[1]曹天姿,王旭,王涛,等.邯豆系列大豆品种对不同SMV株系抗性的鉴定[J].大豆科学,2025,(06):55-61.[doi:10.11861/j.issn.1000-9841.2025.06.0055]

点击复制

邯豆系列大豆品种对不同SMV株系抗性的鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 期数: 2025年06期页码: 55-61 栏目: 出版日期: 2025-11-20

Title:: Identification of Resistance of Handou Series Soybean Varieties to Different SMV Strains

作者:: 曹天姿¹; 王旭²; 王涛²; 肖付明²; 孙天杰¹; 张洁¹; 王冬梅¹*; 1.河北农业大学生命科学学院，河北保定 071000；2.邯郸市农业科学院，河北邯郸 056000

关键词:: 大豆; SMV; 抗性分析; 表型观察; RT-qPCR

DOI:: 10.11861/j.issn.1000-9841.2025.06.0055

摘要:: 为深入挖掘河北省种植的大豆品种中抗大豆花叶病毒病的资源材料，以邯郸市农业科学院培育的28个邯豆系列品种为试验材料，接种河北省大豆主产区流行的SMV(Soybean Mosaic Virus)株系SC3、SC7、SC8和N3，通过观察发病表型和鉴定病情等级，筛选出优质的抗SMV大豆种质资源。结果显示：邯豆系列大豆品种整体对SC8、N3和SC3株系的抗性弱于对SC7株系的抗性，其中邯豆15对各个SMV株系的抗性均较强，邯豆17对各个 SMV株系的抗性均较弱；RT-qPCR分析不同抗性品种中抗SMV相关基因（GmPR1、GmWRKY164和GmRD22）在转录水平的表达量表明，3个基因在抗性较强的邯豆15中的表达量均明显高于在抗性较差的邯豆17中的表达量，与表型鉴定结果一致。结果表明邯豆系列大豆品种对SMV的抗性差异可能与抗病相关基因的表达水平密切相关，可为广泛筛选大豆抗花叶病毒资源提供参考依据。

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]郭丹丹,陈海峰,杨中路,等.大豆对SMV SC-3株系的抗性遗传和QTL分析[J].大豆科学,2012,31(04):511.[doi:10.3969/j.issn.1000-9841.2012.04.001]
　GUO Dan-dan,CHEN Hai-feng,YANG Zhong-lu,et al.Analysis of Resistance Inheritance to Soybean Mosaic Virus（SMV）Strain SC-3 and Mapping Related QTL in Soybean[J].Soybean Science,2012,31(06):511.[doi:10.3969/j.issn.1000-9841.2012.04.001]
[12]栾晓燕,陈怡,杜维广,等.不同抗性大豆品种(系)感染SMV后可溶性糖和游离氨基酸的研究[J].大豆科学,2000,19(04):356.[doi:10.11861/j.issn.1000-9841.2000.04.0356]
　[J].Soybean Science,2000,19(06):356.[doi:10.11861/j.issn.1000-9841.2000.04.0356]
[13]方飞,杨云华,王丽群,等.大豆类受体激酶基因GmNIK的克隆与表达分析[J].大豆科学,2019,38(05):704.[doi:10.11861/j.issn.1000-9841.2019.05.0704]
　FANG Fei,YANG Yun-hua,WANG Li-qun,et al.Cloning and Analysis of Receptor-like Kinase Gene GmNIK in Soybean[J].Soybean Science,2019,38(06):704.[doi:10.11861/j.issn.1000-9841.2019.05.0704]
[14]陈诚,伏荣桃,王剑,等.四川遂宁花叶、皱缩大豆病毒复合侵染分子鉴定及分析[J].大豆科学,2026,(01):124.[doi:10.11861/j.issn.1000-9841.2026.01.0124]

更新日期/Last Update: 1900-01-01