[1]周艳利,李建科,温艳霞.用于农药残留检测的大豆酯酶的纯化分离[J].大豆科学,2007,26(06):935-938，942.[doi:10.3969/j.issn.1000-9841.2007.06.025]
　ZHOU Yan-li,LI Jian-ke,WEN Yan-xia.PURIFICATION AND SEPARATION OF SOYBEAN ESTERASE FOR PESTICIDE RESIDUE DETECTION[J].Soybean Science,2007,26(06):935-938，942.[doi:10.3969/j.issn.1000-9841.2007.06.025]
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用于农药残留检测的大豆酯酶的纯化分离

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年06期 页码: 935-938，942 栏目: 出版日期: 2007-12-25
Title:
PURIFICATION AND SEPARATION OF SOYBEAN ESTERASE FOR PESTICIDE RESIDUE DETECTION
文章编号:
1000-9841(2007)06-0935-04
作者:
周艳利; 李建科; 温艳霞
陕西师范大学食品工程与营养科学学院，西安 710062
Author(s):
ZHOU Yan-li; LI Jian-ke; WEN Yan-xia
College of Food Engineering and Nutritional Science，Shaanxi Normal University,Xi’an 710062
关键词:
农药残留; 快速检测; 大豆酯酶; 纯化; 分离
Keywords:
Pesticide residues; Rapid detection; Soybean Esterase; Purification; Separation
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.06.025
文献标志码:
A
摘要:
以大豆种子为原料探索农药残留检测用酯酶的新酶原。采用差素离心、硫酸铵盐析、DEAE-32离子交换层析和Sephadex G-100凝胶过滤层析等方法对大豆酯酶进行了纯化分离，并研究了纯化酶对有机磷和氨基甲酸酯类农药的敏感性。最终得到3种电泳纯的大豆酯酶，记为E1,E2和E3。但3种大豆酯酶对有机磷和氨基甲酸酯类农药的敏感性不同，E1对农药没有敏感性，E2的敏感性比E3好。因此，大豆酯酶E2可作为农药残留检测的新酶原。
Abstract:
To search a new esterase for pesticide residue detection from soybean seeds，the soybean seed powder was dealt with crude extraction，differential centrifugation，ammonium sulfate salting-out to purify soybean esterase.The purified enzyme was then separated by DEAE-32 ion exchange column chromatography and Sephadex G-100 gel filtration column chromatography.Then，the inhibition to Organophosphate and Carbamate pesticides of the esterase was observed.Three soybean esterase of electrophoresis purity were obtained，marked with E1，E2and E3.Rusults indicated that E1had no sensitivity (inhibition) to Organophosphate and Carbamate pesticide while Ewas better sensitive than E3.Hence，a new esterase for pesticide residue detection from soybean seeds and a better purification method were obtained.

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备注/Memo

基金项目：国家科技攻关项目（2001BA804A28）；陕西省科技攻关项目（2004K01-G7）；西安市科技攻关项目（GG05112）

作者简介：周艳利（1983-），女，硕士，研究方向为食品营养与卫生。E-mail:zhouyanli83@stu.snnu.edu.cn
通讯作者：李建科，教授。E-mail：jiankel@snnu.edu.cn

更新日期/Last Update: 2014-10-19