[1]王雅楠,王大刚,李杰坤,等.基于代谢组和转录组的大豆CMS花粉败育机制研究[J].大豆科学,2025,(05):110-118.[doi:10.11861/j.issn.1000-9841.2025.05.0110]
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基于代谢组和转录组的大豆CMS花粉败育机制研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 期数: 2025年05期 页码: 110-118 栏目: 出版日期: 2025-09-20
Title:
Research on the Mechanism of Soybean CMS Pollen Abortion Based on Metabolomics and Transcriptomics
作者:
王雅楠; 王大刚; 李杰坤; 吴倩; 胡国玉; 黄志平*
安徽省农业科学院 作物研究所/安徽省农作物品质改良重点实验室，安徽 合肥 230031
关键词:
大豆; 细胞质雄性不育; 代谢组; 转录组; 黄酮类化合物
DOI:
10.11861/j.issn.1000-9841.2025.05.0110
摘要:
杂种优势利用是提高大豆产量的有效策略之一，细胞质雄性不育（Cytoplasmic-nuclear Male Sterility，CMS）在大豆杂种优势利用中具有重要作用，为了阐明大豆CMS发生的分子机制，利用大豆CMS系W931A及其保持系W931B的单核小孢子（Uninucleate Microspore，UM）和二胞花粉期（Binucleate Pollen，BP）花蕾进行代谢组学研究，并与转录组数据联合分析，挖掘大豆CMS相关基因及代谢途径。代谢组分析结果显示，在CMS系W931A的UM和BP阶段分别鉴定到147和305个差异代谢物，主要包括脂质及类脂分子化合物、苯丙烷及聚酮化合物和有机杂环化合物等。转录组和代谢组联合分析揭示，差异表达基因（Differentially Expressed Genes，DEGs）和差异代谢物主要参与黄酮类化合物生物合成、苯丙氨酸代谢和苯丙烷类生物合成。在花粉发育过程中，黄酮类及衍生物的合成被显著抑制，F3H（Glyma.01G166200）和FLS（Glyma.06G110600）基因在CMS系W931A中显著下调表达，推测其在相应代谢途径中发挥关键调控作用，导致花药中相关代谢物的差异，进而引发不育系花粉的败育。研究结果有助于构建大豆CMS分子调控网络，并推动大豆CMS分子机制的研究进程。

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更新日期/Last Update: 1900-01-01