，10: 362-367.
[29]Pinto Y M，Kok R A,Bandcombe D C.Resistance to rice yellow mottle virus(RYMV)in cultivated African rice varieties containing RYMV transgenes[J].Nat.Biotechnol.，1999，17(7):702-707.
[30]Wang M B，Abbott D C,Waterhouse P M.A single copy of a virus-derived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus[J].Mol.Plant Pathol，2000，1(6):347-356.
[31]Tenllado F,Diaz-Ruiz J R.Double-stranded RNA-mediated interference with plant virus infection[J].J Virol，2001，75(24):12288-12297.
[32]Tenllado F，Llave C,Diaz-Ruiz J R.RNA interference as a new biotechnological tool for the control of virus diseases in plants [J].Virus Research，2004，102: 85-96.
[33]Kalantidis K，Psaradakis S，Tabler，M，et al.The occurrence of CMV-specific short RNAs in transgenic tobacco expressing virus-derived double-stranded RNA is indicative of resistance to the virus [J].MPMI，2002，15(8): 826-833.
[34]Takahashi S，Komatsu K，Kagiwada S，et al.The efficiency of interference of Potato virus X infection depends on the target gene [J].Virus Research，2006，116: 214-217.

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备注/Memo

基金项目：黑龙江省博士后资助项目（LBH-Z07247）。

作者简介：王志坤（1978-），女，助理研究员，博士，主要从事大豆遗传改良与分子生物学基础研究。E-mail:zhikunwang1998@yahoo.com.cn。

更新日期/Last Update: 2014-10-08