[1]陈婷婷,曹光群,杨成.聚乙二醇包覆的维生素C脂质体的制备及性质研究[J].大豆科学,2008,27(03):505-508.[doi:10.11861/j.issn.1000-9841.2008.03.0505]
　CHEN Ting-ting,CAO Guang-qun,YANG Chen.Preparation and Property of Peg-coated Vitamin C-containing Liposome[J].Soybean Science,2008,27(03):505-508.[doi:10.11861/j.issn.1000-9841.2008.03.0505]
点击复制

聚乙二醇包覆的维生素C脂质体的制备及性质研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 505-508 栏目: 出版日期: 2008-06-25
Title:
Preparation and Property of Peg-coated Vitamin C-containing Liposome
文章编号:
1000-9841(2008)03-0505-04
作者:
陈婷婷; 曹光群; 杨成
江南大学化学与材料工程学院，江苏 无锡 214122
Author(s):
CHEN Ting-ting; CAO Guang-qun; YANG Chen
School of Chemical and Material Engineering，Jiangnan University，Wuxi 214122，Jiangsu，China
关键词:
维生素Ｃ; 脂质体; 聚乙二醇
Keywords:
Vitamin C; Liposome; Polyethylene glycol(PEG)
分类号:
TQ658
DOI:
10.11861/j.issn.1000-9841.2008.03.0505
文献标志码:
A
摘要:
利用聚乙二醇（PEG）包覆脂质体以提高脂质体的稳定性。先用薄膜分散－超声乳化法制备维生素C脂质体，然后将PEG溶液与维生素C脂质体混合制备PEG包覆的脂质体。电镜照片证明PEG在脂质体外形成了一层膜，粒径增加。脂质体经PEG包覆后包封率增加。稳定性试验测定不同温度下脂质体吸光度变化及脂质体中丙二醛含量变化情况，得到较佳贮存条件为4 ℃避光，PEG包覆后的脂质体吸光度与丙二醛含量增大幅度均较小。结果表明：用PEG可有效包覆维生素C脂质体并提高脂质体的稳定性。
Abstract:
The purpose of this work was to improve the stability of liposome by coating PEG.The vitamin C-containing liposome，which had been prepared by film dispersion-ultrasonic emulsify method，was coated with PEG by simply mixing the liposomal suspension with the PEG solution.The structure determined by electron microscope confirmed the existence of polymer layer on the surface of liposome particles.By comparison with the non-coated liposome，the polymer coated one had larger particle size and better encapsulation.The changes of A500nmand the malondialdehyde(MDA) content in liposome in different temperature were investigated，the optimal storage condition was light-avoiding at 4 ℃，and the polymer coated one showed less increase in A500nmand MDA.Results suggest that the vitamin C-containing liposome could be effectively coated by PEG and its stability was improved.

参考文献/References:

[1] Kristl J，Volk B，Gaǎperlin M，et al.Effect of colloidal carriers on ascorbyl palmitate stability[J].European Journal of Pharmaceutical Sciences，2003，19：181-189.

[2]Woodle M C，Lasic D D.Sterically stabilized liposome[J].Biochimica et Biophysica Acta，1992，1113：171-199.
[3]Zanin M H A，Torriani I C L，Zollner R L，et al.Physical characterization of surface-modified liposomes by incorporation of gangliosides designed for immunotherapies[J].Colloids and Surfaces A： Physicochem.Eng.Aspects，2004，251：175-182.
[4]Tirosh O，Barenholz Y，Katzhendler J，et al.Effect of grafted PEG on liposome size and on compressibility and packing of lipid bilayer[J].Chemistry and Physics of Lipids，2005，135： 117-129.
[5]刘亚文，曹光群，陈婷婷.维生素C脂质体的制备研究[J].大豆科学，2007，26(2)：270-272.(Liu Y W，Cao G Q，Chen T T.Study on preparation of vitamin C liposome[J].Soybean Science，2007，26(2)：270-272.)
[6]Takeuchi H，Yamamoto H，Toyoda T，et al.Physical stability of size controlled small unimaller liposome coated with a modified polyvinyl alcohol[J].International Journal of Pharmaceutics，1998，164：103-111.
[7]王岩，周莉玲.青藤碱脂质体的制备及稳定性研究[J].中成药，2005，27(2)：757-760.(Wang Y，Zhou L L.Study on preparation and stability of sinomenine liposomes[J].Chinese Traditional Patent Medicine，2005，27(2)：757-760.)
[8]张灵芝.脂质体制备及其在生物医学中的应用[M].北京：北京医科大学，中国协和医科大学联合出版社，1998：57-58.(Zhang L Z.Preparation of liposome and its application in biomedicine[M].Beijing：Peking University Health Science Center，Peking Union Medical College Press，1998：57-58.)
[9]朱斌，许时婴，夏书芹.薄膜水化法制备辅酶Q10脂质体[J].食品与机械，2006，22(6)：39-41.(Zhu B，Xu S Y，Xia S Q.Preparation of coenzyme Q10liposomes by thin-film rehydration method[J].Food & Machinery，2006，22(6)：39-41.)
[10]孙京新，周光宏，徐幸莲.猪肉微粒体脂肪酸氧化产物对氧合肌红蛋白氧化的影响[J].南京农业大学学报，2004，27(1)：101-104.(Sun J X，Zhou G H，Xu X L.Effect of oxidation products of fatty acids in microsomes from pork on oxymyoglobin oxidation in vitro[J].Journal of Nanjing Agricultural University，2004，27(1)：101-104.)
[11]陈涛，王昭，傅经国，等.聚乙二醇修饰脂质体研究[J].世界最新医学信息文摘，2003，2(5)：801-805.(Chen T，Wang Z，Fu J G，et al.Research of modifying liposome with polyethylene glycol[J].Digest of The World Latest Medical Information，2003，2(5)：801-805.)

相似文献/References:

[1]雷俊,郭娜,孙聚涛,等.高维生素C含量大豆芽芽用品种筛选[J].大豆科学,2012,31(05):771.[doi:10.3969/j.issn.1000-9841.2012.05.017]
　LEI Jun,GUO Na,SUN Jü-tao,et al.Screening of Soybean Germoplasms for Growing Sprout Based on Vitamin C Content[J].Soybean Science,2012,31(03):771.[doi:10.3969/j.issn.1000-9841.2012.05.017]
[2]穆筱梅,钟振声,杨梅.脂质体用大豆卵磷脂的制备及质量评价[J].大豆科学,2007,26(02):250.[doi:10.3969/j.issn.1000-9841.2007.02.027]
　MU Xiao-mei,ZHONG Zhen-sheng,YANG Mei.PREPARATION AND QUALITY EVALUATION OF SOYBEAN PHOSPHATIDYLCHOLINE AS LIPOSOMAL MATERIAL[J].Soybean Science,2007,26(03):250.[doi:10.3969/j.issn.1000-9841.2007.02.027]
[3]穆筱梅,钟振声.大豆磷脂脂质体的制备[J].大豆科学,2007,26(03):386.[doi:10.3969/j.issn.1000-9841.2007.03.021]
　MU Xiao-mei,ZHONG Zhen-sheng.PREPARATION OF LIPOSOME MADE OF SOYBEAN LECITHIN[J].Soybean Science,2007,26(03):386.[doi:10.3969/j.issn.1000-9841.2007.03.021]
[4]穆筱梅 　 钟振声.维生素 E 、维生素 C 和 BHT 对大豆磷脂脂质体的抗氧化作用*[J].大豆科学,2006,25(04):435.[doi:10.11861/j.issn.1000-9841.2006.04.0435]
　Mu Xiaomei Zhong Zhensheng.ANTIOXIDATION EFFECTS OF VITAMIN E,VITAMIN C AND BHTON THESOYBEAN PHOSPHOLIPID LIPOSOME[J].Soybean Science,2006,25(03):435.[doi:10.11861/j.issn.1000-9841.2006.04.0435]
[5]刘亚文,曹光群,陈婷婷.维生素C脂质体的制备研究[J].大豆科学,2007,26(02):270.[doi:10.3969/j.issn.1000-9841.2007.02.031]
　LIU Ya-wen,CAO Guang-qun,CHEN Ting-ting.STUDY ON PREPARATION OF VITAMIN C LIPOSOME[J].Soybean Science,2007,26(03):270.[doi:10.3969/j.issn.1000-9841.2007.02.031]

备注/Memo

基金项目：国家自然科学基金资助项目（20506008）。

作者简介：陈婷婷（1984-），女，硕士研究生，研究方向为精细化学品开发。
通讯作者：曹光群，高级工程师。E-mail: gqcao@jiangnan.edu.cn。

更新日期/Last Update: 2014-10-08