[1]费甫华　谈宇俊　周茂繁.大豆锈病与过氧化物酶同工酶关系的初步研究[J].大豆科学,1997,16(02):107-112.[doi:10.11861/j.issn.1000-9841.1997.02.0107]
　Fei FuhuaTan YujunZhou Maofan.PRELIMINARY STUDY ON THE CORRELATION BETWEENSOYBEAN RUST INFECTION AND PEROXIDASE ISOENZYMES[J].Soybean Science,1997,16(02):107-112.[doi:10.11861/j.issn.1000-9841.1997.02.0107]
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大豆锈病与过氧化物酶同工酶关系的初步研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第16卷 期数: 1997年02 页码: 107-112 栏目: 出版日期: 1997-05-20
Title:
PRELIMINARY STUDY ON THE CORRELATION BETWEENSOYBEAN RUST INFECTION AND PEROXIDASE ISOENZYMES
作者:
费甫华　谈宇俊1　周茂繁2
(湖北省宜昌市农业科学研究所　宜昌　443004)
Author(s):
Fei Fuhua1　Tan Yujun2　Zhou Maofan3
( 1 Yichang Muni cipalit y Agri cultural Science Research Inst itute, Yichang 443004
2 Oil Crops Research Institute, Chinese Academy of Agricul tural Sciences, Wuhan 430062
3 Depar tment of plant protection, Huazhong Agri cultural Universit y , Wuhan 430070)
关键词:
大豆锈病;  过氧化物酶;  同工酶
Keywords:
Soybean rus t;  Peroxidase;  Isoenzymes
DOI:
10.11861/j.issn.1000-9841.1997.02.0107
文献标志码:
A
摘要:
采用聚丙烯酰胺凝胶电泳,研究了大豆锈病不同严重度、离病斑中心不同距离,以及抗感锈病不同品种接种后病程与过氧化物酶同工酶的关系,结果表明:发病不同严重度(Ⅰ ～ Ⅲ 级)比对照(未接种0级)酶谱带多1～ 2条,活性由大到小依次为Ⅱ 级> Ⅰ 级> Ⅲ 级> 0级; 离病斑中心越近酶带越多,且酶活性越强;随着接种时间的推迟( 0～ 32小时)过氧化物酶带数增多,酶活性增强; 抗病品种与感病品种接种后相同时间进行比较,抗病品种比感病品种多1～ 4条酶带,且其酶活性亦较高; Rf值0. 78～ 0. 85间的酶谱与大豆锈病的发生发展有着密切关系。
Abstract:
Using v ertical g el elect rophoresis, we s tudied the correlation between peroxidase isoenzymeand soybean rust of dif ferent s everit y and different distance apart f rom diseas e spot’ scenter and dif ferent time af ter inoculation in resis tant and susceptible varieties. The resultsshowed that there was one or tw o more isoenzyme pat tern bands in diff erent inf ection s everit y( from Ⅰ to Ⅲ grade) than CK t reatment ( Uninoculated, 0 g rade) . Peroxidase activi ty f romhigh t o low was Ⅱ grade> Ⅰ g rade Ⅲ g rade> 0 grade. The closer dist ance apart f rom diseasespo t’ s center and the period aft er inoculation in resis tant and susceptible varieties themore s tronger activit y and the more enzyme pat tern bands. Comparing resis tant and susceptiblev arieti es at the same time af ter inocula tion, the resis tant one had 1 to 4 more enzyme pattern bands and s t ronger enzyme activit y than sus ceptible one. Besides there was clos e correlationbetween RF value ( 0. 78 to 0. 85) and development of soybean rus t.

参考文献/References:

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更新日期/Last Update: 2017-01-13