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微信公众号：大豆科学

[1]王延伟　智海剑＊＊　郭东全　盖钧镒＊＊　陈庆山李　凯　李海朝.中国北方春大豆区大豆花叶病毒株系的鉴定与分布＊[J].大豆科学,2005,24(04):263-265.[doi:10.11861/j.issn.1000-9841.2005.04.0263]
　Wang Yanwei Zhi Haijian Guo Dongquan Gai Junyi Chen Qingshan Li Kai Li Haichao.CLASSIFICATION AND DISTRIBUTION OF STRAIN GROUPS OF SOYBEAN MOSAICVIRUS IN NORTHERN CHINA SPRING PLANTING SOYBEAN REGION[J].Soybean Science,2005,24(04):263-265.[doi:10.11861/j.issn.1000-9841.2005.04.0263]

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中国北方春大豆区大豆花叶病毒株系的鉴定与分布＊

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷期数: 2005年04期页码: 263-265 栏目: 出版日期: 2005-11-25

Title:: CLASSIFICATION AND DISTRIBUTION OF STRAIN GROUPS OF SOYBEAN MOSAICVIRUS IN NORTHERN CHINA SPRING PLANTING SOYBEAN REGION

作者:: 王延伟¹　智海剑^1＊＊　郭东全¹　盖钧镒^1＊＊　陈庆山²李　凯 ¹ 　李海朝¹; (1.南京农业大学大豆研究所 ,作物遗传与种质创新国家重点实验室 , 南京 210095;2 .东北农业大学大豆研究所,哈尔滨 150030)

Author(s):: Wang Yanwei ¹ 　 Zhi Haijian ¹　 Guo Dongquan ¹ 　 Gai Junyi ¹ Chen Qingshan ²　 Li Kai ¹　 Li Haichao ¹; (1.Soybean Research Institute , Nanjing Agricultural University , National Key Laboratory forCrop Genetics and Germplasm Enhancement , Nanjing 210095 ;2.Soybean Research Institute ,Northeast Agricultural University , Harbin 150030)

关键词:: 大豆花叶病毒; 鉴别寄主 ; 株系群; 分布

Keywords:: Soybean mosaic virus; Differential hosts; Strain groups ; Distribution of strain groups

DOI:: 10.11861/j.issn.1000-9841.2005.04.0263

文献标志码:: A

摘要:: 从我国北方春大豆区的黑龙江、吉林、辽宁、内蒙、河北承德以及北京采集典型症状的大豆叶片病样,经 Top -Crop 离体叶单斑分离以及 DAS -ELISA 抗血清免疫鉴定, 共获得 112 个 SMV阳性反应分离物。根据 112 个分离物在合丰 25、文丰 5 号、诱变 30、8101、铁丰 25、Davis 、Buffalo 、早熟 18 、广吉、科丰 1号、齐黄 22 共 11 个鉴别寄主上的症状反应, 将我国北方春大豆区的 SMV 分成8 个株系群。各株系群所占比例分析表明,北方春大豆区以 SC-11 株系群为主, 其次为SC -8,各占病样总数的比例分别为51.7%和17.9%,SC -11 和 SC-8 分别在黑龙江和辽宁分布最广,占当地株系群的比例分别为 67.8%和 75.0%。与以往株系划分系统的初步比较表明, SC -11 与东北原 1 号株系群 ,SC -12 和 SC -16 与东北原 2 号株系群 ,SC -4、SC -7、SC -8、SC -13 和 SC -17 与东北原 3 号株系群在鉴别寄主上的反应相似。SC -16 和 SC -17 为 2 个新发现的株系群。

Abstract:: 904 specimens of soybean mosaic virus (SMV)were collected from Heilongjiang , Jilin, Liaon-ing , Hebei , Neimeng and Beijing in Northern China Spring Planting Soybean Region .The symptomatolo-gy and serology were carried out to purify and detect SMV .From them ,112 SMV isolates were obtained.Based on their response to 11 differential hosts such as Hefeng 25, Wenfeng 5, Youbian 30, 8101, Tiefeng25, Davis, Buffalo , Zaoshu 18, Kwanggyo , Kefeng 1 and Qihuang 22, 112 SMV isolates were classifiedinto 8 strain groups.Among them , SC -11 was the widest in Northern China Spring Planting Soybean Re-gion with a proportion of 51.7%, and then SC-8, with a proportion of 17.9%.As far as the proportionof local SMV strain groups are concerned , the distributions of SC -11 and SC -8 were the widest in Hei-longjiang with a proportion of 67.8%and in Liaoning with a proportion of 75.0 %, respectively .The re-sults of comparison of 11 SMV strain groups in present experiment with strain groups identified by LuWenqing showed that , SC -11 was similar with No .1 strain groups, SC -12 and SC -16 were similar withNo .2 , SC -4,SC -7, SC -8,SC -13 and SC -17 were similar with No .3.SC -16 and SC -17 were newstrain groups.

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备注/Memo

　基金项目:国家自然科学基金资助(30170607)　作者简介:王延伟(1979-)男, 硕士研究生, 研究方向大豆抗病遗传育种。　＊＊联系作者 Tel:025-84396463, 025-84395405, E-mail:zhj @njau .edu.cn;sri@njau.edu.cn

更新日期/Last Update: 2017-01-08