[1]穆筱梅,钟振声.大豆磷脂脂质体的制备[J].大豆科学,2007,26(03):386-390.[doi:10.3969/j.issn.1000-9841.2007.03.021]
　MU Xiao-mei,ZHONG Zhen-sheng.PREPARATION OF LIPOSOME MADE OF SOYBEAN LECITHIN[J].Soybean Science,2007,26(03):386-390.[doi:10.3969/j.issn.1000-9841.2007.03.021]
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大豆磷脂脂质体的制备

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年03期 页码: 386-390 栏目: 出版日期: 2007-06-25
Title:
PREPARATION OF LIPOSOME MADE OF SOYBEAN LECITHIN
文章编号:
1000-9841(2007)03-0386-05
作者:
穆筱梅1; 钟振声2
1.仲恺农业技术学院化学与化工系，广州 510225；
2.华南理工大学化学学院， 广州 510640
Author(s):
MU Xiao-mei1; ZHONG Zhen-sheng2
1.Department of Chemistry & Chemical Engineering,Zhongkai University of Agriculture and Technology,Guangzhou 510225;
2.College of Chemistry,South China Univ of Tech,Guangzhou,510641
关键词:
脂质体; 大豆磷脂;  包封率; 泄露率
Keywords:
Liposome; Soybean lecithin; Entrapping efficienc; Leakage rate
分类号:
TQ645.9+6
DOI:
10.3969/j.issn.1000-9841.2007.03.021
文献标志码:
A
摘要:
以3种不同的大豆磷脂为材料，用反相蒸发法制备脂质体,通过对其形态、粒径、包封率、泄漏率和稳定性的测定，研究大豆磷脂组成对脂质体性质的影响。结果表明，脂质体为圆球状的小囊泡，以含有磷脂酰胆碱（PC）93.62％的磷脂SPC-3为材料制备的脂质体相比较于以PC含量分别为31.29％和58.19％的磷脂SPC-1和SPC-2制备的脂质体，具有最大的包封率、粒径和最小的泄漏率。稳定性研究结果表明以磷脂SPC-1为材料制备的脂质体稳定性好。
Abstract:
The liposomes made with three kinds of soybean lecithin composed of phosphatidylcholine( PC)，phosphatidyletthanolamine (PE)，phosphatidylinositol ( PI) and phosphatidic( PA) was prepared by using reverse phrase evaporation method.The effect of the soybean lecithin composition on the physiochemical properties by investigating diameter,zeta potential，entrapping efficiency,leakage rate and stability .The Transmission electron micrographs showed that all the liposomes were spherical-shape.The liposome made of Lecithin SPC-3 which contains 93.62％PC showed large diameter, higher entrapping efficiency and lower leakage rate than that of lecithin SPC-1 and Lecithin SPC-2 which contain 31.29％and 58.19％ PC separately.The liposome made of lecithin SPC-1 was more stable than that of lecithin SPC-2 and lecithin SPC-3 due to the smallest zeta potential of Lecithin SPC-1

参考文献/References:

［1］张灵芝.脂质体制备及其在生物医学中的应用［M］.北京：北京医科大学、北京协和医科大学联合出版社，1998：1-53.

［2］Soka FC,Papahadjopoulos D.Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse evaporation [J].Process of National Science .1978,75(9):4194-4198
［3］Takeuchi Hirofumi,Yamamoto Hiromitsu,Toyoda Toshitad,et al.Physical stability of size controlled small unilameller liposomes coated with a modified polyvinyl alcohol［J］.International Journal of Pharmaceutics，1998,164(1-2):103-111.
［4］刘辉,汤韧,何晓霞，等.脂质体处方和制备方法对阿昔洛韦棕搁酸酷脂质体稳定性的影响[J］.药学学报，2002;37(7):563-566

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备注/Memo

作者简介：穆筱梅（1973-），女，讲师，主要从事大豆磷脂的精制以及脂质体的研究。

更新日期/Last Update: 2014-10-22