[1]邢兴华,王好让,李思梦,等.出苗期干旱胁迫下α-萘乙酸引发对大豆下胚轴线粒体电子传递与抗氧化代谢的影响[J].大豆科学,2026,(01):68-78.[doi:10.11861/j.issn.1000-9841.2026.01.0068]

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出苗期干旱胁迫下α-萘乙酸引发对大豆下胚轴线粒体电子传递与抗氧化代谢的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 期数: 2026年01期页码: 68-78 栏目: 出版日期: 2026-01-20

Title:: Influence of α-Naphthaleneacetic Acid Priming on Mitochondrial Electron Transport and Antioxidant Metabolism in Soybean Hypocotyls under Drought Stress at Emergence Stage

作者:: 邢兴华; 王好让; 李思梦; 徐泽俊; 齐玉军; 童飞; 晋彤彤; 王幸*; 江苏徐淮地区徐州农业科学研究所，江苏徐州 221131

关键词:: 大豆; 干旱胁迫; 下胚轴; α-萘乙酸; 电子传递; 抗氧化

DOI:: 10.11861/j.issn.1000-9841.2026.01.0068

摘要:: 为探讨α-萘乙酸（α-Naphthaleneacetic Acid,NAA）引发对出苗期干旱胁迫下大豆下胚轴伸长的调控机制，以大豆稳定品系徐9302为试验材料，经5 μmol·L^-1 NAA对种子进行引发处理后，采用盆栽称重控水法模拟干旱胁迫处理，测定大豆出苗率、下胚轴长度及呼吸速率，并分析了下胚轴线粒体中复合体活性、过氧化氢（H₂O₂）、抗氧化物质含量和抗氧化酶活性的变化。结果表明，NAA引发提高了干旱胁迫下大豆下胚轴长度和出苗率。在呼吸链复合体中，干旱胁迫对复合体Ⅰ活性抑制程度最大，同时提高了还原型烟酰胺腺嘌呤二核苷酸（Reduced Nicotinamide Adenine Dinucleotide, NADH）与氧化型烟酰胺腺嘌呤二核苷酸（Oxidized Nicotinamide Adenine Dinucleotide, NAD⁺）的比值（NADH/NAD⁺），致使下胚轴线粒体中H₂O₂和丙二醛含量分别增加49.2%~85.6%和25.7%~58.9%，造成线粒体氧化损伤。干旱胁迫下，NAA引发使得复合体Ⅰ活性和交替途径呼吸速率分别增加48.3%~108.1%和13.1%～38.3%，NADH/NAD⁺比值降低23.3%~32.6%，从源头上抑制了H₂O₂生成。此外，NAA引发还可提高抗坏血酸过氧化物酶、单脱氢抗坏血酸还原酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶活性，增加幅度分别为30.3%~51.1%、38.2%~57.5%、28.3%~29.3%和23.0%~75.6%，从而维持还原型抗坏血酸和谷胱甘肽的再生，促进线粒体中已产生H₂O₂的有效清除。综上，NAA引发通过协同调控线粒体电子传递与抗氧化系统，缓解了干旱胁迫诱导的线粒体氧化损伤，从而促进下胚轴伸长并提高出苗率。研究结果可为大豆出苗期干旱胁迫防控提供理论依据与技术参考。

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更新日期/Last Update: 1900-01-01