[1]乔亚科　李桂兰　高书国　毕艳娟.冀东地区野生大豆愈伤组织诱导及其耐盐性[J].大豆科学,2002,21(03):208-213.[doi:10.11861/j.issn.1000-9841.2002.03.0208]
　Qiao Yake Li Guilan Gao Shugo Bi Yanjuan.THE STUDY ON SALT -TOLERANCE ABILITY AND INDUCTION OFCALLUS OF WILD SOYBEAN IN EASTERN OF HEBEI PROVINCE[J].Soybean Science,2002,21(03):208-213.[doi:10.11861/j.issn.1000-9841.2002.03.0208]
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冀东地区野生大豆愈伤组织诱导及其耐盐性

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第21卷 期数: 2002年03期 页码: 208-213 栏目: 出版日期: 2002-08-25
Title:
THE STUDY ON SALT -TOLERANCE ABILITY AND INDUCTION OFCALLUS OF WILD SOYBEAN IN EASTERN OF HEBEI PROVINCE
文章编号:
1000 -9841(2002)03 -0208 -06
作者:
乔亚科　李桂兰　高书国　毕艳娟
河北职业技术师范学院植物细胞工程实验室, 昌黎066600
Author(s):
Qiao Yake 　Li Guilan 　Gao Shugo 　Bi Yanjuan
Hebei Vocation -Technical Teachers College , Changli , 066600
关键词:
野生大豆; 愈伤组织; 耐盐性
Keywords:
Wild soybean ; Callus ; Salt -tolerance
分类号:
S565.1 S336
DOI:
10.11861/j.issn.1000-9841.2002.03.0208
文献标志码:
A
摘要:
以冀东沿海及内地不同条件下生长的14 个野生大豆为试验材料, 选取下胚轴和子叶作为外植体, 在四种不同成分的培养基上诱导愈伤组织, 经继代观察愈伤组织的质量。同时, 在含不同浓度NaCl(0 、0 .2 %、0 .4 %、0 .6 %、0 .8 %、1 .0 %)的继代培养基上, 观察愈伤组织的耐盐性。试验结果表明:诱导野生大豆愈伤组织的适宜培养基为MS +2 , 4 -D 2 .0mg/L +6 -BA0 .5 mg/L +蔗糖5 %+琼脂0 .8 %;不同材料之间愈伤组织的耐盐性有明显差异, 耐盐性强的可在0 .8 %NaCl 浓度下生存, 低的只能忍耐0 .2 %NaCl 含量;本试验确定野生大豆愈伤组织耐盐性鉴定适宜的浓度为0 .6 %左右;盐碱条件下生长的野生大豆一般耐盐性较强, 非盐碱环境下生长的野生大豆耐盐性也存在着耐盐性较强的类型, 说明生长环境与细胞水平的耐盐性之间并没有完全的对应关系。
Abstract:
The 14 genoty pes of wild soy bean growing at different condi tions in eastern of Hebei province w erestudied in this paper .Hypocotyls and co tyledon were used as ex plants , through tissue culture , calli w as inducedon 4 kinds of medium containing different composition .The calli were subcultured on D4 medium for 4 times.The salt -tolerance of calli was observed on dif ferent medium containing 0 .0 , 0 .2 , 0 .4 , 0 .6 , 0 .8 , 1 .0 %NaCl.Abili ty of salt -tolerance w as identif ied through g row th amount , quality , becoming brow n and death ratio of calli.The result s show ed that MS +2 , 4 -D2 .0mg/L +6 -BA0 .5mg/L +sucrose 5 %+ag ar 0 .8 % is the bestmedium for callus induction .Salt -tolerance ability of calli had obvious dif ferent among different w ild soybean .The stronger salt -tolerance w ild soybean could survive at the concent ration of 0 .8 %NaCl .The ability of salt -tolerance of 6 、7 、10 、11 、12 、14 w as stronger , the g row th amount and quality of calli descended with the rising ofconcent ration of NaCl.The experiment indicated that screening concent ration fo r salt -tolerance of w ild soybeanis 0 .6 %NaCl.Generally , the w ild soybeans f rom saline soil have st ronger abili ty of salt -tolerance ,w here as,the w ild soybean f rom non -saline environment have very strong existing ability in NaCl medium , too .There w as no t complete co rrespondence relatio n betw een g row th environment and salt -tolerance ability at cell level.The results sug gested that the relation of salt -tolerance ability at cell level and plant level should study further .

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备注/Memo

作者简介:乔亚科(1964 -), 男, 副教授, 从事大豆等遗传资源及育种研究。

更新日期/Last Update: 2016-12-30