[1]张 　 莉 　 汪东风 　 张 　 宾 　 孙丽平.豆类植物蛋白酶抑制剂研究进展＊[J].大豆科学,2006,25(03):314-319.[doi:10.11861/j.issn.1000-9841.2006.03.0314]
　Zhang Li Wang Dongfeng Zhang Bin Sun Liping.ADVANCES IN THE STUDY OF PROTEASE INHIBITORS FROM LEGUMIN OUS PLANTS[J].Soybean Science,2006,25(03):314-319.[doi:10.11861/j.issn.1000-9841.2006.03.0314]
点击复制

豆类植物蛋白酶抑制剂研究进展＊

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年03期 页码: 314-319 栏目: 出版日期: 2006-08-25
Title:
ADVANCES IN THE STUDY OF PROTEASE INHIBITORS FROM LEGUMIN OUS PLANTS
作者:
张 　 莉 　 汪东风 　 张 　 宾 　 孙丽平
(中国海洋大学食品科学与工程学院 ,青岛 266003)
Author(s):
Zhang Li 　 Wang Dongfeng 　 Zhang Bin 　 Sun Liping
(College of Food Science and Engineering , Ocean University of China , Qingdao 266003)
关键词:
豆类植物; 丝氨酸蛋白酶抑制剂; 半胱氨酸蛋白酶抑制剂
Keywords:
Leguminous plants; Serine proteinase inhibitor; Cysteine proteinase inhibitor
DOI:
10.11861/j.issn.1000-9841.2006.03.0314
文献标志码:
A
摘要:
对国内外豆类丝氨酸蛋白酶抑制剂和半胱氨酸蛋白酶抑制剂的结构 、理化性质和生理功能进行了阐述 ,探讨了在抗虫植物基因工程和临床上的应用。
Abstract:
Protease inhibitors are important in the proteolysis process of plant .It can mediate the degrada-tion of storage proteins for the assimilation of nitrogen into biosynthetic pathways.And it plays an impor-tant role in the plant defense response to insect or pathogen predation .Protease inhibitor (PI)is generallycategorized into four types.T he intent of the present review is to focus on the structural , physicochemicalcharacteristics and physiological functions of leguminous serine protease inhibitor and cysteine protease in-hibitor .The clinical application and application in resisting pest genetic engineering were also discussed .

参考文献/References:

1　 刘会香 , 张星耀.植物蛋白酶抑制剂及其在林木抗虫基因工程中的应用[ J] .林业科学 , 2005 , 41(3):150 - 157.

2 　Wolfram B., Robort H .Natural protein proteinase inhibitorsand their interaction with proteinaes[ J] .Eur J Biochem, 1992 ,204 :433 -451.
3　 庄炳昌.中国野生大豆生物学研究[ M] .北京:科学出版社,1999, 144 -146.
4　 Kunitz M .Crytallization of a trypsin inhibitor from soybean[ J] .Science , 1945, 101(2635):668 - 669.
5 　 Roychaudhuri R., Sarath G ., Zeece M ., et al.Reversible de-naturation of the soybean Kunitz trypsin inhibitor[ J] .Arch Bio-chem Biophys , 2003 , 412 :20 - 26.
6　 忻骅, 曹凯鸣, 谢可方, 等.大豆 Kunitz 型胰蛋白酶抑制剂新类型Tid 的全序列分析[ J] .中国生物化学与分子生物学学报, 1999,15(4):671 - 673.
7　 李严, 宗晖, 黄小莺, 等.中国野生大豆胰蛋白酶抑制剂的初步研究[ J] .复旦学报(自然科学版), 1999 , 38(5):529 - 532.
8　 Koide T ., T sunasawa S., Ikenaka T .Studies on soybean try psininhibitors and amino acid sequence around the reactive site of soy-bean trypsin inhibitor(Kunitz)[ J] .Eur.J .Biochem , 1993 , 32 :408-416.
9　 Birk Y.T he Bowman-birk inhibitor, ty psin and chymotrypsin in-hibitor from soybeans[ J] .Int J Pept Protein Res , 1985 , 25(2):113 - 131.
10 Odani S., Ikenaka T .Studies on soybean try psin-inhibitors 8 di-sulfide bridges in soybean bowman-birk proteinase inhibitor[ J] .JBiochem, 1973, 74(4):697-715.
11 Wu Y .V ., Sassa D .J.Confo rmation of bowman-birk inhibitor[ J] .Agric Biol Chem, 1994, 45:2863-2868.
12 Brauer A .B., Domingo G .J., Cooke R.M ., et al .A conserved cispeptide bond is necessary for the activity of Bowman-birk inhibi-tor protein[ J] .Biochemistry , 2002, 41(34):10608-10615.
13 Ware J.H., Wan X .S ., Rubin H ., et al .Soybean Bowman-birkprotease inhibitor is a highly effective inhibitor of human mastcell chymase[ J] .A rch Biochem Biophys, 1997, 344(1):133 -138.
14 Struthers B.J., MacDonald J.R.Comparative inhibition of tryp-sins from several species by soybean trypsin inhibitors[ J] .J Nu-tr, 1983, 113(4):800-804.
15 Brandon D.L., Bates A .H ., Friedman M .ELISA analy sis ofsoybean trypsin inhibitors in processed foods[ J] .Adv Ex p MedBiol, 1991, 289:321-337.
16 Sessa J.D ., Wolf J.W .Bowman-birk inhibitors in soybean seedcoats[ J] .Ind Crops Prod., 2001, 14:73-83.
17 Pusztai A .The isolation of two proteins, glycoprotein I and atrypsin inhibitor, from the seeds of kidney bean (Phaseolus vul-garis)[ J] .Biochem J, 1966, 101:379-384.
18 Jacob R.T ., Pattabiraman T .N .Natural plant enzyme inhibitors :Isolation and properties of a try psin/chymotrypsin inhibitor fromkidney bean (Phaseolus vulgaris)[ J] .Indian J Biochem Biophys ,1986, 23:105-109.
19 Wu C., Whitaker R.J .Purification and partial characterizationof four trypsin/chymotrypsin inhibitors from red kidney beans(Phaseolus vulgaris, var.Linden)[ J] .J Ag ric Food Chem.,1990, 38:1523-1529.
20 Gibbs B.F ., Alli I.Characterization of a purified α-amylase inhibi-tor from white kidney beans (Phaseolus vulgaris)[ J] .Food ResInt , 1998, 31(3):217-225.
21 O sman M .A ., Reid P .M ., Weber C .W .T hermal inactivation oftepary bean (Phaseolus acutifolius), soybean and lima bean pro-tease inhibitors:effect of acidic and basic pH[ J] .Food Chem ,2002, 78:419-423.
22 Campos J .E., Whitaker J.R., Yip T .T ., et al.Unusual structur-al characteristics and complete amino acid sequence of a proteaseinhibitor from Phaseolus acutifolius seeds[ J] .Plant PhysiolBioch, 2004, 42:209-214.
23 Abe K ., Kondo H., Watanabe H ., et al Oryzacy statins as thefirst well-defined cy statins of plant origin and their target pro-teinases in rice seeds[ J] .Biomed Biochim Acta , 1991, 50:4-6.
24 Barrett A .J.T he cystatins:a new class of peptidase inhibitors[ J] .Trends Biochem Sci ., 1987, 12:193-196.
25 Esnard F., Esnard A ., Faucher D., et al.Rat cystatin C:thecomplete amino acid sequence reveals a site for N-glycosylation[ J] .Biol Chem Hoppe Seyler, 1990, 371(Suppl):161-166.
26 Abrahamson M ., Alvarez-Fernandez M ., Nathanson C.M .Cy st-atins[ J] .Biochem Soc Symp, 2003, 70:179-199.
27 Abe M ., Abe K ., Komoda M ..Corn kernel cysteine proteinaseinhibitor as a novel cystatin superfamily member of a plant o rigin[ J] .Eur J Biochem , 1992, 209:933-937.
28 Misaka T ., Kuroda M ., Iwabuchi K ., et al .Soyacy statin, a novelcysteine proteinase inhibitor in soy bean, is distinct in proteinstructure and gene organization from other cy statins of animaland plant origin[ J] .Eur J Biochem, 1996, 240:609-614.
29 Learmonth E.M .The influence of soy a flour on bread doughs.Ⅲ .T he distribution of the papain-inhibiting factor in soyabeans[ J] .J Sci .Food Ag ric , 1958, 9:269-273.
30 Brzin J., Ritonja A ., Popovic T ., et al .Low molecular mass pro-tein inhibitor of cysteine proteinases from soybean[ J] .Biol ChemHoppe-Seyler Suppl, 1990, 371:167-170.
31 Hines M .E., Osuala C.I., Nielsen S .S.Isolation and partialcharacterization of a soybean cy statin cy steine proteinase inhibi-tor of coleopteran digestive proteolytic activity[ J] .J Agric.FoodChem, 1991, 39:1515-1520.
32 Brzin J., Popoviě T ., Ritonja A ., et al.Related cystatin inhibi-tors from leaf and from seed Phaseolus vulgaris L[ J] .Plant Sci .,1998, 138:17-26.
33 Mickel C.E., Standiah J., Mich J.Susceptibility of processedsoy flour and soy g rits in storage to attack by T ribolium castane-um[ J] .Agric Exp tech Bull , 1947, 178.
34 Lipke H., Fraenkel G .S., Liener I.E.Effect of soybean inhibi-tor on the growth of T ribolium confusum[ J] .J Ag r Food Chem ,1954 , 2 :410 - 414.
35 谢可方, 董爱武, 忻骅, 等.大豆K UNITZ 型胰蛋白酶抑制剂的稳定性及抗虫性研究[ J] .复旦学报(自然科学版), 2002 , 41(6):631 - 634.
36 Ryan C.A .Protease inhibitors in plant :genes for improving de-fenses against insects and pathogens[ J] .Annu Rev Phytopathol ,1990, 28:425-449.
37 郝贵霞, 朱祯, 朱之悌.豇豆蛋白酶抑制剂基因转化毛白杨的研究[ J] .植物学报, 1999, 41(12):1276-1282.
38 师校欣, 王斌, 杜国强, 等.根癌农杆菌介导豇豆胰蛋白酶抑制剂基因转化苹果主栽品种[ J] .园艺学报, 2000, 27(4):282-284.
39 高越峰, 朱祯, 肖桂芳, 等.大豆 Kunitz 型胰蛋白酶抑制剂基因的分离及其在抗虫植物基因工程中的应用[ J] .植物学报, 1998, 40(5):405-411.
40 Confalonieri M ., Allegro G ., Balestrazzi A ., et al.Regenera-tion of populus nigra transgenic plants expressing a Kunitz pro-teins inhibitor (K ti3)gene[ J] .Mol Breed, 1998, 4:137-145.
41 Schuler T .H., Poppy G.M ., Kerry B.R., et al.Insect-resistanttransgenic plants[ J] .T IBTECH , 1998, 16(4):168-175.
42 蔡步林, 李卓荣.人类免疫缺陷病毒蛋白酶抑制剂的研究开发与展望[ J] .中国生化药物杂志, 2005, 26(3):179-182.
43 Ye X.Y ., Wang H.X ., Ng T .B.Structurally dissimilar proteinswith antiviral and antifungal potency from cowpea (Vig na un-guiculata)seeds[ J] .Life Sci, 2000, 67:3199-3207.
44 Kennedy A.R.Chemopreventive Agents:Protease inhibitors[ J] .Pharmacol Ther, 1998, 78(3):167-209.
45 Yavelow J ., Collinst M ., Birko Y ., et al.Nanomolar concentra-tions of Bowman-Birk soybean protease inhibitor suppress x-ray-induced transformation in vitro[ J] .Proc.Natl.Acad.Sci .USA ,1985, 82:5395-5399.
46 Kennedy , A .R.T he conditions fo r the modification of radiationtransformation in vitro by a tumor promoter and protease inhibi-tors[ J] .Carcinogenesis, 1985, 6:1441-1445.
47 Augustine O .F ., Ashutosh P .B.Long-term feeding of field beanprotein containing protease inhibitors suppresses virus-inducedmammary tumors in mice[ J] .Cancer Lett, 1997, 116:1-7.
48 Augustine O .F ., Ashutosh P .B.The field bean protease inhibi-tor can effectively suppress 7, 12-dimethylbenz[ α] anthracene-in-duced skin tumorigenesis in mice[ J] .Cancer Lett , 1996, 104:219-224.
49 Ashutosh B., Augustine F ., Sanjay B.T reatment with field beanprotease inhibitor can effectively repress ethylnitrosourea(ENU)-induced neoplasms of the nervous system in Sprague-dawley rats[ J] .Cancer Lett, 1998, 130:161-167.
50 Ashutosh B., Augustine F ., Sanjay B., et al.The field bean pro-tease inhibitor has the potential to suppress B16F10 melanomacell lung metastasis in mice[ J] .Cancer Lett , 1998, 129:15-20.
51 GarcíaGasca T ., Salazar Olivo L .A ., Mendiola Olaya E., et al .T he effects of a protease inhibitor fraction from tepary bean(Phaseolus acutifolius)on in vitro cell proliferation and cell adhe-sion of transformed cells[ J] .T oxicol in Vitro , 2002, 16:229 -233.

备注/Memo

基金项目:山东省自然科学基金重点项目(Z2004D05)

作者简介:张莉(1979-), 女, 博士, 研究方向生物化学。E-mail :qdzhang li@ouc.edu.cn
通讯作者:汪东风, E-mail:wangdf @ouc.edu.cn

更新日期/Last Update: 2016-12-16