四川遂宁花叶、皱缩大豆病毒复合侵染分子鉴定及分析
- Title:
- Molecular Identification and Analysis of Complex Infection by Mosaic and Leaf Crinkle Soybean Viruses in Suining，Sichuan
- 摘要:
- 为了研究四川遂宁表现出花叶、皱缩等症状的大豆是否受病毒侵染及其病毒种类，采用小RNA深度测序技术检测四川遂宁侵染大豆的病毒种类，并利用RT-PCR和序列测序分析对结果进行验证。结果显示：大豆样品受到大豆花叶病毒（Soybean Mosaic Virus，SMV）和菜豆普通花叶病毒（Bean Common Mosaic Virus，BCMV）的复合侵染。通过RT-PCR克隆和测序后证实上述2种病毒的存在，并以四川遂宁分离物分别命名（SMV-SCSN和BCMV-SCSN）。进一步扩增、克隆测序和组装后获得了SMV-SCSN的全基因组。系统发育分析表明，BCMV-SCSN分离物聚类在地域和寄主多样性最丰富的第V类群，且与BCMV-PStV株系具有较近的亲缘关系；SMV-SCSN与来自于韩国大豆的Anseong分离物亲缘关系最近，聚为系统发育地位独特的Clade 8类群。本研究首次报道SMV和BCMV在四川遂宁地区对大豆的复合侵染危害，为深入解析两种病毒的复合侵染机制，培育兼抗性大豆种质并开展病害防控提供参考。
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