[1]魏梅生相 　 宁　 张春泉　 Said A. Ghabrial.菜豆荚斑驳病毒的 RT - PCR 检测*[J].大豆科学,2005,24(04):317-319.[doi:10.11861/j.issn.1000-9841.2005.04.0317]
　Wei Meisheng Xiang Ning Zhang Chunquan Said A. Ghabrial.DETECTION OF BEAN POD MOTTLE VIRUS BY RT- PCR[J].Soybean Science,2005,24(04):317-319.[doi:10.11861/j.issn.1000-9841.2005.04.0317]
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菜豆荚斑驳病毒的 RT - PCR 检测*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年04期 页码: 317-319 栏目: 出版日期: 2005-11-25
Title:
DETECTION OF BEAN POD MOTTLE VIRUS BY RT- PCR
作者:
魏梅生1相 　 宁1　 张春泉2　 Said A. Ghabrial2
(1. 中国检验检疫科学研究院动植物检疫研究所 ,北京 100029;2. University of Kentucky)
Author(s):
Wei Meisheng1　 Xiang Ning1　 Zhang Chunquan2　 Said A. Ghabrial2
(1. Institute of Animal and Plant Quarantine , Chinese Academy of Inspection and Quarantine ,Beijing 100029;2.University of Kentucky)
关键词:
菜豆荚斑驳病毒 ; 聚合酶链式反应; 酶联免疫吸附测定
Keywords:
Bean pod mottle virus ; PCR; ELISA
DOI:
10.11861/j.issn.1000-9841.2005.04.0317
文献标志码:
A
摘要:
菜豆荚斑驳病毒是对大豆经济具有重要影响的病毒 。根据已报道的菜豆荚斑驳病毒外壳蛋白基因序列 ,设计出一对特异性引物 ,采用 RT - PCR法 ,可有效地扩增出 BPMV 不同亚组的 4 个分离物,得到 650 bp 大小的 PCR产物。这种方法能成功地检测出大豆病叶中的 BPMV ,结果和酶联检测是一致的 ,而对大豆种皮中 BPMV 的检测效果不佳 ,建议采用酶联的方法进行检测。
Abstract:
Bean pod mottle virus is an economically important virus that infect soybean. A pair of specificprimers was designed based on the conserved region of the coat protein gene of bean pod mottle virus. To-tal RNA was extracted from infected soybean leaves and was reverse transcribed to cDNA. The cDNA wassubjected to PCR using the forward and reverse primers. PCR products of 650 bp were generated from allisolates tested. The RT - PCR method could be used to detect BPMV - infected soybean leaves with satis-fied results that were confirmed by DAS - ELISA assay. We recommended ELISA assay to detect BPMV -infected soybean seed rather than RT - PCR .

参考文献/References:

1　 Gu, H. , Clark, A. J. , de Sa , P. B. , et al. Diversity among iso-lates of Bean pod mottle virus[ J] . Phytopathology , 2002, 92:446- 452.

2　 Giesler, L. J. ,Ghabrial S. A. , Hunt, T. E. , et al. Bean pod mot-tle virus a threat to U. S. soybean production[ J] . Plant Disease ,2002, 86:1280- 1289.
3　 Michelutti R ;Tu JC;Hunt DWA , et al . Anderson First report ofbean pod mottle virus in soybean in Canada[ J] . Plant - Disease ,2002, 86(3):330.
4　 Anjos JRN ;Brioso PST ;Charchar MJA. Partial characterizationof bean pod mottle virus in soybeans in Brazil[ J] . Fitopatologia -Brasileira, 1999, 24(1):85- 87.
5　 Fribourg CE;Perez W . Bean pod mottle virus (BPMV) affect-ing Glycine max (L.) Merr. in the Peruvian jungle[ J] . Fitopato-logia, 1994, 29(3):207- 210
6　 Zettler FW;Stansly PA ;Elliott - M S;et al. Repo rt of bean podmottle virus in South America[ J] . Plant - Disease, 1989, 73(6):518.

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备注/Memo

项目来源:本研究得到国家质量监督检验检疫总局的经费支持(2002 IK015 - 01 ), 特此致谢。　 作者简介:魏梅生(1965 -), 男, 副研究员, 从事植物病毒病害的检疫检测研究。E - mail:wmsh02@yahoo. com. cn

更新日期/Last Update: 2017-01-13