[1]胡卫新　 王晓磊 *张 　 洁.大豆异黄酮提取条件和大豆蛋白质分离工艺研究*[J].大豆科学,2005,24(01):26-29.[doi:10.11861/j.issn.1000-9841.2005.01.0026]
　Hu Weixin Wang Xiaolei * Zhang Jie.STUDY ON THE CONDITIONS OF EXTRACTINGSOYBEANISOFLAVONES AND SEPARATING PROTEIN[J].Soybean Science,2005,24(01):26-29.[doi:10.11861/j.issn.1000-9841.2005.01.0026]
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大豆异黄酮提取条件和大豆蛋白质分离工艺研究*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年01期 页码: 26-29 栏目: 出版日期: 2005-02-25
Title:
STUDY ON THE CONDITIONS OF EXTRACTINGSOYBEANISOFLAVONES AND SEPARATING PROTEIN
作者:
胡卫新1　 王晓磊2 *张 　 洁3
(1. 中国矿业大学化工学院, 江苏 徐州 221008;2. 上海旭龙农业科技有限公司 ,上海 200063 ;3. 中国矿业大学环测学院,江苏 徐州 221008)
Author(s):
Hu Weixin 1 　 Wang Xiaolei 2 * 　 Zhang Jie 3
(1.School of Chemical Engineering and Technology , CUMT , Xuzhou , 221008;2.Shanghai Xulong Agriculture - technology Ltd. Shanghai 200063 ;3.School ofEnvironment Science and Spatial Inf ormatics , CUMT , X uzhou, 221008)
关键词:
豆粕; 大豆异黄酮; 蛋白质; HPLC
Keywords:
Soybean meal ; Soybean isoflavones; Protein; HPLC
DOI:
10.11861/j.issn.1000-9841.2005.01.0026
文献标志码:
A
摘要:
以豆粕为原料 ,以总大豆异黄酮为目标产物 ,用正交试验设计研究了用乙醇提取大豆异黄酮的较优条件 ,70 %(V /V)乙醇 ,固液比(豆粕 ∶溶剂)1 ∶10(W /V),70 ℃下提取 3 次, 每次回流时间1. 5h 。在室温下, 采用盐析和等电点沉淀工艺分离除去提取液中的大豆蛋白质。试验表明, pH 值在 4. 1±0. 1 下 ,加入 3. 0%(m /v)的 ZnCl 2 ,大豆蛋白质的分离除去率最高。
Abstract:
The conditions of extracting soybean isoflavones from soybean meal with ethanol was proposedand investigated in this paper. On the purpose to get the yield of total soybean isoflavones as high as possi-ble , with orthogonal experimental methodology , the optimum parameters of extracting isoflavones wereobtained , i. e. 70 %(V /V)ethanol , soybean meal weight to solvent volume at 1 ∶10 , temperature 70℃,time 1. 5h and 3 times repeat. The protein was separated from the pre - extracting liquid with salting - outand isoelectric precipitation at room temperature. The results indicated that the separation quotiety of pro-tein was the highest with the ZnCl 2 (3. 0%,m /v)at pH 4. 1±0. 1.

参考文献/References:

1　 Michael Naim, Shmuel Zilkah. Soybean Isoflavones. Character-ization, Determination and Autifungal Activity[ J] . Food Chem ,1994, 22(5):806 - 810.

2　 郑高利. 大豆异黄酮的药理作用[ J] . 中国现代应用药学, 1998, 15(1):4- 11.
3　 K. R. 马卡姆. 黄酮类化合物结构鉴定技术[ M] . 北京:科技出版社, 1990. 21- 30.
4　 张龙翔, 张庭芳, 李令媛. 生化实验方法和技术[ M] . 北京:高等教育出版社, 1981, 55 - 59.
5　 黄伟坤. 食品分析与检验[ M] . 北京:中国轻工业出版社, 1989.45.
6 　 Waggle. Recovery of isoflavones from soy molasses [ P ] .US00591919921A.
7　 沈同, 王镜言. 生物化学(上册, 第二版)[ M] . 北京:高等教育出版社, 1990, 197.

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备注/Memo

作者简介:胡卫新(1977 -), 男, 硕士研究生, 主要从事精细化学品分离的研究。电话:0516- 2874282;E - mail:hwx 97@163. com

更新日期/Last Update: 2016-12-23