[1]孙君明丁安林东惠茹.高效液相色谱( HPLC)技术检测大豆异黄酮含量[J].大豆科学,2000,19(01):15-20.[doi:10.11861/j.issn.1000-9841.2000.01.015]
　Sun JunmingDing AnlinDo ng Hui ru.HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATIONOF ISOFLAVONE CONTENT IN SOYBEAN TEST SAMPLES[J].Soybean Science,2000,19(01):15-20.[doi:10.11861/j.issn.1000-9841.2000.01.015]
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高效液相色谱( HPLC)技术检测大豆异黄酮含量

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第19卷 期数: 2000年01期 页码: 15-20 栏目: 出版日期: 2000-02-25
Title:
HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATIONOF ISOFLAVONE CONTENT IN SOYBEAN TEST SAMPLES
作者:
孙君明1丁安林1东惠茹2
1.中国农业科学院作物育种栽培研究所,北京, 100081
2.中国农业科学院蔬菜花卉研究所,北京, 100081
Author(s):
Sun Junming1　Ding Anlin1　Do ng Hui ru2
1. Inst itute of Crop B reeding and Cult ivat ion , CAA S , Beij ing, 100081
2. Inst itute of Vegetables and Flowers, CA A S, Bei jing , 100081
关键词:
大豆(Glycine max ) ;  高效液相色谱;  异黄酮
Keywords:
Soy bean ( Glycine max ) ;  HPLC;  Isof lav one
DOI:
10.11861/j.issn.1000-9841.2000.01.015
文献标志码:
A
摘要:
利用高效液相色谱( HPLC)技术分离并测定了大豆中异黄酮各组分含量,建立了快速、有效的分离、检测异黄酮含量的方法。结果显示,异黄酮的最适提取时间是2- 3hr; 最适检测波长为260nm; 在一定的浓度范围内,标准样品Daidzin和Geni stin作为异黄酮的检测标样是准确的; 提取和检测温度对异黄酮组合含量有很大影响,在80℃或更高的温度条件下,丙二酰基结合体可水解为相应的甙或甙元的形式。
Abstract:
Soy bean isoflav one compounds w ere separated and determined w ithHPLC, a nd a simple sensi tiv e and hig hly reproducible method wa s established. Theresul t indica ted the mo st suitable ex t racting time of i sof lav ine is 2- 3h r, the sui tableabso rbent w av eleng th is 260nm; It i s accurate for th e iso flavo ne standard sample w ithdaidzin and g enistin at the range of concent ra tion; The i sof lav o ne co ntent is af fected byex t racting and examining temperatures. At 80℃ or higher tempera ture, i sof lav o nemalonyl conjug ates are hydroly zed to glucosides and ag lycones.

参考文献/References:

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2　Huang, A. S. , Hsi eh O. A. L. and Chang, S. S. , Charact eri zati on of th e nonv ol atil e minor cons tituent sresponsible f or th e objecti onab le t as t e of def at t ed soybean f lour. J. of Food Sci. 1981, 47, 19- 23
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6　Murph y, P. A. Separation of genis tin, daidzin and thei r aglucones , and coum es terol by g radient hi gh -perfo rmance liquid ch romatog raphy. J. Ch romat ograph y. 1981, 21, 166- 169
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更新日期/Last Update: 2016-12-27