[1]邵红涛 　 许艳丽 *.木霉菌(胞外水解酶)拮抗大豆根腐病病原菌的机制研究[J].大豆科学,2006,25(04):429-433.[doi:10.11861/j.issn.1000-9841.2006.04.0429]
　Shao Hongtao Xu Yanli *.Assessment of Production of Extracellular Enzymesby Trichoderma spp. for Control of SoybeanRoot Rot Pathogens (Fusarium oxysporum ,Rhizoctonia solani)*[J].Soybean Science,2006,25(04):429-433.[doi:10.11861/j.issn.1000-9841.2006.04.0429]
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木霉菌(胞外水解酶)拮抗大豆根腐病病原菌的机制研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年04期 页码: 429-433 栏目: 出版日期: 2006-11-25
Title:
Assessment of Production of Extracellular Enzymesby Trichoderma spp. for Control of SoybeanRoot Rot Pathogens (Fusarium oxysporum ,Rhizoctonia solani)*
作者:
邵红涛 1 　 许艳丽 2*
(1. 黑龙江大学农学院 ,哈尔滨 150080;2. 中国科学院东北地理与农业生态研究所, 哈尔滨 150040)
Author(s):
Shao Hongtao 1 　 Xu Yanli 2*
(1. College of Agriculture , Heilongjiang University , Habin 150080;2. Northeast Institute ofGeography and Agricultural Ecology , Chinese Academy of Sciences, Habin 150040)
关键词:
木霉菌 ; 大豆根腐病 ; 机制 ; 胞外酶; 几丁质酶 ; β-1;  3-葡聚糖酶
Keywords:
Trichoderma spp. ; Root rot of soybean; Mechanism ; Extracellular enzyme;  Chiti-nase ; β- 1; 3 - glucanase
DOI:
10.11861/j.issn.1000-9841.2006.04.0429
文献标志码:
A
摘要:
通过室内试验与温室试验研究了具有生防能力的木霉菌株 Trichoderma MM35 所分泌的胞外水解酶在拮抗大豆根腐病病原菌(F. oxysporum 、R. solani)中的作用 。试验结果表明:以病原菌 F. oxysporum烘干的菌丝体作唯一碳源 ,可以诱导 Trichoderma MM35 分泌几丁质酶、β-1, 3-葡聚糖酶。β-1 ,3-葡聚糖酶高水平诱导表达在前 ,几丁质酶诱导表达在后。土壤中接种 Trichoderma MM35 、F. oxysporum 和 R. so-lani 之后都能够检测到几丁质酶、β-1, 3-葡聚糖酶活性 。向有病原菌 F. oxysporum 的土壤中接种 Tri-choderma MM35,土壤中几丁质酶活性能够显著升高 。向有病原菌 R. solani 的土壤中接种 TrichodermaMM35, 土壤中的几丁质酶、β-1,3-葡聚糖酶活性都显著升高。
Abstract:
The role of extracellular enzymes by Trichoderma MM35 for control of soybean rootrot pathogens(Fusarium oxysporum , Rhizoctonia solani) was assessed invitro and in vivo. De-tective levels of hydrolytic extracellular enzymes were recorded by Trichoderma MM35 usingdried F. oxysporum mycelium as C-source in vitro or fresh F. oxysporum mycelium or fresh R.solani mycelium in vivo was found that there were significant increases in chitinase activities byTrichoderma MM35 in soil with inoculation of F. oxysporum. Soil infested with TrichodermaMM35 had significantly elevated chitinase and β - 1, 3- glucanase activities in presence of R. so-lani as compared to R. solani control.

参考文献/References:

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2 　 Yedidia, I. , Benhamou, N. , Kapulnik, Y. , et al. Inductionand accumulation of PR proteins activity during early stages ofroot colonization by the mycoparasite Trichoderma harzianumstrain T-203[ J] . Plant Physiol. Biochem. 2000, (38):863 -873.
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备注/Memo

基金项目:黑龙江省重点项目(GB01B201);黑龙江大学青年教师基金项目(QL200445)作者简介:邵红涛(1978 -), 男, 助教, 从事土壤微生物学教学, E - mail:shaohongtao2003@yahoo. com. cn

更新日期/Last Update: 2016-12-16