[1]袁金斌卢建中.紫外分光光度法测定大豆总异黄酮的含量[J].大豆科学,2004,23(02):147-150.[doi:10.11861/j.issn.1000-9841.2004.02.0147]
　Yuan Jinbin Lu Jianzhong.DETERMINATION OF SOYBEAN ISOFLAVONE WITHULTRA-VIOLET SPECTROPHOTOMETRY[J].Soybean Science,2004,23(02):147-150.[doi:10.11861/j.issn.1000-9841.2004.02.0147]
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紫外分光光度法测定大豆总异黄酮的含量

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第23卷 期数: 2004年02期 页码: 147-150 栏目: 出版日期: 2004-05-25
Title:
DETERMINATION OF SOYBEAN ISOFLAVONE WITHULTRA-VIOLET SPECTROPHOTOMETRY
文章编号:
1000 -9841(2004)02 -0147 -04
作者:
袁金斌1卢建中2
1.江西中医学院中药制药研究中心, 南昌330006 ;
2.江中制药集团技术部, 南昌330077
Author(s):
Yuan Jinbin1 　Lu Jianzhong2
1 .Pharmacy Research Cent re for TCM , Jiangxi Universi ty of Tradi tionalChinese Medicine , Nanchang 330006 ;
2 .Technology Department , J iangzhongPharmacy Group , Nanchang 330077
关键词:
大豆异黄酮; 分光光度法; 测定
Keywords:
Soybean isoflavone ; UV spect rophotometry ; Determination
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.02.0147
文献标志码:
A
摘要:
建立了测定大豆提取物及以大豆展品黄酮为原料的保健食品中大豆总异黄酮的紫外分光光度法。以染料木素为标准品, 优选出325nm 为测量波长, 以染料木素计量试样中的大豆总异黄酮。大豆提取物中大豆总异黄酮的含量为38.1 %, 加标回收率为102.3 %;某保健品中大豆总总异黄酮的含量为4.76 %, 加标回收率为99.8 %。方法简便、准确、重现性好, 适用于保健品的日常分析和质量控制。
Abstract:
A UV spect rphotomet ry used fo r determination of soybean isoflavone w as established .Genistein w asselected as the reference material , and the samples were determined at 325 nm .Influencing factors were dicussedand optimized .The total content of soybean isof lavo nes in soybean ex tracts is 38 .1 %w ith mean recovery102 .3 %, and the result for the function food is 4 .76 %w ith mean recovery 99 .9 %.This method is suitable forthe routine analysis and quality control of the funct ion food wi th easy -to -handle , good accuracy and reproducibility.

参考文献/References:

1 　张立.RP-HPLC 法测定大豆提取物中大豆苷元、染料木素、大豆苷、染料木苷的含量[ J] .中草药, 2001 , 32(1):118 -120.

2 　赵世萍, 章育中.异黄酮含量的薄层光密度法测定[ J] .药学学报, 1985 , 20(3):203 -208.
3 　G .S .Mcleod , M .J .S heperd .Determination of the ionization constant s of isof lavones by capillary elect rophoresis[ J] .Czech .J .FoodS ci., 1999 , 17(2):61 -67 .
4 　G .J .Wang, O .Lapcik , R.Hampl , et al.Time-resolved f luoroimmunoassayof plasma daidzein and genistein[ J] .St eroids , 2000 , 65(6):339 -348.
5 　张玉梅, 孙学斌, 高旭年, 等.紫外分光光度法测定大豆总异黄酮的含量[ J] .中国食品卫生杂志, 2000 , 12(4):7 -8.
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备注/Memo

作者简介:袁金斌(1971 -), 男, 理学硕士, 讲师,现从事天然产物活性成分的研究。　E -mai l:kings_008 @yahoo .com .cn

更新日期/Last Update: 2016-12-31