[1]康朝锐,韩佳音,杨明亮,等.大豆FBL基因家族鉴定及百粒重相关基因挖掘与单倍型分析[J].大豆科学,2026,(04):35-45.[doi:10.11861/j.issn.1000-9841.2026.04.0035]

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大豆FBL基因家族鉴定及百粒重相关基因挖掘与单倍型分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 期数: 2026年04期页码: 35-45 栏目: 出版日期: 2026-07-20

Title:: Identification of FBL Gene Family, Mining of 100-seed Weight Related Genes and Haplotype Analysis in Soybean

作者:: 康朝锐¹; 韩佳音¹; 杨明亮¹; 吕子淼²; 章磊¹; 熊心¹; 蒋洪蔚²*; 陈庆山¹*; 1.东北农业大学农学院/教育部大豆生物学重点实验室，黑龙江哈尔滨 150030；2.吉林省农业科学院(中国农业科技东北创新中心)，吉林长春 130033

关键词:: 大豆; 百粒重; FBL; 表达模式; 单倍型分析; GmFBL24

DOI:: 10.11861/j.issn.1000-9841.2026.04.0035

摘要:: F-box蛋白是真核生物SCF E3泛素连接酶的重要组成部分，广泛参与植物的生长发育以及胁迫响应。为探究FBL基因家族在大豆百粒重调控过程中的作用，本研究采用生物信息学方法鉴定FBL家族基因，分析其共线性关系、理化性质及保守基序，利用RNA-seq数据分析大豆FBL基因的组织表达模式及籽粒发育前期的表达模式，选取籽粒发育前期特异表达的4个FBL基因进行qRT-PCR分析和单倍型分析，以阐释大豆FBL基因家族的结构、进化和功能。结果表明：共鉴定到44个大豆FBL基因，将其命名为GmFBL1~GmFBL44，编码的氨基酸数量为284~650 aa，等电点为5.16~8.99，其保守基序高度相似。物种内共线性分析结果表明该基因家族发生了53次复制事件，在进化过程中大豆FBL基因出现了分化。基因表达模式分析结果表明FBL基因家族中12个基因在大豆籽粒中特异表达，其中GmFBL14、GmFBL24、GmFBL25和GmFBL31在籽粒发育前期的两个亲本材料中的表达具有差异，其中GmFBL14和GmFBL25的表达量呈上升趋势，GmFBL24和GmFBL31的表达量先增加后降低。单倍型分析挖掘到GmFBL24在-386 bp处存在1处C/G突变，可能参与调控大豆百粒重。研究结果为解析研究大豆FBL基因家族在百粒重调控中的功能提供了理论依据。

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更新日期/Last Update: 1900-01-01