[1]姜姊琪,鲍重杰,商雨欣,等.大豆品种黑农531抗灰斑病的生理、遗传及转录特征分析[J].大豆科学,2025,(05):136-145.[doi:10.11861/j.issn.1000-9841.2025.05.0136]
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大豆品种黑农531抗灰斑病的生理、遗传及转录特征分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 期数: 2025年05期 页码: 136-145 栏目: 出版日期: 2025-09-20
Title:
Comprehensive Analysis of Physiological, Genetic and Transcriptional Characteristics of Soybean Variety Heinong 531 in Resistance to Frogeye Leaf Spot
作者:
姜姊琪1; 2; 鲍重杰3; 商雨欣3; 周润南1; 尤佳1; 任洪雷1; 王家军1*
1.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨 150086；2.黑龙江八一农垦大学 农学院，黑龙江 大庆 163319；3.黑龙江大学 现代农业与生态环境学院，黑龙江 哈尔滨 150080
关键词:
大豆; 灰斑病; 黑农531; 分子标记; 生理指标; 抗病基因
DOI:
10.11861/j.issn.1000-9841.2025.05.0136
摘要:
大豆灰斑病（Cercospora sojina Hara）是一种全球性病害，也是我国大豆生产中的主要病害，严重影响大豆的产量与品质。黑农531田间表现为高抗大豆灰斑病，为明确黑农531对灰斑病的抗性特征，本研究选取23份具有不同抗性水平的大豆种质资源，包括高抗、中抗、感病和高感材料作为对照，人工接种灰斑病菌（Cercospora sojina）并进行系统分析。同时利用SSR分子标记对已知抗病基因位点进行基因型分析，探究基因型与抗病表型的相关性。在生理生化层面，进行生理指标测定，分析黑农531的抗灰斑病生理机制。此外，采用qRT-PCR技术检测抗病相关基因的表达模式。结果表明：黑农531的病情指数为2.2，表现为高抗水平；分子标记检测显示该品种在Satt565位点具有特异性抗病条带；与高感材料相比，黑农531中超氧化物歧化酶、过氧化氢酶和多酚氧化酶活性均显著升高；接种病原菌后，抗病基因GmRLK、GmTGA06和GmSOD1的表达量均有明显上调。本研究为黑农531在大豆抗病育种中的应用提供了重要依据，并为大豆抗灰斑病品种的选育奠定了理论与种质基础。

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更新日期/Last Update: 1900-01-01