[1]阮洪生 　葛文中 　安红波.高效液相色谱法测定豆粕中染料木素和大豆苷元的含量*[J].大豆科学,2006,25(04):461-463.[doi:10.11861/j.issn.1000-9841.2006.04.0461]
　Ruan Hongsheng Ge Wenzhong An Hongbo.DETERMINATION OF GENISTEIN AND DAIDZEIN IN SOYBEAN RESIDUE BY HPLC[J].Soybean Science,2006,25(04):461-463.[doi:10.11861/j.issn.1000-9841.2006.04.0461]
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高效液相色谱法测定豆粕中染料木素和大豆苷元的含量*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年04期 页码: 461-463 栏目: 出版日期: 2006-11-25
Title:
DETERMINATION OF GENISTEIN AND DAIDZEIN IN SOYBEAN RESIDUE BY HPLC
作者:
阮洪生 　葛文中 　安红波
(黑龙江八一农垦大学生命科技学院 ,大庆 163319)
Author(s):
Ruan Hongsheng 　Ge Wenzhong 　An Hongbo
(Li f e Science and Technology College , HLJ August First Land Reclamation University ,Daqing 163319)
关键词:
豆粕; 染料木素 ; 大豆苷元 ; 高效液相色谱法
Keywords:
Soybean residue ; Genistein; Daidzein; HPLC
DOI:
10.11861/j.issn.1000-9841.2006.04.0461
文献标志码:
A
摘要:
　采用高效液相色谱法测定豆粕中染料木素和大豆苷元的含量 。色谱条件:十八烷基键合硅胶柱 ;流动相:甲醇—水(6:4);流速 :0. 80 mL /min;检测波长:260 nm 。结果本方法操作简便、快速、准确,可用于豆粕中染料木素和大豆苷元的质量控制方法。
Abstract:
　To establish the determination method of Genistein and Daidzein in soybean residue , HPLC wasadopted. In this method ODS C18 column was used, methanol-water (6:4)was used as a mobile phase.The detection wave-length was at 260nm. T his method is simple and the result is reliable.

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备注/Memo

课题来源:黑龙江省大庆市科研课题:200294

作者简介:阮洪生(1973 -), 男, 硕士, 讲师, 主要从事中药学的科研和教学工作。
致谢:黑龙江八一农垦大学生命科技学院 2002 级毛越、王海燕、马丽红同学参加了部分实验工作,在此表示感谢

更新日期/Last Update: 2016-12-16