大豆盐胁迫和干旱胁迫响应EXP基因鉴定及GmEXLB2克隆和诱导表达分析
- Title:
- Identification of Salt and Drought Stress-Responsive EXP Genes and Cloning and Induced Expression Analysis of GmEXLB2 in Soybean
- 摘要:
- 大豆是一种重要的粮油兼用经济作物，其产量常受到干旱和高盐等非生物胁迫的严重影响。扩展蛋白（expansin，EXP）是一类在植物中发挥重要功能的蛋白质，能够引起植物细胞壁松弛。这类蛋白通过对植物细胞壁的调控，在非生物胁迫中发挥重要作用。为挖掘大豆中同时响应盐胁迫和干旱胁迫的EXPs，本研究基于前期盐胁迫和干旱胁迫处理的转录组数据，利用韦恩图、生物信息学分析、实时荧光定量PCR和基因克隆方法对EXPs基因进行了系统分析。结果表明：共有49个GmEXPs响应盐胁迫，21个GmEXPs响应干旱胁迫，其中15个GmEXPs基因受到盐胁迫和干旱胁迫的共同调控。进一步分析发现，这15个GmEXPs基因的启动子区域含有多个与植物逆境胁迫和植物激素应答相关的顺式作用元件。本研究在大豆品种齐黄34根系中克隆到受干旱胁迫和盐胁迫显著诱导的基因GmEXLB2，该基因编码expansin-like B蛋白。GmEXLB2蛋白序列与野生大豆扩展蛋白具有较高的氨基酸序列同源性，该蛋白含有保守的Expansin结构域，其二级结构主要由α-螺旋和无规则卷曲组成。GmEXLB2基因在下胚轴中表达水平最高，且盐胁迫和干旱胁迫处理显著诱导根中GmEXLB2的表达。此外，GmEXLB2基因的表达受植物激素调控，其中ACC、GA3和ABA处理均能诱导GmEXLB2基因表达。结果说明GmEXLB2基因在响应盐胁迫和干旱胁迫中发挥重要作用，该基因可能通过植物激素信号参与大豆盐胁迫和干旱胁迫响应。结果可为探究大豆EXLB基因响应逆境机理提供新的思路，也为深入研究GmEXLB2基因的抗旱和耐盐功能奠定了理论基础。
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]陈 昀,郑焕明,张 竞,等.以hemin为基础的物质联用缓解盐胁迫对大豆种子萌发的抑制[J].大豆科学,2013,32(05):640.[doi:10.11861/j.issn.1000-9841.2013.05.0640]
CHEN Yun,ZHENG Huan-ming,ZHANG Jing,et al.Hemin-based Combinations of Exogenous Substances Alleviate the Inhibition of Soybean Seed Germination under Salt Stress[J].Soybean Science,2013,32(05):640.[doi:10.11861/j.issn.1000-9841.2013.05.0640]
[12]马淑英 尹田夫 袁鹰 刘德璞 刘艳芝.盐胁迫对大豆发育子叶愈伤组织的生化影响[J].大豆科学,1997,16(03):227.[doi:10.11861/j.issn.1000-9841.1997.03.0227]
Ma ShuyingYin Tanfu Yua n YingLiu DepuLiu Ya nzhing.EFFECT OF SALT STRESS ON SOYBEAN COTYLEDON CALLUSTISSUE DEVELOPMENT[J].Soybean Science,1997,16(05):227.[doi:10.11861/j.issn.1000-9841.1997.03.0227]
[13]常汝镇,陈一舞,邵桂花,等.盐对大豆农艺性状及籽粒品质的影响[J].大豆科学,1994,13(02):101.[doi:10.11861/j.issn.1000-9841.1994.02.0101]
[J].Soybean Science,1994,13(05):101.[doi:10.11861/j.issn.1000-9841.1994.02.0101]
[14]徐芬芬,楚婕妤,刘誉,等.盐胁迫对大豆种子萌发过程中吸水和水解酶活性的影响[J].大豆科学,2017,36(01):74.[doi:10.11861/j.issn.1000-9841.2017.01.0074]
XU Fen-fen,CHU Jie-yu,LIU Yu,et al.Effects of Salt Stress on Water Uptake and Hydrolytic Enzyme Activities During Soybean Seed Germination[J].Soybean Science,2017,36(05):74.[doi:10.11861/j.issn.1000-9841.2017.01.0074]
[15]侯鹏浩,杨万明,杜维俊,等.不同程度盐胁迫对大豆苗期生物量及生理指标的影响[J].大豆科学,2020,39(03):422.[doi:10.11861/j.issn.1000-9841.2020.03.0422]
HOU Peng-hao,YANG Wan-ming,DU Wei-jun,et al.Effects of Different Degree Salt Stress on Biomass and Physiological Indexes of Soybean Seedling[J].Soybean Science,2020,39(05):422.[doi:10.11861/j.issn.1000-9841.2020.03.0422]
[16]王玉斌,刘薇,张彦威,等.乙烯对大豆幼苗盐胁迫响应的调控机制研究[J].大豆科学,2022,41(05):580.[doi:10.11861/j.issn.1000-9841.2022.05.0580]
WANG Yu-bin,LIU Wei,ZHANG Yan-wei,et al.Regulation Mechanism in Soybean Seedling Response to Salt Stress of Ethylene[J].Soybean Science,2022,41(05):580.[doi:10.11861/j.issn.1000-9841.2022.05.0580]
[17]王玉斌,张志贤,张辰淼,等.大豆盐胁迫响应NRT基因的鉴定及GmNRT1.5A的克隆和表达分析[J].大豆科学,2023,42(06):674.[doi:10.11861/j.issn.1000-9841.2023.06.0674]
[18]邹平,杨霞,于喆妍,等.不同种类海藻多糖对盐胁迫下大豆幼苗的促进作用[J].大豆科学,2024,43(01):73.[doi:10.11861/j.issn.10009841.2024.01.0073]
[19]何芳蕾,李兰馨,卢秋连,等.大豆磷脂酶D家族基因全基因组鉴定及耐盐性分析[J].大豆科学,2024,43(06):695.[doi:10.11861/j.issn.1000-9841.2024.06.0695]
[20]张兴政,刘鑫,黄浩捷,等.大豆ACO家族基因鉴定、生物信息学分析及盐胁迫下表达模式分析[J].大豆科学,2025,(03):27.[doi:10.11861/j.issn.1000-9841.2025.03.0027]