[1]李秀侠,吴海燕,时立波,等.大豆胞囊线虫白色胞囊与褐色胞囊几种代谢物质比较[J].大豆科学,2008,27(02):283-287.[doi:10.11861/j.issn.1000-9841.2008.02.0283]
　LI Xiu-xia,WU Hai-yan,SHI Li-bo,et al.Comparative Studies on Metabolites in White and Brown Cyst of Heterodera Glycines Race 4[J].Soybean Science,2008,27(02):283-287.[doi:10.11861/j.issn.1000-9841.2008.02.0283]
点击复制

大豆胞囊线虫白色胞囊与褐色胞囊几种代谢物质比较

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 283-287 栏目: 出版日期: 2008-04-30
Title:
Comparative Studies on Metabolites in White and Brown Cyst of Heterodera Glycines Race 4
文章编号:
1000-9841(2008)02-0283-05
作者:
李秀侠1; 吴海燕1; 时立波1; 王振华1; 于浩2
1 山东农业大学植物保护学院，山东 泰安 271018；
2 黑龙江省农垦科学院，黑龙江 佳木斯 154007
Author(s):
LI Xiu-xia1; WU Hai-yan1; SHI Li-bo1; WANG Zhen-hua1; YU Hao2
1 College of Plant Protection，Shandong Agricultural University，Tai’an 271018，Shandong；
2Academy of Land-reclaimable Sciences of Heilongjiang Province，Jiamusi 154007，Heilongjiang，China
关键词:
大豆胞囊线虫; 糖类; 酯酶; 海藻糖酶; 蛋白质
Keywords:
Soybean cyst nematode; Carbohydrate; Esterase; Trehalase; Protein
分类号:
S435.651
DOI:
10.11861/j.issn.1000-9841.2008.02.0283
文献标志码:
A
摘要:
大豆胞囊线虫成熟后以胞囊的形式存在于土壤中，并由白色逐渐变成褐色。对白色胞囊和褐色胞囊的孵化率以及几种代谢物质（总糖，糖原，海藻糖，甘油，蛋白质，酯酶，海藻糖酶）进行了比较。结果表明：褐色胞囊的孵化率明显比白色胞囊的低；褐色胞囊内的糖原、蛋白质含量较低，酯酶活性较小，但是总糖，海藻糖含量较高，海藻糖酶活性较高，说明褐色胞囊内卵的滞育率较高，其代谢物质中海藻糖和糖原之间可能存在活跃的相互转化；SDS-PAGE蛋白图谱中白色胞囊和褐色胞囊的谱带分别为15和10条，白色胞囊比褐色胞囊多116.6，79.1，68.2，64.3和60.5 KDa等5条谱带；酯酶电泳图谱中白色胞囊和褐色胞囊的谱带分别为3和6条，褐色胞囊比白色胞囊多EST-0.63、EST-0.75、EST-0.81等3条谱带。说明新形成的白色胞囊中蛋白质含量较高，在胞囊褐化的过程中，蛋白质含量下降，酯酶同工酶的活性增强。褐色胞囊内卵的孵化率低可能与这些物质变化相关。
Abstract:
Previous research stated that the hatch rate of white cysts was significantly different from that of brown cysts，which indicated that there was different diapause rate between white and brown cysts.In order to find out the relationships between some metabolites and diapause of Heterodera glycines，hatch rate and metabolites（total carbohydrate，glycogen，trehalose，glycerol，protein，esterase，trehalase) in white and brown cysts of Heterodera glycines race 4 were studied.The results suggested that hatch rate of brown cyst was lower significantly than that of white cyst.Content of glycogen and protein and activity of esterase in brown cys were lower than that of white cyst，content of total carbohydrate and trehalose and activity of trehalase in brown cyst was higher.Therefore，diapause rate of brown cysts was higher than that of white cysts，and active mutual transformation between trehalose and glycogen existed in brown cysts.SDSPAGE pattern showed that there are 15 and 10 protein bands in white and brown cysts respectively，and molecular weight 116.6，79.1，68.2，64.3 and 60.5 KDa proteins existed in white cysts，and without in brown cysts.There were 3 and 6 bands in patterns of esterase isoenzyme in white and brown cysts respectively，EST-0.63，EST-0.75 and EST-0.81 of esterase isoenzyme occurred in brown cysts，and not in white cysts.The result suggested that content of protein was declined，and activity of esterase isoenzyme was enhanced during the white cyst change to the brown cyst.And deduced that low hatch rate of eggs in brown cysts might be related to those metabolites.

参考文献/References:

[1] Slack D A，Hamblen M L.The effect of various factors on larval emergence from cysts of Heterodera glycines[J].Journal of Nematology，1960,7：350-355.

[2]Wu H Y，Duan Y X，Li X X.Dormancy of the soybean cyst nematode Heterodera glycines[J].Acta Zoological Sinica，2006,52(3)：498-503.
[3]Hominick W M，Forrest J M S，Evans A A F.Diapause in Globodera rostochiensis and variability in hatching trials[J].Nematologica，1985,31：159-170.
[4]Ash C P J，Atkinson H J.Nematodirus battus：Development of cold hardiness in dormant eggs[J].Experimental Parasitology，1986,62：24-28.
[5]Storey R M J.The relationship between neutral lipid reserves and infectivity for hatched and dormant juveniles of Glo bodera spp[J].Annals of Applied Biology，1984,104：511-520.
[6]Nicholas W L.The biology of free-living nematodes.Second Edition[M].Oxford:Oxford University Press，1984:119-138.
[7]刘维志.植物病原线虫学[M].北京：中国农业出版社，2000.(Liu W Z.Plant nematology[M].Beijing:China Agricultural Press，2000.)
[8]冯慧.昆虫生物化学分析法[M].北京：农业出版社，1989:8-10.(Feng H.Biochemistry analysis of insect[M].Beijing:Agricultural Press，1989:8-10.)
[9]Wu J X，Yuan F.Changes of glycerol content in diapause larvae of the orange wheat blossom midge，sitodiplosis mosellana（genin) in various seasons[J].Entomologia Sinica，2004,11(1)：27-35.
[10]萧能赓，余瑞元，袁明秀.生物化学实验原理和方法[M].北京：北京大学出版社，2006.(Xiao N G，Yu R Y，Yuan M X.Principle and methods of biochemistry experiments[M].Beijing:Beijing University Press，2006.)
[11]陈巨莲，倪汉祥，孙京瑞，等.小麦几种主要次生物质对麦长管蚜几种酶活力的影响[J].昆虫学报，2003,46(2)：144-149.(Chen J L，Ni H X，Sun J R，et al.Effects of major secondary chemicals of wheat plants on enzyme activity in Sitobion Avenae[J].Acta Entomologica Sinica，2003,46(2)：144-149.)
[12]雷芳，张桂芬，万方浩，等.寄主转换对B型烟粉虱和温室粉虱的海藻糖含量和海藻糖酶活性的影响[J].中国农业科学，2006,39(7)：1387-1394.(Lei F，Zhang G F，Wan F H，et al.Effects of plant species switching on contents and dynamics of trehalose and thehalase activity of Bemisia tabaci B-biotype and trialeurodes vaporariorum[J].Scientia Agricultura Sinica，2006,39(7)：1387-1394.)
[13]Wharton D A，Judge K F，Worland M R.Cold acclimation and cryoprotectants in a freeze tolerant Antarctic nematode，Panagrolaimus davidi[J].Journal of Comparative Physiology B，2000,170：321-327.
[14]Wharton D A，Young S R，Barrett J.Cold tolerance in nematodes[J].Journal of Comparative Physiology B，1984,154：73-77.
[15]陈田飞，乐波灵.家蚕滞育机理研究概况[J].广西蚕业，2004,41(3)：12-16.(Chen T F，Le B L.Survey of mechanism studies on diapause in the silkworm[J].Guangxi Sericulture，2004,41(3)：12-16.)
[16]Sommerville R L,Davey K G.Diapause in parasitic nematodes：a review[J].Canadian Journal of Zoology，2002,80(11)：1817-1840.
[17]王荫长.昆虫生物化学[M].北京：中国农业出版社，2001.(Wang Y Z.Biochemistry of insect[M].Beijing:China Agricultural Press，2001.)
[18]李毅平，龚和，朴镐用.松针瘿蚊越冬幼虫体内酶活性的时序变化[J].昆虫学报，2000,43(3)：227-232.(Li Y P，Gong H，Pu G Y.Profile of enzymic activity in overwintering mature larvae of the pine needle gall midge，Thecodiplosis japonensis[J].Acta Entomologica Sinica，2000,43(3)：227-232.)

相似文献/References:

[1]李 凯,刘志涛,李海朝,等.国家大豆区域试验品种对SMV和SCN的抗性分析[J].大豆科学,2013,32(05):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
　LI Kai,LIU Zhi-tao,LI Hai-chao,et al.Resistance to Soybean Mosaic Virus and Soybean Cyst Nematode of Soybean Cultivars from China National Soybean Uniform Trials[J].Soybean Science,2013,32(02):670.[doi:10.11861/j.issn.1000-9841.2013.05.0670]
[2]郑雅楠,陈乐,陈井生,等.休眠期大豆胞囊线虫体内关键酶活性变化[J].大豆科学,2013,32(04):526.[doi:10.11861/j.issn.1000-9841.2013.04.0526]
　ZHENG Ya-nan,CHEN Le,CHEN Jing-sheng,et al.Changes of Related Enzyme Activities in Dormant Soybean Cyst Nematode,Heterodera glycines[J].Soybean Science,2013,32(02):526.[doi:10.11861/j.issn.1000-9841.2013.04.0526]
[3]李泽宇,李肖白,陈井生,等.大豆品种（系）抗大豆胞囊线虫14号生理小种的抗性鉴定研究[J].大豆科学,2014,33(03):408.[doi:10.11861/j.issn.1000-9841.2014.03.0408]
　LI Ze-yu,LI Xiao-bai,CHEN Jing-sheng,et al.Identification of Soybean Varieties for Resistance to Soybean Cyst Nematode Races 14[J].Soybean Science,2014,33(02):408.[doi:10.11861/j.issn.1000-9841.2014.03.0408]
[4]袁翠平,赵洪锟,王玉民,等.利用SSR标记评价抗胞囊线虫野生大豆种质的遗传多样性[J].大豆科学,2014,33(02):147.[doi:10.11861/j.issn.1000-9841.2014.02.0147]
　YUAN Cui-ping,ZHAO Hong-kun,WANG Yu-min,et al.Genetic Diversity of Wild Soybean(Glycine soja)Resistant Germplasms to Soybean Cyst Nematode Revealed by SSR Markers[J].Soybean Science,2014,33(02):147.[doi:10.11861/j.issn.1000-9841.2014.02.0147]
[5]李晓明,杜春梅,郑楠,等.芽孢杆菌BL-21、HNDF2对大豆胞囊线虫抑制效果的研究[J].大豆科学,2011,30(04):710.[doi:10.11861/j.issn.1000-9841.2011.04.0710]
　LI Xiao-ming,DU Chun-mei,ZHENG Nan,et al.Inhibitory Effect of Bacillus BL-21,HNDF2 on Soybean Cyst Nematode[J].Soybean Science,2011,30(02):710.[doi:10.11861/j.issn.1000-9841.2011.04.0710]
[6]郑雅楠,王媛媛,陈井生,等.大豆胞囊线虫热激蛋白基因Hsp70的克隆与原核表达[J].大豆科学,2012,31(02):198.[doi:10.3969/j.issn.1000-9841.2012.02.008]
　ZHENG Ya-nan,WANG Yuan-yuan,CHEN Jing-sheng,et al.Cloning and Prokaryotic Expression of Hsp70 from Soybean Cyst Nematode(Heterodera glycines)[J].Soybean Science,2012,31(02):198.[doi:10.3969/j.issn.1000-9841.2012.02.008]
[7]刘丹丹,段玉玺,陈立杰.酸类化合物对大豆胞囊线虫防效及寄主生长的影响[J].大豆科学,2012,31(02):278.[doi:10.3969/j.issn.1000-9841.2012.02.024]
　LIU Dan-dan,DUAN Yu-xi,CHEN Li-jie.Effect of Acid Compound on Soybean Cyst Nematode and Host Growth[J].Soybean Science,2012,31(02):278.[doi:10.3969/j.issn.1000-9841.2012.02.024]
[8]胡 新,许艳丽,LI Shu-xian,等.利用抗感品种混种防治大豆胞囊线虫效果的研究[J].大豆科学,2012,31(03):449.[doi:10.3969/j.issn.1000-9841.2012.03.023]
　HU Xin,XU Yan-li,LI Shu-xian,et al.Effect of Cultivar Mixture on Growth and Developoment of Soybean Inoculated with Soybean Cyst Nematode[J].Soybean Science,2012,31(02):449.[doi:10.3969/j.issn.1000-9841.2012.03.023]
[9]宋洁,许艳丽,姚钦,等.尿素对大豆胞囊线虫的抑制作用[J].大豆科学,2012,31(05):784.[doi:10.3969/j.issn.1000-9841.2012.05.020]
　SONG Jie,XU Yan-li,YAO Qin,et al.Suppression of Urea on Soybean Cyst Nematode[J].Soybean Science,2012,31(02):784.[doi:10.3969/j.issn.1000-9841.2012.05.020]
[10]胡海波,韩英鹏,滕卫丽,等.重组自交系群体对大豆胞囊线虫3号生理小种抗性与主要农艺性状的相关分析[J].大豆科学,2012,31(05):793.[doi:10.3969/j.issn.1000-9841.2012.05.022]
　HU Hai-bo,HAN Ying-peng,TENG Wei-li,et al.Correlation between Resistance to Soybean Cyst Nematode Race No.3 and Major Agronomic Traits of Recombinant Inbred Lines[J].Soybean Science,2012,31(02):793.[doi:10.3969/j.issn.1000-9841.2012.05.022]

备注/Memo

基金项目：中国博士后科学基金资助项目（20070411104）。

作者简介：李秀侠（1981-），女，硕士研究生，现主要从事植物线虫学研究。

更新日期/Last Update: 2014-10-10