[1]刘昭军,王德国,李铁,等.野生大豆根际微生物的分离及其缓解大豆连作障碍的研究[J].大豆科学,2007,26(02):176-180.[doi:10.3969/j.issn.1000-9841.2007.02.012]
　LIU Zhao-jun,WANG De-guo,LI Tie,et al.ISOLATION OF BACTERIA IN WILD SOYBEAN RHIZOSPHERE AND STUDY ON ITS FUNCTION TO REDUCE DISADVANTAGE OF SOYBEAN CONTINUOUS CROPPING[J].Soybean Science,2007,26(02):176-180.[doi:10.3969/j.issn.1000-9841.2007.02.012]
点击复制

野生大豆根际微生物的分离及其缓解大豆连作障碍的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年02期 页码: 176-180 栏目: 出版日期: 2007-04-25
Title:
ISOLATION OF BACTERIA IN WILD SOYBEAN RHIZOSPHERE AND STUDY ON ITS FUNCTION TO REDUCE DISADVANTAGE OF SOYBEAN CONTINUOUS CROPPING
文章编号:
1000-9841(2007)02-0176-05
作者:
刘昭军; 王德国; 李铁; 刘丽艳; 雷勃钧; 李柱刚
1.黑龙江省农业科学院生物技术研究中心，黑龙江省作物与家畜分子育种重点实验室,哈尔滨 150086
Author(s):
LIU Zhao-jun; WANG De-guo;  LI Tie;  LIU Li-yan;  LEI Bo-jun;  LI Zhu-gang
1.Biotechnology Research Center of Heilongjiang Academy of Agricultural Sciences;
2.Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang ,Harbin 150086
关键词:
野生大豆; 微生物; 大豆连作
Keywords:
Glycine soja; Microbe; Soybean continuous cropping
分类号:
S565.1;S154.3
DOI:
10.3969/j.issn.1000-9841.2007.02.012
文献标志码:
A
摘要:
利用唯一碳源培养基筛选野生大豆根际微生物，分离获得5株分别降解邻苯二甲酸、丙二酸、乙酸、丙酸、苯等大豆化感物质的微生物，分别命名为HK14-2、HK26-2、HN31-1、HK46-2、HK53-1。通过单菌落培养、显微镜观察、革兰氏染色以及PCR-DGGE电泳试验证明，筛选的5种微生物均为细菌， HK26-2为杆菌，其余为球菌，且均为纯系。菌落平皿对峙试验证明5种细菌均可拮抗大豆立枯丝核菌；不同菌剂的大豆盆栽试验证明5种细菌均可降低大豆根腐病发生率，其中HN31-1与HK53-1复配菌剂的防效高达69.5%。统计根系的主根长以及根干重的数据表明：筛选菌剂的添加增加了大豆的主根长以及根干重，改善大豆根际微生态环境。初步认为所分离野生大豆根际细菌可以缓解大豆连作障碍。
Abstract:
Five bacterial strains were isolated from wild soybean rhizosphere soil in medium contained only one kind of carbonaceous organic compound. They could digest phthalate, malonic acid, acetic acid, propionic acid and benzene and were named HK14-2, HK26-2, HN31-1, HK46-2 and HK53-1, respectively. Through single colony culture, microscope observe, Gram’s dying and PCR-DGGE electrophoresis, it was showed that all of the screened five microbe were bacteria, HK26-2 was bacillus and others were coccus, and the isolated bacteria strain was pure. Test of colony standoff in dish proved the five bacteria could resist soybean pathogenic fungi(Rhizoctonia.solani). Potted soybean treated with different bacteria showed that the five bacteria could decrease the occurrence of soybean root rot, and the highest control effectiveness was 69.5% with compound bacteria of HN31-1 and HK53-1. Compared with the control, statistical data of taproot length and root dry weight showed that use of screened bacteria increased the numerical value and improved the environment of soybean rhizosphere. It was considered that the isolated bacteria from wild soybean rhizosphere could have biological control efficacy against pathogens of soybean root rot.

参考文献/References:

［1］刘佩印.黑龙江省大豆重迎茬问题的研究概况[J].黑龙江农业科学2001,(3):60－64.

[2]徐凤花,汤树德,孙冬梅,等.重迎茬对大豆根际微生物的影响[J].黑龙江八一农垦大学学报,1998,10(1):5－8.
[3]何志鸿,刘忠堂,许艳丽,等.大豆重迎茬减产的原因及农艺对策研究―重迎茬大豆的根际土壤有机化合物[J].黑龙江农业科学, 2003,(5):1－5.
[4]谌晓曦,陈卫良,李德葆.细菌及其拮抗物质对植物病原真菌的抑制[J].微生物学通报. 2003,30(3):67－71.
[5]韩丽梅,沈其荣,王树起,等.大豆根茬木霉腐解产物的鉴定及其化感作用的研究[J].应用生态学报,2002,(10):97－101.
[6]胡江春,薛德林,王书锦,等.大豆连作障碍研究Ⅲ、海洋放线菌MB－97促进大豆连作增产机理[J].应用生态学报,2002:1095－1098.
[7]王克晶,李福山.我国野生大豆 (G.soja)种质资源及其种质创新利用[J].中国农业科技导报,2000,2(6):69－72.
[8]左华清,王子顺.柑桔根际土壤微生物种群动态及根际效应的研究[J].生态农业研究,1995,3:39－47.
[9]罗海峰,齐鸿雁,薛凯,等.PCR-DGGE技术在农田土壤微生物多样性研究中的应用[J].生态学报,2003,23(8):1570－1575.
[10]李华荣,肖建国,颜思齐.蜡质芽孢杆菌R2防治水稻纹枯病研究[J].植物病理学报,1993,23(2):101－105.
[11]Bernadetle M Duineveld, George A Kowalchuk, Anneke Keijzer, et al. Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA as well as DNA fragments coding for rRNA[J]. Applied and Environmental Microbiology, 2001,67:172－178.
[12]Ching-hong Yang,Davide Crowle Y. Rhizosphere microbial community structure in relation to root location and plant iron nutritional status[J].Applied and Environmental Microbiology, 2000,66:345－351.

相似文献/References:

[1]高越,刘辉,陶波.抗草甘膦野生大豆筛选及其抗性生理机制研究[J].大豆科学,2013,32(01):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
　GAO Yue,LIU Hui,TAO Bo.Screening and Physiological Mechanisms of Resistance to Glyphosate in Wild Soybeans(Glycine soja)[J].Soybean Science,2013,32(02):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
[2]王军卫,侯立江,李? 登,等.野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al.Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(02):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
[3]王军卫,侯立江,李 登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(02):596.
[4]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
　WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(02):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[5]陈丽丽,王明玖,何丽君,等.野生大豆ISSR体系的优化及其在远缘杂交后代鉴定中的利用[J].大豆科学,2013,32(04):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
　CHEN Li-li,WANG Ming-jiu,HE Li-jun,et al.Optimization for ISSR Reaction System of Wild Soybean and Its Utilization in Distant Hybrid Identification[J].Soybean Science,2013,32(02):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
[6]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
　ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(02):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[7]徐艳平,胡翠美,张文会,等.干旱胁迫对野生大豆幼苗光合作用相关指标的影响[J].大豆科学,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
　XU Yan-ping,HU Cui-mei,ZHANG Wen-hui,et al.Effect of Simulated Drought Stress on Photosynthesis Related Indexes at Seedling Stage of Wild Soybeans[J].Soybean Science,2013,32(02):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
[8]胡卫静,何丽君,何劲莉,等.NaCl胁迫对野生与栽培大豆杂交后代株系生理指标的影响[J].大豆科学,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
　HU Wei-jing,HE Li-jun,HE Jin-li,et al.Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max × Glycine soja[J].Soybean Science,2013,32(02):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
[9]王 旻,梁 玉,王欣欣,等.即墨野生大豆主要成分及其营养价值分析[J].大豆科学,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
　WANG Min,LIANG Yu,WANG Xin-xin,et al.Assessment on Nutritional Compositions and Value of Jimo Wild Soybean[J].Soybean Science,2013,32(02):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
[10]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶（POD）活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
　CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]

备注/Memo

基金项目：黑龙江省农科院青年基金项目（2007年）

作者简介：刘昭军（1974－），男，副研究员，博士，主要从事作物基因工程及分子育种研究。

更新日期/Last Update: 2014-10-21