[1]李春杰 许艳丽 李兆林 邵洪涛 司兆胜.大豆根腐病菌拮抗细菌筛选及抗生作用[J].大豆科学,2004,23(03):174-177.[doi:10.11861/j.issn.1000-9841.2004.03.0174]
Li Chunjie Xu Yanli Li Zhaolin Shao Hongtao.SCREENINGOF ANTAGONISTIC BACTERIA AGAINST PATHOGENIC FUNGUSOF SOYBEAN ROOT ROT AND THEIR BIOCONTROL EFFICIENCY[J].Soybean Science,2004,23(03):174-177.[doi:10.11861/j.issn.1000-9841.2004.03.0174]
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Li Chunjie Xu Yanli Li Zhaolin Shao Hongtao.SCREENINGOF ANTAGONISTIC BACTERIA AGAINST PATHOGENIC FUNGUSOF SOYBEAN ROOT ROT AND THEIR BIOCONTROL EFFICIENCY[J].Soybean Science,2004,23(03):174-177.[doi:10.11861/j.issn.1000-9841.2004.03.0174]
大豆根腐病菌拮抗细菌筛选及抗生作用
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
卷:
第23卷
期数:
2004年03期
页码:
174-177
栏目:
出版日期:
2004-08-25
- Title:
- SCREENINGOF ANTAGONISTIC BACTERIA AGAINST PATHOGENIC FUNGUSOF SOYBEAN ROOT ROT AND THEIR BIOCONTROL EFFICIENCY
- 文章编号:
- 1000 -9841(2004)03 -0174 -04
- 关键词:
- 中国科学院东北地理与农业生态研究所; 哈尔滨150040
- Keywords:
- Soybean root ro t ; Antagonistic bacteria ; Biocontrol ef ficiency
- 分类号:
- S 435.651
- 文献标志码:
- A
- 摘要:
- 从大豆根际土壤中分离纯化得到388 个细菌菌株, 从中筛选对大豆根腐病病原尖孢镰刀菌(Fusarium oxysporum)、茄腐镰刀菌(Fusarium solani)和立枯丝核菌(Rhizoctonia solani )有拮抗作用的菌株, 采用无菌室内点接法初筛, 抑菌带大于20mm 以上的有12 株, 通过盆栽防效试验, 进一步证明12 个菌株中有6 个菌株盆栽防治尖孢镰刀菌和立枯丝核菌达到46 .0 %-78 .5 %。菌株与病原真菌共培养结果显示, 菌株B2 、B57 、B85 和B69 使尖孢镰刀菌菌丝干重分别减少51 .27 %、40 .78 %、38 .87 %和34 .58 %;菌株B57 使茄腐镰刀菌菌丝干重减少71 .48 %。菌株分泌物对病原真菌的拮抗作用研究结果表明, B57 的分泌物对尖孢镰刀菌抑制率最高, 3d 为82 .1 %, 6d 为90 .6 %;其次是B16 , 3d 为58 .8 %, 6d 为51 .1 %。
- Abstract:
- Three hundred and eighty eight bacterial strains were isolated f rom soybean rhizosphere soil .Twelvebacterial st rains w ere screened by the inhibition zone method ,w hich had bet ter eff icacy against the major causalagent s :Fusarium oxysporum , Fusarium solani , Rhizoctonia solani .In pot ted trial , 6 strains show ed some efficacy against pathogenic fungus of soy bean root rot .The dry w eight s of Fusarium oxysporum , Fusarium solaniw ere reduced 40 .78 %and 71 .48 %respect ively with w hich the strain B57 w as cocultured in King’ s broth(KB)liquid medium .The radius of pathogenic fungi(Fusarium oxysporum )w as reduced respectively 82 .1 % in 3dand 90 .6 %in 6d by the diffusion of strain B57 compared with that of the control .In general , the test s in vitroand in vivo show ed that st rain B57 had the best biological cont rol ef ficacy against pathogens of soybean root rot
参考文献/References:
1 韩庆新, 辛惠普.大豆根腐病主要病原菌对大豆幼苗致病性的初步研究[ J] .大豆科学, 1990 , 9(2):157 -161 .2 苔莲梅.大豆根腐病菌(F usarium oxysporum )毒素及其对大豆根部致病菌作用的研究[ D] .东北农业大学硕士论文, 2003 .3 方中达.植病研究法[ M] .北京:农业出版社, 1977 , 161 -224 .4 刘世怡, 周瑞荣, 左锐.水稻纹枯病的拮抗细菌筛选[ J] .贵州农业科学, 1995 , 3 :15 -17 .5 陈双雅, 张永祥, 蔡向群.三株拮抗细菌对水仙叶大褐斑病的拮抗机理[ J] .中国生物防治.2003 , 19(1):11 -15 .6 郑爱萍, 李平, 王玲霞, 等.水稻纹枯病拮抗细菌的筛选及抗生性研究[ J] .西南农业学报, 2001 , 14(1):78 -81 .7 彭化贤, 刘云微, 何忠全, 等.水稻稻瘟病拮抗细菌的分离和筛选[ J] .西南农业学报, 1999 , 12(3):85 -88 .
备注/Memo
基金项目:国家“ 十五” 科技攻关项目(2001BA507A05 -02);中国科学院知识创新工程重大项目(KZCX1 -SW-19 -4 -03);黑龙江省重点项目(GB01B201)作者简介:李春杰(1976 -), 女, 研究实习员, 硕士研究生, 从事大豆病虫害生物防治研究。
更新日期/Last Update:
2017-01-01